Polyelectrolyte complexes as vehicles for affinity precipitation of proteins

Journal of Biotechnology

Volumn 52, Issue 1, 1996, Pages 1-10

  Dissing, Ulla ^a   Mattiasson, Bo ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

affinity precipitation; polyacrylic acid sodium salt; polyelectrolyte complex; polyethylene imine

Indexed keywords

BIOTECHNOLOGY; CARRIER CONCENTRATION; DESORPTION; ENZYMES; NUCLEIC ACIDS; ORGANIC POLYMERS; PH EFFECTS; PRECIPITATION (CHEMICAL); PROTEINS; SEPARATION; SOLUBILITY; STOICHIOMETRY;

AFFINITY PRECIPITATION; LACTATE DEHYDROGENASE; POLYACRYLIC ACID SODIUM SALT; POLYETHYLENE IMINE;

POLYELECTROLYTES;

LACTATE DEHYDROGENASE;

AFFINITY CHROMATOGRAPHY; ANIMAL TISSUE; ARTICLE; CATTLE; COMPLEX FORMATION; GEL ELECTROPHORESIS; HEART; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN PURIFICATION; TECHNIQUE;

ACRYLIC RESINS; ANIMALS; CATTLE; ELECTROLYTES; L-LACTATE DEHYDROGENASE; NUCLEIC ACIDS; POLYETHYLENES; PRECIPITATION; PROTEINS; TRIAZINES;

ANIMALIA; BOS TAURUS;

EID: 0030606170     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(96)01594-5     Document Type: Article

References (19)

1. Atkinson, A. and Jack, G.W. (1973) Precipitation of nucleic acids with polyethyleneimine and the chromatography of nucleic acids and proteins on immobilized polyethyleneimine. Biochim. Biophys. Acta 308, 41-52.
2. Bradford, M. (1976) Anal. Biochem. 72, 248-254.
3. Cordes, R.M., Sims, W.B. and Glatz, C.E. (1990) Biotechnol. Prog. 6, 283-285.
4. Dean, P.D.G. and Watson, D.H. (1979) Protein purification using immobilized triazine dyes. J Chromatog. 165, 301-319.
5. Decker, Lillian A. (1977) Worthington Enzyme Manual, Freehold, New Jersey, USA.
6. Fujii, M. and Taniguchi, M. (1991) Application of reversibly soluble polymers in bioprocessing. Trends Biotechnol. 9, 191-196.
7. Galaev, I.Yu. and Mattiasson, B. (1993) Affinity thermoprecipitation: contribution of the efficiency of ligand-protein interaction and access of the ligand. Biotechnol. Bioeng. 41, 1101-1106.
8. Guoqiang, D., Batra, R., Kaul, R., Gupta, M.N. and Mattiasson, B. (1995) Alternative modes of precipitation of Eudragit S-100: a potential ligand carrier for affinity precipitation of protein. Bioseparation 5, 339-350.
9. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
10. 13C study of the structure of polyethyleneimine. Eur. Polymer J. 9, 559-565.
11. Milburn, P., Bonnerjea, M., Hoare, M. and Dunnill, P. (1990) Selective flocculation of nucleic acids, lipids and colloidal particles from a yeast cell homogenate by polyethyleneimine and its scale-up. Enzyme Microb. Technol. 12, 527-532.
12. Philipp, B., Dautzenberg, H., Linow, K.-J., Kötz, J. and Dawydoff, W. (1989) Polyelectrolyte complexes - recent developments and open problems. Prog. Polymer Sci. 14, 91-172.
13. Quadri, F. (1985) The reactive triazine dyes: their usefulness and limitations in protein purifications. Trends Biotechnol. 3, 7-12.
14. Schneider, M., Guillot, C. and Lamy, B. (1981) The affinity precipitation technique: Application to the isolation and purification of trypsin from bovine pancreas. Ann. N.Y. Acad. Sci., 369, 257-263.
15. Skabrahová, Z., Kovár, J., Glatz, Z., Turánek, J. and Kahle, V. (1987) High-performance liquid chromatographic purification of the H4 and MH3 isoenzymes of lactate dehydrogenases from beef heart. J. Chromatogr. 408, 372-377.
16. Smalley, J.W., Birss, A.J., McKee A.S. and Marsh, P.D. (1993) J. Gen. Microbiol. 139, 2145-2150.
17. Stead, C.V. (1991) The use of dyes in protein purification. Bioseparation 2, 129-136.
18. Taniguchi, M., Kobayashi, M., Natsui, K. and Fuiji, M. (1989) Purification of staphylococcal protein A by affinity precipitation using a reversibly soluble-insoluble polymer with human IgG as a ligand. J. Ferment. Bioeng. 68, 32-36.
19. Thompson, S.T. and Stellwagen, E. (1976) Binding of Cibacron blue F3GA to proteins containing the dinucleotide fold. Proc. Natl. Acad. Sci. USA 73, 361-365.