Time-resolved fluorescence detection of oligonucleotide hybridization on a single microparticle: Covalent immobilization of oligonucleotides and quantitation of a model system

Bioconjugate Chemistry

Volumn 8, Issue 2, 1997, Pages 232-237

  Hakala, Harri ^a   Lönnberg, Harri ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLIC MONOMERS; CHLORINE COMPOUNDS; EUROPIUM COMPOUNDS; FLUORESCENCE; PROBES;

COVALENT IMMOBILIZATION; FLUORESCENCE DETECTION; GLYCIDYL METHACRYLATE-ETHYLENE DIMETHACRYLATE; HYBRIDISATION; MICRO PARTICLES; MODELLING SYSTEMS; OLIGONUCLEOTIDE HYBRIDIZATION; OLIGONUCLEOTIDE PROBES; TIME-RESOLVED FLUORESCENCE;

OLIGONUCLEOTIDES;

EID: 0342618455     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc9700143     Document Type: Article

References (15)

1. Saiki, R., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491.
2. Southern, E. M., Maskos, U., and Elder, J. K. (1992) Analyzing and comparing nucleic acid sequences by hybridization to arrays of oligonucleotides: Evaluation using experimental models. Genomics 13, 1008-1017.
3. Southern, E. M., Case-Green, S. C., Elder, J. K., Johnson, M., Mir, K. U., Wang, L., and Williams, J. C. (1994) Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids. Nucleic Acids Res. 22, 1368-1373.
4. Guo, Z., Guilfoyle, R. A., Thiel, A. J., Wang, R., and Smith, L. M. (1994) Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acids Res. 22, 5456-5465.
5. Soini, E. (1991) Biospecific multianalyte assay method. U.S. Pat. 5,028,545.
6. Lövgren, T., Heinonen, P., Lehtinen, P., Hakala, H., Heinola, J., Harju, R., Takalo, H., Mukkala, V.-M., Schmid, R., Lönnberg, H., Pettersson, K., and Iitiä, A. (1996) Sensitive bioaffinity assays on individual microparticles using time-resolved fluorometry. Clin. Chem. (submitted for publication).
7. Ugelstad, J., Berge, A., Ellingsen, T., Schmid, R., Nilsel, T.-N., Mørk, P. C., Stenstad, P., Hornes, E., and Olsvik, Ø. (1992) Preparation and application of new monosized polymer particles. Prog. Polym. Sci. 17, 87-161.
8. Mukkala, V.-M., Helenius, M., Hemmilä, I., Kankare, J., and Takalo, H. (1993) Development of luminescent europium(III) and terbium(III) chelates of 2,2′:6′,2″-terpyridine derivatives for protein labelling. Helv. Chim. Acta 76, 1361-1378.
9. Hovinen, J., Guzaev, A., Azhayeva, E., Azhayev, A., and Lönnberg, H. (1995) Imidazole tethered oligodeoxyribonucleotides: synthesis and RNA cleaving activity. J. Org. Chem. 60, 2205-2209.
10. Brocklehurst, K., and Little, G. (1973) Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. Biochem. J. 133, 67-80.
11. Salo, H., Guzaev, A., Azhayev, A., and Lönnberg, H. (1996) Disulfide tethered solid supports for oligonucleotide synthesis and a comparative study on their resistance to ammonolysis. Collect Czech. Chem. Commun. 61 (special issue), S110-111.
12. Hovinen, J., Guzaev, A., Azhayev, A., and Lönnberg, H. (1994) Novel solid supports for the preparation of 3′-derivatized oligonucleotides: introduction of 3′-alkylphosphate tether groups bearing amino, carboxy, carboxamido, and mercapto fuctionalities. Tetrahedron 50, 7203-7218.
13. 3+)-labeled oligo DNA hybridization probes. Nucleosides Nucleotides 7, 655-659.
14. Lövgren, T., Iitiä, A., Hurskainen, P., and Dahlen, P. (1995) Detection of lanthanide chelates by time-resolved fluorescence. In Blotting and Sentencing (L. Kricka, Ed.) pp 350-358, Academic Press, San Diego, CA.
15. Hemmilä, I., Dakubu, S., Mukkala, V.-M., Siitari, H., and Lövgren, T. (1984) Europium as a label in time-resolved immunofluorometric assays. Anal. Biochem. 137, 335-343.