Immunocytochemical localization of a high-affinity glutamate-aspartate transporter, GLAST, in the rat and guinea-pig cochlea

European Journal of Neuroscience

Volumn 9, Issue 9, 1997, Pages 1961-1969

  Furness, David N ^a   Lehre, Knut P ^b  

^a KEELE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

Hair cells; Neurotransmitters; Organ of Corti; Spiral ganglion

Indexed keywords

ASPARTIC ACID; BRAIN PROTEIN; CARRIER PROTEIN; GLUTAMATE ASPARTATE TRANSPORTER; GLUTAMIC ACID; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CELL MEMBRANE; COCHLEA; COCHLEAR NERVE; CONTROLLED STUDY; CORTI ORGAN; ELECTRON MICROSCOPY; GUINEA PIG; HAIR CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; MICROSCOPY; MOLECULAR CLONING; MYELIN SHEATH; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; SATELLITE CELL; SENSORY NERVE CELL; SPIRAL GANGLION; SYNAPSE;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT SYSTEM X-AG; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL TRANSPORT; GUINEA PIGS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; ORGAN OF CORTI; RATS; RATS, WISTAR; SPIRAL GANGLION; SUBCELLULAR FRACTIONS;

EID: 0030929239     PISSN: 0953816X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1460-9568.1997.tb00763.x     Document Type: Article

References (51)

