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of special interest. The authors fused β-gal to the minus-end-directed microtubule motor domain of the Drosophila nod protein. Surprisingly, nod - β-gal is targed to dendrites, suggesting that dendritic microtubule polarity is reversed from that in axons. Nod - β and kinesen - β gal, which is targeted to axon terminals [19], comprise a two-reporter system for distinguishing axonal processes from dendritic ones.
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of special interest Clark IE, Jan LY, Jan YN. Reciprocal localization of nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle. Development. 124:1997;461-470 The authors fused β-gal to the minus-end-directed microtubule motor domain of the Drosophila nod protein. Surprisingly, nod - β-gal is targed to dendrites, suggesting that dendritic microtubule polarity is reversed from that in axons. Nod - β and kinesen - β gal, which is targeted to axon terminals [19], comprise a two-reporter system for distinguishing axonal processes from dendritic ones.
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Clark, I.E.1
Jan, L.Y.2
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of outstanding interest. The authors labeled P2-expressing olfactory neurons with tau - β gal and show that mutations in the P2 odorant receptor cause the axons of these neurons to wander rather than project to specific glomeruli in the olfactory bulb. This result, together with evidence of specific receptor swaps, provides evidence that odorant receptors plays instructive roles in olfactory neuron targeting. See also [24], in which tau - β-gal was first used to demonstrate that the P2 neurons normally converge on specific glomeruli and that this pattern is altered in response to receptor swaps.
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of outstanding interest Wang F, Nemes A, Mendelsohn M, Axel R. Odorant receptors govern the formation of a precise topographic map. Cell. 93:1998;47-60 The authors labeled P2-expressing olfactory neurons with tau - β gal and show that mutations in the P2 odorant receptor cause the axons of these neurons to wander rather than project to specific glomeruli in the olfactory bulb. This result, together with evidence of specific receptor swaps, provides evidence that odorant receptors plays instructive roles in olfactory neuron targeting. See also [24], in which tau - β-gal was first used to demonstrate that the P2 neurons normally converge on specific glomeruli and that this pattern is altered in response to receptor swaps.
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Wang, F.1
Nemes, A.2
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Axel, R.4
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26
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The Drosophila islet gene governs axon pathfinding and neurotransmitter identity
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of special interest. In this study, both tau - β-gal and tau - myc were used simultaneously to visualize the projections of two discrete populations of Drosophila neurons that express different LIM-homeobox genes. Double-labeling of embryos carrying both islet - tau - myc and apterous - tau - lacZ promoter fusion transgenes clearly demonstrate not only that the two populations of neurons are non-overlapping, but also that they project axons along different pathways.
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of special interest Thor S, Thomas JB. The Drosophila islet gene governs axon pathfinding and neurotransmitter identity. Neuron. 18:1997;397-409 In this study, both tau - β-gal and tau - myc were used simultaneously to visualize the projections of two discrete populations of Drosophila neurons that express different LIM-homeobox genes. Double-labeling of embryos carrying both islet - tau - myc and apterous - tau - lacZ promoter fusion transgenes clearly demonstrate not only that the two populations of neurons are non-overlapping, but also that they project axons along different pathways.
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(1997)
Neuron
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Thor, S.1
Thomas, J.B.2
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27
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Evan, G.I.1
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Ramsay, G.3
Bishop, J.M.4
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28
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Runt determines cell fates in the Drosophila embryonic CNS
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of special interest. The authors used tau - GFP to show that those neurons generated by lineages misexpressing the Drosophila runt gene extend axons in a similar fashion to neurons normally derived from runt-expressing lineages. This result supports the notion that runt specifies cell fates within the developing CNS.
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of special interest Dormand E-L, Brand AH. Runt determines cell fates in the Drosophila embryonic CNS. Development. 125:1998;1659-1667 The authors used tau - GFP to show that those neurons generated by lineages misexpressing the Drosophila runt gene extend axons in a similar fashion to neurons normally derived from runt-expressing lineages. This result supports the notion that runt specifies cell fates within the developing CNS.
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(1998)
Development
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Dormand E-L1
Brand, A.H.2
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Chalfie, M.1
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Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 111:1992;229-233.
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Liu YC, Chapman ER, Storm DR. Targeting of neuromodulin (GAP-43) fusion proteins to growth cones in cultured rat embryonic neurons. Neuron. 6:1991;411-420.
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Neuron
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Moriyoshi, K.1
Richards, L.J.2
Akazawa, C.3
O'Leary, D.D.4
Nakanishi, S.5
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33
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GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila
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of special interest. GFP has recently been fused to a number of proteins to visualize their subcellular localization. Some of these functions are good markers for neuronal processes. In this study, GFP was fused to moesin, a component of the actin cytoskeleton. GFP - moesin is a versatile, general marker of dynamic cell behavior and may prove to be a useful label for developing axons and growth cones.
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of special interest Edwards KA, Demsky M, Montague RA, Weymouth N, Kiebart DP. GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. Dev Biol. 191:1997;103-117 GFP has recently been fused to a number of proteins to visualize their subcellular localization. Some of these functions are good markers for neuronal processes. In this study, GFP was fused to moesin, a component of the actin cytoskeleton. GFP - moesin is a versatile, general marker of dynamic cell behavior and may prove to be a useful label for developing axons and growth cones.
