Evidence of non-coccoid viable but non-culturable Campylobacter jejuni cells in microcosm water by direct viable count, CTC-DAPI double staining, and scanning electron microscopy

Food Microbiology

Volumn 15, Issue 5, 1998, Pages 539-550

  Federighi, M ^a   Tholozan, J L ^b   Cappelier, J M ^a   Tissier, J P ^b   Jouve, J L ^a  

^a LUNAM UNIVERSITÉ   (France)

^b CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIUM CULTURE; CELL ACTIVITY; MICROCOSM WATER; PH; SCANNING ELECTRON MICROSCOPY; SUSPENSION CELL CULTURE;

CAMPYLOBACTER JEJUNI;

EID: 0032191864     PISSN: 07400020     EISSN: None     Source Type: Journal    
DOI: 10.1006/fmic.1998.0181     Document Type: Article

References (33)

1. Beumer, R. R., De Vries, J. and Rombouts, F. M. (1992) Campylobacter jejuni non-culturable coccoid cells. Int. J. Food Microbiol. 15, 153-163.
2. Boucher, S. N., Slater, E. R., Chaberlain, A. H. L. and Adams, M. R. (1994) Production and viability of coccoid forms of Campylobacter jejuni. J. Appl. Bacteriol. 77, 303-307.
3. Bovill, R. A., Shallcross, J. A. and Mackey, B. M. (1994) Comparison of the fluorescent redox dye 5-cyano-2,3-ditolyltetrazolium chloride with p-iodonitrotetrazolium violet to detect metabolic activity in heat-stressed Listeria monocytogenes cells. J. Appl. Bacteriol. 77, 353-358.
4. Cappelier, J. M. and Federighi, M. (1998) Mise en évidence de l'état viable non cultivable chez Campylobacter jejuni. Rev. Méd. Vét. 149, 4, 319-326.
5. Cappelier, J. M., Lazaro, B., Rossero, A., Fernandez-Astorga, A. and Federighi, M. (1997) Double staining (CTC-DAPI) for detection and enumeration of viable but non-culturable Campylobacter jejuni cells. Vet. Res. 28, 547-555.
6. Coallier, J., Prévost, M. and Rompré, A. (1994) The optimization and application of two direct viable count methods for bacteria in distributed drinking water. Can. J. Microbiol. 40, 830-836.
7. Colwell, R. R., Brayton, P. R., Grimes, D. J., Roszak, D. B., Hug, S. A. and Palmer, L. M. (1985) Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Biotechnol. 3, 817-820.
8. Colwell, R. R., Brayton, P. R., Herrington, D., Tall, B., Huq, A. and Levine, M. M. (1996) Viable but non-culturable Vibrio cholerae 01 revert to a cultivable state in the human intestine. World J. Microbiol. Biotechnol. 12, 28-31.
9. Hazeleger, W. C., Arkenteijn, C., Toorop-Bouma, A. and Beumer, R. R. (1994) Detection of the coccoid form of Campylobacter jejuni in chicken products with the use of the polymerase chain reaction. Int. J. Food Microbiol. 24, 273-281.
10. Hazeleger, W. C., Janse, J. D., Koenraad, P. M. F. J., Beumer, R. R., Rombouts, F. M. and Abee, T. (1995) Temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of Campylobacter jejuni. Appl. Environ. Microbiol. 61, 2713-2719.
11. Hussong, D., Colwell, R. R., O'Brien, M., Weiss, E., Pearson, A. D., Weiner, R. M. and Burge, W. D. (1987) Viable Legionella pneumophila not detected by culture on agar media. Biotechnol. 5, 947-950.
12. Jones, D. M., Sutcliffe, E. M. and Curry, A. (1991) Recovery of viable but non-culturable Campylobacter jejuni. J. Gen. Microbiol. 137, 2477-2482.
13. Kobs, M. (1995) A procedure for preparing microscopic organisms for SEM. Eur. Microscopy Anal. 35, 25.
14. Kogure, K, Sindu, V. and Taga, N. (1979) A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol. 25, 415-420.