1. Altschuler, R. A., Sheridan, C. E., Horn, J. W. and Wenthold, R. J. (1989) Immunocytochemical localization of glutamate immunoreactivity in the guinea pig cochlea. Hearing Res., 42, 167-174.
2. Billet, T. E., Thorne, P. R. and Gavin, J. B. (1989) The nature and progression of injury in the organ of Corti during ischemia. Hearing Res., 41, 189-198.
3. Bobbin, R. P. (1979) Glutamate and aspartate mimic the afferent transmitter in the cochlea. Exp. Brain Res., 34, 389-393.
4. Chaudhry, F. A., Lehre, K. P., Van Lookeren-Campagne, M., Ottersen, O. P., Danbolt, N. C. and Storm-Mathisen, J. (1995) Glutamate transporters in glial plasma membranes: highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry. Neuron, 15, 711-720.
5. Chiu, S. Y. and Kriegler, S. (1994) Neurotransmitter-mediated signalling between axons and glial cells. Glia, 11, 191-200.
6. Comis, S. D. and Leng, G. (1979) Action of putative neurotransmitters in the guinea-pig cochlea. Exp. Brain Res., 36, 119-128.
7. Danbolt, N. C. (1994) The high affinity uptake system for excitatory amino acids in the brain. Prog. Neurobiol., 44, 377-396.
8. Devau, G., Lehouelleur, J. and Sans, A. (1993) Glutamate receptors on type I vestibular hair cells of guinea pig. Eur. J. Neurosci., 5, 1210-1217.
9. Drescher, M. J., Drescher, D. G. and Medina, J. E. (1983) Effects of sound stimulation at several levels on concentrations of primary amines, including neurotransmitter candidates, in perilymph of the guinea pig inner ear. J. Neurochem., 41, 309-320.
10. Ehrenberger, K. and Felix, D. (1991) Glutamate receptors in afferent cochlear neurotransmission in guinea pigs. Hearing Res., 52, 73-80.
11. Eybalin, M. (1993) Neurotransmitters and neuromodulators of the mammalian cochlea. Physiol. Rev., 73, 309-373.
12. 3H]L-glutamine uptake in the guinea-pig cochlea. Neuroscience, 9, 863-871.
13. Eybalin, M., Norenberg, M. D. and Renard, N. (1996) Glutamine synthetase and glutamate metabolism in the guinea pig cochlea. Hearing Res., 101, 93-101.
14. Felix, D. and Ehrenberger, K. (1990) A microiontophoretic study of the role of excitatory amino acids at the afferent synapse of mammalian hair cells. Eur. Arch. Oto-Rhino-Laryngol., 248, 1-3.
15. Furness, D. N., Danbolt, N. C., Lehre, K. P., Osen, K. K., Storm-Mathisen, J., Ottersen, O. P. and Hackney, C. M. (1993) Distribution of glutamine, glutamate and glutamate-transporters in the mammalian cochlea. Br. J. Audiol., 27, 334-335.
16. Gallo, V. and Russell, J. T. (1995) Excitatory amino-acid receptors in glia - different subtypes for distinct functions. J. Neurosci. Res., 42, 1-8.
17. Gundersen, H. J. G., Bendtsen, T. F., Korbo, L., Marcussen, N., Møller, A., Nielsen, K., Nyengaard, J. R., Pakkenberg, B., Sørensen, F. B., Vesterby, A. and West, M. J. (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS, 96, 379-394.
18. Hackney, C. M., Furness, D. N. and Steyger, P. S. (1992) Structural abnormalities in inner hair cells following kanamycin-induced outer hair cell loss. In Dallos, P., Geisler, C. D., Matthews, J. W., Ruggero, M. and Steele, C. R. (eds), Mechanics and Biophysics of Hearing. Plenum Press, New York, pp. 10-17.
19. Hackney, C. M., Osen, K. K., Ottersen, O. P., Storm-Mathisen, J. and Manjaly, G. (1996) Immunocytochemical evidence that glutamate is a neurotransmitter in the cochlear nerve: a quantitative study in the guinea-pig anteroventral cochlear nucleus. Eur. J. Neurosci., 8, 79-91.
20. Jenison, G. L., Bobbin, R. P. and Thalmann, R. (1985) Potassium-induced release of endogenous amino acids in the guinea pig cochlea. J. Neurochem., 44, 1845-1853.
21. Juiz, J. M., Rueda, J., Merchan, J. A. and Sala, M. L. (1989) The effects of kainic acid on the cochlear ganglion of the rat. Hearing Res., 40, 65-74.
22. Kanai, Y., Smith, C. P. and Hediger, M. A. (1993) The elusive transporters with a high affinity for glutamate. Trends Neurosci., 16, 365-370.
23. Kimura, R. S., Bongiorno, C. L. and Iverson, N. A. (1987) Synapses and ephapses in the spiral ganglion. Acta Otolaryngol., Suppl., 438, 1-18.
24. Kuriyama, H., Jenkins, O. and Altschuler, R. A. (1994) Immunocytochemical localization of AMPA selective glutamate receptor subunits in the rat cochlea. Hearing Res., 80, 233-240.
25. Lehre, K. P., Levy, L. M., Ottersen, O. P., Storm-Mathisen, J. and Danbolt, N. C. (1995) Differential expression of two glial glutamate transporters in the rat brain: quantitative and immunocytochemical observations. J. Neurosci., 15, 1835-1853.
26. Levy, L. M., Lehre, K. P., Walaas, S. I., Storm-Mathisen, J. and Danbolt, N. C. (1995) Down-regulation of glial glutamate transporters after glutamatergic denervation in the rat brain. Eur. J. Neurosci., 7, 2036-2041.