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Dev Biol
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Edwards, K.A.1
Demsky, M.2
Montague, R.A.3
Weymouth, N.4
Kiebart, D.P.5
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34
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Lundgren SE, Callahan CA, Thor S, Thomas JB. Control of neuronal pathway selection by the Drosophila LIM homeodomain gene apterous. Development. 121:1995;1769-1773.
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Development
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Lundgren, S.E.1
Callahan, C.A.2
Thor, S.3
Thomas, J.B.4
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35
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0031952106
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Dosage-sensitive and complementary functions of Roundabout and Commissureless control axons crossing of the CNS midline
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of special interest. The authors used a trasgene consisting of the apterous neuronal enhancer driving tau - lacZ [34] to examine the pathfinding behavior of the apterous neurons in roundabout (robo) mutant embryos. These neurons normally project ipsilaterally in the Drosophila CNS; however, in robo mutants, all of the apterous neurons abnormally cross the midline. A milder phenotype is seen in robo heterozygous embryos, demonstrating that robo controls midline crossing of axons in a dosage-sensitive manner.
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of special interest Kidd T, Russell C, Goodman CS, Tear G. Dosage-sensitive and complementary functions of Roundabout and Commissureless control axons crossing of the CNS midline. Neuron. 20:1998;25-33 The authors used a trasgene consisting of the apterous neuronal enhancer driving tau - lacZ [34] to examine the pathfinding behavior of the apterous neurons in roundabout (robo) mutant embryos. These neurons normally project ipsilaterally in the Drosophila CNS; however, in robo mutants, all of the apterous neurons abnormally cross the midline. A milder phenotype is seen in robo heterozygous embryos, demonstrating that robo controls midline crossing of axons in a dosage-sensitive manner.
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(1998)
Neuron
, vol.20
, pp. 25-33
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Kidd, T.1
Russell, C.2
Goodman, C.S.3
Tear, G.4
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36
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0028998860
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Control of neuronal pathway selection by a Drosophila receptor protein-tyrosine kinase family member
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Callahan CA, Muralidhar MG, Lundgren SE, Scully AL, Thomas JB. Control of neuronal pathway selection by a Drosophila receptor protein-tyrosine kinase family member. Nature. 376:1995;171-174.
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Nature
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, pp. 171-174
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Callahan, C.A.1
Muralidhar, M.G.2
Lundgren, S.E.3
Scully, A.L.4
Thomas, J.B.5
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37
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Brand AH, Perrimon N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 118:1993;401-415.
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Development
, vol.118
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Brand, A.H.1
Perrimon, N.2
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38
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0030776763
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Targeted neuronal ablation: The role of pioneer neurons in guidance and fasciculation in the CNS of Drosophila
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of special interest. The authors capitalized on a set of fly enhancer trap lines that express GAL4 in small subsets of early-developing pioneer neurons [37]. In order to visualize the projections of the GAL4-expressing neurons, a UAS - tau - lacZ transgene was crossed into each of the GAL4 lines. GAL4 binds to the upstream-activating sequence (UAS), resulting in transactivation of tau - lacZ only in those neurons expressing GAL4. The roles these pioneer neurons play in establishing pathways within the developing CNS was determined by ablating them with a UAS - ricin transgene.
-
of special interest Hidalgo A, Brand AH. Targeted neuronal ablation: the role of pioneer neurons in guidance and fasciculation in the CNS of Drosophila. Development. 124:1997;3253-3262 The authors capitalized on a set of fly enhancer trap lines that express GAL4 in small subsets of early-developing pioneer neurons [37]. In order to visualize the projections of the GAL4-expressing neurons, a UAS - tau - lacZ transgene was crossed into each of the GAL4 lines. GAL4 binds to the upstream-activating sequence (UAS), resulting in transactivation of tau - lacZ only in those neurons expressing GAL4. The roles these pioneer neurons play in establishing pathways within the developing CNS was determined by ablating them with a UAS - ricin transgene.
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(1997)
Development
, vol.124
, pp. 3253-3262
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Hidalgo, A.1
Brand, A.H.2
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39
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Francis JW, Hosler BA, Brown RH Jr, Fishman PS. CuZn superoxide dismutase (SOD-1):tetanus toxin fragment C hybrid protein for targeted delivery of SOD-1 to neuronal cells. J Biol Chem. 270:1995;15434-15442.
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of outstanding interest. The authors show that a purified recombinant protein consisting of β-gal fused to the tetanus toxin C fragment (TTC) is transported retrogradely and transynaptically within the CNS. This study raises the possibility that the TTC - β-gal fusion reporter, as well as fusions of TTC to other reporters, could be expressed by subsets of cells in transgenic animals and transynaptically transported to label specific classes of interconnected neurons.
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of outstanding interest Coen L, Osta R, Maury M, Brulet P. Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system. Proc Natl Acad Sci USA. 94:1997;9400-9405 The authors show that a purified recombinant protein consisting of β-gal fused to the tetanus toxin C fragment (TTC) is transported retrogradely and transynaptically within the CNS. This study raises the possibility that the TTC - β-gal fusion reporter, as well as fusions of TTC to other reporters, could be expressed by subsets of cells in transgenic animals and transynaptically transported to label specific classes of interconnected neurons.
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Proc Natl Acad Sci USA
, vol.94
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Coen, L.1
Osta, R.2
Maury, M.3
Brulet, P.4
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