Double staining (CTC-DAPI) for detection and enumeration of viable but non-culturable Campylobacter jejuni cells

Veterinary Research

Volumn 28, Issue 6, 1997, Pages 547-555

  Cappelier, J M ^a   Lazaro, B ^b   Rossero, A ^a   Fernandez Astorga, A ^b   Federighi, M ^a  

^a CEMAGREF   (France)

^b UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

Author keywords

Campylobacter jejuni; Double staining; Starvation; Viable but non culturable

Indexed keywords

CAMPYLOBACTER JEJUNI;

4',6 DIAMIDINO 2 PHENYLINDOLE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FLUORESCENT DYE; INDOLE DERIVATIVE; TETRAZOLIUM;

ANIMAL; ANIMAL DISEASE; ARTICLE; BACTERIAL COUNT; CAMPYLOBACTER JEJUNI; CELL CULTURE; CLASSIFICATION; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; OXIDATION REDUCTION REACTION; TIME;

ANIMALS; CAMPYLOBACTER JEJUNI; CELLS, CULTURED; COLONY COUNT, MICROBIAL; FLUORESCENT DYES; INDICATORS AND REAGENTS; INDOLES; OXIDATION-REDUCTION; TETRAZOLIUM SALTS; TIME FACTORS;

EID: 0031261547     PISSN: 09284249     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Beumer RR, De Vries J, Rombouts FM (1992) Campylobacter jejuni non-culturable coccoid cells. Int J Food Microbiol 15, 153-163
2. Boucher SN, Slater ER, Chaberlain AHL, Adams MR (1994) Production and viability of coccoid forms of Campylobacter jejuni. J Appl Bacteriol 77, 303-307
3. Bovill RA, Shallcross JA, Mackey BM (1994) Comparison of the fluorescent redox dye 5-cyano-2,3-ditolyltetrazolium chloride with p-iodonitrotetrazolium violet to detect metabolic activity in heat-stressed Listeria monocytogenes cells. J Appl Bacteriol 77, 353-358
4. Coallier J, Prévost M, Rompré A (1994) The optimization and application of two direct viable count methods for bacteria in distributed drinking water. Can J Microbiol 40, 830-836
5. Colwell RR, Brayton PR, Grimes DJ, Roszak DB, Hug SA, Palmer LM (1985) Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Biotechnology 3, 817-820
6. Huang CT, Yu FP, McFeters GA, Stewart PS (1995) Nonuniform spatial patterns of respiratory activity within biofilms during disinfection. Appl Environ Microbiol 61, 2252-2256
7. Hussong D, Colwell RR, O'Brien M, Weiss E, Pearson AD, Weiner RM, Burge WD (1987) Viable Legionella pneumophilla not detected by culture on agar media. Biotechnology 5, 947-950
8. Jones DM, Sutcliffe EM, Curry A (1991) Recovery of viable but non-culturable Campylobacter jejuni. J Gen Microbiol 137, 2477-2482
9. Kogure K, Sindu V, Taga N (1979) A tentative direct microscopic method for counting living marine bacteria. Can J Microbiol 25, 415-420
10. Medema GJ, Schets FM, Van de Giessen AW, Havelaar AH (1992) Lack of colonization of 1-day-old chicks by viable, non-culturable Campylobacter jejuni. J Appl Bacteriol 72, 512-516
11. Notermans S, Hoogenboom-Verdegaal (1992) Existing and emerging foodborne diseases. Int J Food Microbiol 15, 197-205
12. Pyle BH, Broadaway SC, McFeters GA (1995) A rapid, direct method for enumerating respiring enterohaemorrhagic Escherichia coli O157:H7 in water, Appl Environ Microbiol 61, 2614-2619
13. Rahman I, Shahamat M, Kirchman PA, Russek-Cohen E, Colwell RR (1994) Methionine uptake and cytopathogenicity of viable but non-culturable Shigella dysenteriae type 1. Appl Environ Microbiol 60, 3573-3578
14. Ravel J, Knight IT, Monohan CE, Hill RT, Colwell RR (1995) Temperature-induced recovery of Vibrio cholerae from the viable but nonculturable state: growth or resuscitation? Microbiology 141, 377-383
15. Rodriguez GG, Philipps D, Ishigmo K, Ridgway HF (1992) Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl Environ Microbiol 58, 1801-1808
16. Rollins DM, Colwell RR (1986) Viable but non-culturable stage of Campylobacter jejuni and its role in survival in natural aquatic environment. Appl Environ Microbiol 58, 531-538
17. Roszak DB, Grimes DJ, Colwell RR (1984) Viable but non-recoverable stage of Salmonella enteritidis in aquatic systems. Can J Microbiol 30, 334-338
18. Roszak DB, Colwell RR (1987) Survival strategies of bacteria in the natural environment. Microbiol Rev 51, 365-379
19. Schaule G, Flemming HC, Ridgway HF (1993) Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl Environ Microbiol 59, 3850-3857
20. Severin E, Stellmach J, Nachtigal HM (1985) Fluorimetric assay of redox activity in cells. Anal Chim Acta, 170, 341-346
21. Skirrow MB (1990) Foodborne illness: Campylobacter. Lancet 336, 921-923
22. Smith JJ, Howington JP, Gordon AM (1994) Survival physiological response and recovery of enteric bacteria exposed to a polar marine environment. Appl Environ Microbiol 60, 2977-2984
23. Stellmach J, Severin E (1987) A fluorescent redox dye: influence of several substrates and electron carriers on the tetrazolium salt formazan reaction of Ehrlich ascites tumor cells. Histochem J 19, 21-26
24. Stern NJ, Jones DM, Wesley IV, Rollins DM (1994) Colonization of chicks by non-culturable Campylobacter spp. Lett Appl Microbiol 18, 333-336
25. Taylor DN (1992) Campylobacter infections in developing countries. In: Campylobacter jejuni Current Topics and Future Trends (I Nachamkin, MJ Blaser, LS Tompkins, eds), American Society of Microbiology, Washington DC, 20-30
26. Walsh S, Lappin-Scott HM, Stockdale H, Herbert BN (1995) An assessment of the metabolic activity of starved and vegetative bacteria using two redox dyes. J Microbiol Meth 24, 1-9
27. Xu HS, Roberts N, Singleton FL, Attwell RW, Grimes DJ, Colwell RR (1982) Survival and viability of non-culturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microbial Ecol 8, 313-323
28. Yu W, Dodds WK, Banks MK, Shalsky J, Strauss EA (1995) Optimal staining and sample storage time of direct microscopic enumeration of total and active bacteria in soil with two fluorescent dyes. Appl Environ Microbiol 61, 3367-3372
29. Zimmerman R, Iturriaga R, Becker-Birck J (1978) Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl Environ Microbiol 36, 926-939