Oxidative and random cleavage of DNA by the novel iron(II) complex capable of yielding an iron(III) hydroperoxide intermediate

Chemistry Letters

Volumn , Issue 1, 1998, Pages 39-40

  Ishikawa, Yoshinobu ^b   Morishita, Yuko ^b   Yamamoto, Teruo ^b   Kurosaki, Hiromasa ^b   Goto, Masafumi ^b   Matsuo, Hiroaki ^a   Sugiyama, Masanori ^a  

^a HIROSHIMA UNIV SCHOOL OF MEDICINE   (Japan)

^b KUMAMOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0032363269     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.1998.39     Document Type: Article

References (20)

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17. 2 in 20 mM Tris-HCl buffer (pH 7.5) for 10 min at 37 °C. After loading buffer [95% formamide, 20 mM EDTA, 0.05% bromophenol blue and 0.05% xylene cyanol] was added to the reaction mixture, the samples were heated at 95°C for 3 min, then cooled on an ice-bath, loaded on a 6% Long Ranger polyacrylamide (FMC Bioproducts) denaturing (7 M urea) gel and electrophoresed at 35 W for 4 h. The DNA was electrotransfered onto Biodyne B nylon membrane (Pall BioSupport Corp.) and 5'-biotinylated DNA bands were detected by Phototope-Star Detection Kit (New England Biolabs, Inc.). Sequence ladder was generated with primer 1 using pUC19 as a template in dideoxy sequencing reactions.
18. 2 (30% aqueous, 1.0 mmol), cyclohexane (10.0 mmol) or cyclohexene (10.0 mmol).
19. Spectrometer settings: microwave frequency, 8.99 GHz; microwave power, 1.83 mW; modulation frequency, 100 kHz; modulation width, 0.63 mT.
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