Quantitative immunogold cytochemistry reveals sources of glutamate release in inner ear ischemia

Acta Oto-Laryngologica, Supplement

Volumn , Issue 539, 1998, Pages 48-51

  Matsubara, Atsushi ^a   Kawabata, Yuko ^a   Takumi, Yutaka ^a   Usami, Shin Ichi ^a   Shinkawa, Hideichi ^a   Haruta, Atsushi ^b   Matsuda, Keiji ^b   Tono, Tetsuya ^b  

^a HIROSAKI UNIVERSITY   (Japan)

^b UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

Glutamate; Guinea pig; Immunocytochemistry; Immunogold; Ischemia; Organ of Corti; Post embedding

Indexed keywords

GLUTAMIC ACID;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; CONFERENCE PAPER; CONTROLLED STUDY; CORTI ORGAN; DENDRITE; HAIR CELL; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD STAINING; INNER EAR DISEASE; ISCHEMIA; NEUROTRANSMITTER RELEASE; NONHUMAN; PERILYMPH; PRIORITY JOURNAL; ANIMAL; ARTICLE; CYTOLOGY; GUINEA PIG; IMMUNOHISTOCHEMISTRY; METABOLISM; PATHOPHYSIOLOGY; VASCULARIZATION;

ANIMALS; GLUTAMIC ACID; GUINEA PIGS; HAIR CELLS, INNER; IMMUNOHISTOCHEMISTRY; ISCHEMIA;

EID: 0032216820     PISSN: 03655237     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (24)

1. Eybalin M. Neurotransmitters and neuromodulators of the mammalian cochlea. Physiol Rev 1993; 73: 309-73.
2. Ottersen OP, Takumi Y, Matsubara A, Landsend AS, Lakke JH, Usami S. Molecular organization of a type of periphetralglutamate synapse: the afferent synapses of hair cells in the inner ear. Prog Neurobiol 1998; 54: 127-48.
3. Altschuler RA, Sheridan CE, Horn JW, Wenthold RJ. Immunocytochemical localization of glutamate immunoreactivity in the guinea pig cochlea. Hear Res 1989; 42: 167-74.
4. Usami S, Osen KK, Zhang N, Ottersen OP. Distribution of glutamate-like and glutamine-like immunoreactivities in the rat organ of Corti: a light microscopic and semiquantitative electron microscopic analysis with a note on the localization of aspartate. Exp Brain Res 1992; 91: 1-11.
5. Kataoka Y, Ohmori H. Activation of glutamate receptors in response to membrane depolarization of hair cells isolated from chick cochlea. J Physiol (Lond) 1994; 477: 403-14.
6. Rothman SM, Olney JW. Glutamate and the pathophysiology of hypoxic-ischemic brain damage. Ann Neurol 1986; 19: 105-11.
7. Ottersen OP, Laake JH, Reichelt W, Haug FM, Torp R. Ischemic disruption of glutamate homeostasis in the brain: quantitative immunocytochemical analyses. J Chem Neuroanat 1996; 12: 1-14.
8. Pujol R, Rebillard G, Puel J-L, Lenoir M, Eybalin M, Recasens M. Glutamate neurotoxicity in the cochlea: a possible consequence of ischemic or anoxic conditions occurring in ageing. Acta Otolarungol (Stockh) 1991; Suppl 476: 32-6.
9. Pujol R, Puel J-L, D'aldin CG, Eybalin M. Pathophysiology of the glutamatergic synapses in the cochlea. Acta Otolarungol (Stockh) 1993; 113: 330-4.
10. Puel JL, Pujol R, Tribillac F, Ladrech S, Eybalin M. Excitatory amino acid antagonists protect cochlear auditory neurons from excitotoxicity. J Comp Neurol 1994; 341: 241-56.
11. Billett TE, Thorne PR, Gavin JB. The nature and progression of injury in the organ of Corti during ischemia. Hear Res 1989; 41: 189-98.
12. Hakuba N, Gyo K, Yanagihara N, Mitani A, Kataoka K. Efflux of glutamate into the perilymph of the cochlea following transient ischemia in the gerbil. Neurosci Lett 1997; 43: 69-71.
13. Anniko M, Lundquist P-G. Temporal bone morphology after systemic arterial perfusion or intralabyrinthine in situ immersion: hair cells of the vestibular organs and the cochlea. Micron 1980; 11: 73-83.
14. Wersäll J. Studies on the structure and innervation of the sensory epithelium of the cristae ampullaris in the guinea pig. Acta Otolaryngol Suppl (Stockh) 1956; 126: 185.
15. Matsubara A, Laake JH, Davanger S, Usami S, Ottersen OP. Organization of AMPA receptor subunits at a glutamate synapse: a quantitative immunogold analysis of hair cell synapses in the inner ear. J Neurosci 1996; 16: 4457-67.
16. Ji Z, Aas J-E, Lakke J, Walberg F, Ottersen OP. An electron microscopic immunogold analysis of glutamate and glutumine in terminals of rat spinocerebellar fibers. J Comp Neurol 1991; 307: 296-310.
17. Ottersen OP. Post-embedding light- and electron-microscopic immunocytochemistry of amino acids: description of a new model system allowing identical conditions for specificity testing and tissue processing. Exp Brain Res 1987; 69: 167-74.
18. Blackstad TW, Nanci A, Kan FWK. MORFOREL, a computer program for two-dimensional analysis of micrographs of biological specimens, with emphasis on immunogold preparations. Comput Biol Med 1990; 20: 15-34.
19. 2+-independent release of glutamate during anoxia in isolated nerve terminals (synaptosomes). J Neurochem 1988; 50: 1322-4.
20. 2+-concentration in isolated nerve terminals following metabolic inhibition: possible relevance to hypoglycaemia and anoxia. Neuroscience 1988; 27: 175-82.
21. Nicholls D, Attwell D. The release and uptake of excitatory amino acids. TiPS 1990; 11: 462-8.
22. Barbour B, Brew H, Attwell D. Electrogenic glutamate uptake in glial cells is activated by intracellular potassium. Nature 1988; 335: 433-5.
23. Szatkowski M, Barbour B, Attwell D. Non-vesicular release of glutamate from glial cells by reversed electrogenie glutamate uptake. Nature 1990; 348: 443-5.
24. Furness D, Lehre KP. Immunocytochemical localization of a high-affinity glutamate-aspartate transporter, GLAST, in the rat and guinea pig cochlea. Eur J Neurosci 1997; 9: 1961-9.