15. Kusters, J. G., Gerrits, M. M., Van Strijp, J. A. G. and Vandenbroucke-Grauls, C. M. J. E. (1997) Coccoid forms of Helicobacter pylori are the morphological manifestation of cell death. Infect. Immun. 65, 3672-3679.
16. Medema, G. J., Schets, F. M., Van de Giessen, A. W. and Havelaar, A. H. (1992) Lack of colonization of 1-day-old chicks by viable, non-culturable Campylobacter jejuni. J. Appl. Bacteriol. 72, 512-516.
17. Notermans, S. and Van de Giessen, A. W. (1993) Foodborne disease in the 1980s and 1990s. Food Control 4, 122-124.
18. Rahman, I., Shahamat, M., Kirchman, P. A., Russek-Cohen, E. and Colwell R. R. (1994) Methionine uptake and cytopathogenicity of viable but non-culturable Shigella dysenteriae type 1. Appl. Environ. Microbial. 60, 3573-3578.
19. Ravel, J., Knight, I. T., Monohan, C. E., Hill, R. T. and Colwell, R. R. (1995) Temperature-induced recovery of Vibrio cholerae from the viable but nonculturable state: growth or resuscitation? Microbiology 141, 377-383.
20. Rodriguez, G. G., Philipps, D., Ishigmo, K. and Ridgway, H. F. (1992) Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58, 1801-1808.
21. Rollins, D. M. and Colwell, R. R. (1986) Viable but non-culturable stage of Campylobacter jejuni and its role in survival in natural aquatic environment. Appl. Environ. Microbiol. 58, 1801-1808.
22. Roszak, D. B., Grimes, D. J. and Colwell, R. R. (1984) Viable but non-recoverable stage of Salmonella enteritidis in aquatic systems. Can. J. Microbiol. 30, 334-338.
23. Saha, S. K., Saha, S. and Sanyal, S. C. (1991) Recovery of injured Campylobacter jejuni cells after animal passage. Appl. Environ. Microbiol. 57, 3388-3389.
24. Schaule, G., Flemming, H. C. and Ridgway, H. F, (1993) Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl. Environ. Microbiol. 59, 3850-3857.
25. Severin, E., Stellmach, J. and Nachtigal, H. M. (1985) Fluorimetric assay of redox activity in cells. Anal. Chem. Acta 170, 341-346.
26. Skirrow, M. B. (1990) Foodborne illness: Campylobacter. Lancet 336, 921-923.
27. Smibert, M. (1984) Genus Campylobacter. In Bergey's Manual of Systematic Bacteriology, Vol. 1 (Eds N. R. Krieg and J. G. Holt) pp. 111-118. London, Williams and Wilkins.
28. Smith, J. J., Howington, J. P. and Gordon, A. M. (1994) Survival physiological response and recovery of enteric bacteria exposed to a polar marine environment. Appl. Environ. Microbiol. 60, 2977-2984.
29. Stern, N. J., Jones, D. M., Wesley, I. V. and Rollins, D. M. (1994) Colonization of chicks by non-culturable Campylobacter spp. Lett. Appl. Microbiol. 18, 333-336.
30. Taylor, D. N. (1992) Campylobacter infections in developing countries. In Campylobacter jejuni Current Topics and Future Trends (Eds I. Nachamkin, M. J. Blaser and L. S. Tompkins) pp. 20-30. Washington DC, American Society of Microbiology.
31. Van de Giessen, A. W., Heuvelman, C. J., Abee, T. and Hazeleger, W. C. (1996) Experimental studies on the infectivity of non-culturable forms of Campylobacter spp. in chicks and mice. Epidemiol. Infect. 117, 463-470.
32. Xu, H. S., Roberts, N., Singleton, F. L., Attwell, R. W., Grimes, D. J. and Colwell, R. R. (1982) Survival and viability of non-culturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microbiol. Ecol. 8, 313-323.
33. Zimmerman, R., Iturriaga, R. and Becker-Birck, J. (1978) Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl. Environ. Microbiol. 36, 926-939.