27. Li, H.-S., Niedzielski, A. S., Beisel, K. W., Hiel, H., Wenthold, R. J. and Morley, B. J. (1994) Identification of a glutamate/aspartate transporter in the rat cochlea. Hearing Res., 78, 235-242.
28. Liberman, M. C. (1978) Auditory nerve responses from cats raised in low noise chamber. J. Acoust. Soc. Am., 63, 442-445.
29. Lorente de Nó, R. (1933) Anatomy of the VIIIth nerve: III. General plan of the structure of the primary cochlear nuclei. Laryngoscope, 43, 327-350.
30. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
31. Matsubara, A., Laake, J. H., Davanger, S., Usami, S. and Ottersen, O. P. (1996) Organization of AMPA receptor subunits at a glutamate synapse: a quantitative immunogold analysis of hair cell synapses in the rat organ of Corti. J. Neurosci., 16, 4457-4467.
32. Melamed, B., Norris, C., Bryant, G. and Guth, P. S. (1982) Amino acid content of guinea pig perilymph collected under conditions of quiet or sound stimulation. Hearing Res., 7, 13-18.
33. Nicholls, D. and Attwell, D. (1991) The release and uptake of excitatory amino acids. Trends Pharmacol. Sci., 11, 462-468.
34. Niedzielski, A. S. and Wenthold, R. J. (1995) Expression of AMPA, kainate, and NMDA receptor subunits in cochlear and vestibular ganglia. J. Neurosci., 15, 2338-2353.
35. Parpura, V., Basarsky, T. A., Liu, F., Jeftinija, S. D. and Haydon, P. G. (1994) Glutamate-mediated astrocyte neuron signalling. Nature, 369, 744-747.
36. Parpura, V., Liu, F., Jeftinija, K. V., Haydon, P. G. and Jeftinija, S. D. (1995) Neuroligand-evoked calcium-dependent release of excitatory amino acids from Schwann cells. J. Neurosci., 15, 5831-5839.
37. Petralia, R. S., Wang, Y. X., Niedzielski, A. S. and Wenthold, R. J. (1996) The metabotropic glutamate receptors, mGluR2 and mGluR3, show unique postsynaptic, presynaptic and glial localizations. Neuroscience, 71, 949-976.
38. Puel, J.-L., Pujol, R., Ladrech, S. and Eybalin, M. (1991) α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) electrophysiological and neurotoxic effects in the cochlea. Neuroscience, 45, 63-72.
39. Puel, J.-L., Pujol, R., Tribillac, F., Ladrech, S. and Eybalin, M. (1994) Excitatory amino acid antagonists protect cochlear auditory neurons from excitotoxicity. J. Comp. Neurol., 341, 241-256.
40. Pujol, R. (1992) Neuropharmacology of the cochlea and tinnitus. In Aran J. M. and Dauman, R. (eds), Proceedings of the Fourth International Tinnitus Seminar. Kugler Publications, Amsterdam, pp. 103-107.
41. Pujol, R., Lenoir, M., Robertson, D., Eybalin, M. and Johnstone, B. M. (1985) Kainic acid selectively alters auditory dendrites connected with cochlear inner hair cells. Hearing Res., 18, 145-151.
42. Robertson, D. (1983) Functional significance of dendritic swelling after loud sounds in the guinea pig cochlea. Hearing Res., 9, 263-278.
43. Schwartz, I. R. and Ryan, A. F. (1986) Uptake of amino acids in the gerbil cochlea. In Altschuler, R. A., Hoffmann, D. W. and Bobbin, R. P. (eds), Neurobiology of Hearing: The Cochlea. Raven Press, New York, pp. 173-190.
44. Schwartz, I. R. and Ryan, A. F. (1990) Autoradiographic studies of selective amino acid uptake by neural and non-neural elements in the cochlea. J. Electron Microsc. Tech., 15, 225-244.
45. Spoendlin, H. (1972) Innervation densities of the cochlea. Acta Otolaryngol., 73, 235-248.
46. +-dependent glutamate/aspartate transporter from rat brain. Proc. Natl. Acad. Sci. USA, 89, 10955-10959.
47. Takumi, Y., Matsubara, A., Danbolt, N. C., Laake, J. H., Storm-Mathisen, J., Usami S., Shinkawa, H. and Ottersen, O. P. (1997) Discrete cellular and subcellular localization of glutamine synthetase and the glutamate transporter GLAST in the rat vestibular endorgan. Neuroscience, 79, 1137-1144.
48. Torp, R., Danbolt, N. C., Babaie, E., Bjørås, M., Seeberg, E., Storm-Mathisen, J. and Ottersen, O. P. (1994) Differential expression of two glial glutamate transporters in the rat brain: an in situ hybridization study. Eur. J. Neurosci., 6, 936-942.
49. Trotti, D., Volterra, A., Lehre, K. P., Rossi, D., Gjesdal, O., Racagni, G. and Danbolt, N. C. (1995) Arachidonic acid inhibits a purified and reconstituted glutamate transporter directly from the water phase and not via the phospholipid membrane. J. Biol. Chem., 270, 9890-9895.
50. Usami, S. and Ottersen, O. P. (1996) Aspartate is enriched in sensory cells and subpopulations of non-neuronal cells in the guinea-pig inner ear: a quantitative immunoelectron microscopic analysis. Brain Res., 742, 43-49.
51. Usami, S., Osen, K. K., Zhang, N. and Ottersen, O. P. (1992) Distribution of glutamate-like and glutamine-like immunoreactivities in the rat organ of Corti: a light and semiquantitative electron microscopic analysis with a note on the localization of aspartate. Exp. Brain Res., 91, 1-11.