Imaging of rotational Brownian motion of biomolecules in living cells using fluorescence depolarization microscopy

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 3260, Issue , 1998, Pages 84-95

  Toyonaga, Shuji ^a   Nakano, Yoshitaro ^a  

^a Laboratory of Molecular Biophotonics   (Japan)

Author keywords

Biomolecules; Fluorescence depolarization technique; Living cell; Rotational Brownian motion; Time resolved measurement; Two dimensional imaging; Two photon excitation measurement

Indexed keywords

ANISOTROPY; BROWNIAN MOVEMENT; CELLS; CHARGE COUPLED DEVICES; FLUORESCENCE; MICROSCOPES; MOLECULES; PHOTONS;

FLUORESCENCE DEPOLARIZATION TECHNIQUE (FDT);

MEDICAL IMAGING;

EID: 0032225603     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.307085     Document Type: Conference Paper

References (25)

1. Light on high-throughput screening: Fluorescence-based assay technologies
2. Fluorescence spectroscopy as a tool to investigate protein interactions
3. Fluorescence spectrometry in studies of carbohydrate-protein interactions
4. Polarization de la lumiere de fluorescence. Vie moyenne de molecules dans l'etat excite
5. Rotational Brownian motion and polarization of the fluorescence of solutions
6. Fluorescence polarization- A new tool for cell and molecular biology
7. Fluorescence polarization assays for the detection of proteases and their inhibitors
8. Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization
9. Mapping of fluorescence anisotropy in living cells by ratio imaging: Application to cytoplasmic viscosity
10. 2+-induced exocytosis: Microscopic analysis with fluorescence anisotropy
11. Fluorescence anisotropy imaging microscopy maps calmodulin binding during cellular contraction and locomotion
12. The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy
13. Time-resolved fluorescence intensity and anisotropy decay of 2,5-diphenyloxazole by two-photon excitation and frequency-domain fluorometry
14. Anomalous differential polarized phase angles for two-photon excitation with isotropic depolarizing rotations
15. Tryptophan fluorescence intensity and anisotropy decays of human serum albumin resulting from one-photon and two-photon excitation
16. Two photon-induced fluorescence intensity and anisotropy decays of diphenylhexatriene in solvents and lipid bilayers
17. Fluorescence intensity and anisotropy decay of the 4′,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation
18. Characterization of p-bis (O-methylstyryl) benzene as a lifetime and anisotropy decay standard for two-photon induced fluorescence
19. Fluorescence intensity and anisotropy decays of the DNA stain Hoechst 33342 resulting from one-photon and two-photon excitation
20. Fluorescence anisotropy of tyrosine using one- and two-photon excitation
21. Two-photon excitation by the evanescent wave from total internal reflection
22. Comparison of two-photon excitation laser scanning microscopy with UV-confocal laser scanning microscopy in three dimensional calcium imaging using the fluorescence indicator Indo-1
23. Modulation of synapse formation by cyclic adenosine monophosphate
24. Membrane asymmetry in epithelia: Is the tight junction a barrier to diffusion in the plasma membrane?