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Volumn 8, Issue 5, 1998, Pages 582-586

Tracing axons

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; GENE PRODUCT; GREEN FLUORESCENT PROTEIN; HOMEODOMAIN PROTEIN; HYBRID PROTEIN; KINESIN; TAU PROTEIN; TETANUS TOXIN; TRACER;

EID: 0032191850     PISSN: 09594388     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-4388(98)80084-6     Document Type: Article
Times cited : (31)

References (42)
  • 1
    • 0000866323 scopus 로고
    • Sulla sostanza grigia del cervello
    • On the grey matter of the brain.
    • On the grey matter of the brain. Golgi C. Sulla sostanza grigia del cervello. Gaz Med Ital Lombardia. 6:1873;244-246.
    • (1873) Gaz Med Ital Lombardia , vol.6 , pp. 244-246
    • Golgi, C.1
  • 2
    • 84907127493 scopus 로고
    • Silver impregnation of degenerating axons in the central nervous system: A modified technique
    • Nauta WJH, Gygax PA. Silver impregnation of degenerating axons in the central nervous system: a modified technique. Stain Technol. 29:1954;91-93.
    • (1954) Stain Technol , vol.29 , pp. 91-93
    • Nauta, W.J.H.1    Gygax, P.A.2
  • 3
    • 0018604888 scopus 로고
    • An approach to tracing neuron networks in the cerebral cortex and basal ganglia. Combination of Golgi staining, retrograde transport of horseradish peroxidase and anterograde degeneration of synaptic boutons in the same material
    • Somogyi P, Hodgson AJ, Smith AD. An approach to tracing neuron networks in the cerebral cortex and basal ganglia. Combination of Golgi staining, retrograde transport of horseradish peroxidase and anterograde degeneration of synaptic boutons in the same material. Neuroscience. 4:1979;1805-1852.
    • (1979) Neuroscience , vol.4 , pp. 1805-1852
    • Somogyi, P.1    Hodgson, A.J.2    Smith, A.D.3
  • 5
    • 0020468968 scopus 로고
    • Monoclonal antibodies distinguish antigenically discrete neuronal types in the vertebrate central nervous system
    • McKay RD, Hockfield SJ. Monoclonal antibodies distinguish antigenically discrete neuronal types in the vertebrate central nervous system. Proc Natl Acad Sci USA. 79:1982;6747-6751.
    • (1982) Proc Natl Acad Sci USA , vol.79 , pp. 6747-6751
    • McKay, R.D.1    Hockfield, S.J.2
  • 6
    • 0021346195 scopus 로고
    • Neuronal development in the Drosophila retina: Monoclonal antibodies as molecular probes
    • Zipursky S, Venkatesh T, Teplow D, Benzer S. Neuronal development in the Drosophila retina: monoclonal antibodies as molecular probes. Cell. 36:1984;15-26.
    • (1984) Cell , vol.36 , pp. 15-26
    • Zipursky, S.1    Venkatesh, T.2    Teplow, D.3    Benzer, S.4
  • 7
    • 0014402973 scopus 로고
    • Neuronal geometry: Determination with a technique of intracellular dye injection
    • Stretton AO, Kravitz EA. Neuronal geometry: determination with a technique of intracellular dye injection. Science. 162:1968;132-134.
    • (1968) Science , vol.162 , pp. 132-134
    • Stretton, A.O.1    Kravitz, E.A.2
  • 8
    • 0015526863 scopus 로고
    • Branching of central neurons: Intracellular cobalt injection for light and electron microscopy
    • Pitman RM, Tweedle CD, Cohem MJ. Branching of central neurons: intracellular cobalt injection for light and electron microscopy. Science. 176:1972;412-414.
    • (1972) Science , vol.176 , pp. 412-414
    • Pitman, R.M.1    Tweedle, C.D.2    Cohem, M.J.3
  • 9
    • 0010365985 scopus 로고
    • Intracellular horseradish peroxidase injections for tracing neural connections
    • Mesulam M.M. New York: John Wiley and Sons
    • Bishop GA, King JS. Intracellular horseradish peroxidase injections for tracing neural connections. Mesulam MM. Tracing Neural Connections with Horseradish Peroxidase. 1982;185-247 John Wiley and Sons, New York.
    • (1982) Tracing Neural Connections with Horseradish Peroxidase , pp. 185-247
    • Bishop, G.A.1    King, J.S.2
  • 10
    • 0017992761 scopus 로고
    • Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer
    • Stewart WW. Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell. 14:1978;741-759.
    • (1978) Cell , vol.14 , pp. 741-759
    • Stewart, W.W.1
  • 11
    • 0021369741 scopus 로고
    • An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: Immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L)
    • Gerfen CR, Sawchenko PE. An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L). Brain Res. 290:1984;219-238.
    • (1984) Brain Res , vol.290 , pp. 219-238
    • Gerfen, C.R.1    Sawchenko, P.E.2
  • 12
    • 0014671663 scopus 로고
    • Electron microscopic radioautography: Identification of origin of synaptic terminals in normal nervous tissue
    • Hendrickson A. Electron microscopic radioautography: identification of origin of synaptic terminals in normal nervous tissue. Science. 165:1969;194-196.
    • (1969) Science , vol.165 , pp. 194-196
    • Hendrickson, A.1
  • 14
    • 0024415666 scopus 로고
    • Biocytin: A versatile anterograde neuroanatomical tract-tracing alternative
    • King MA, Louis PM, Hunter BE, Walker DW. Biocytin: a versatile anterograde neuroanatomical tract-tracing alternative. Brain Res. 497:1989;361-367.
    • (1989) Brain Res , vol.497 , pp. 361-367
    • King, M.A.1    Louis, P.M.2    Hunter, B.E.3    Walker, D.W.4
  • 15
    • 0024312004 scopus 로고
    • Dil and diO: Versatile fluorescent dyes for neuronal labelling and pathway tracing
    • Honig MG, Hume RI. Dil and diO: versatile fluorescent dyes for neuronal labelling and pathway tracing. Trends Neurosci. 12:1989;333-335.
    • (1989) Trends Neurosci , vol.12 , pp. 333-335
    • Honig, M.G.1    Hume, R.I.2
  • 16
    • 0022317468 scopus 로고
    • Control elements of the Drosophila segmentation gene fushi tarazu
    • Horimi Y, Kuroiwa A, Gehring WJ. Control elements of the Drosophila segmentation gene fushi tarazu. Cell. 43:1985;603-613.
    • (1985) Cell , vol.43 , pp. 603-613
    • Horimi, Y.1    Kuroiwa, A.2    Gehring, W.J.3
  • 17
    • 0024799201 scopus 로고
    • The mec-3 gene of Caenorhabditis elegans requires its own product for maintained expression and is expressed in three neuronal cell types
    • Way JC, Chalfie M. The mec-3 gene of Caenorhabditis elegans requires its own product for maintained expression and is expressed in three neuronal cell types. Genes Dev. 3:1989;1823-1833.
    • (1989) Genes Dev , vol.3 , pp. 1823-1833
    • Way, J.C.1    Chalfie, M.2
  • 18
    • 11944259305 scopus 로고
    • Introduction of a laZ reporter gene into the mouse int-2 locus by homologous recombination
    • Mansour SL, Thomas KR, Deng CX, Capecchi MR. Introduction of a laZ reporter gene into the mouse int-2 locus by homologous recombination. Proc Natl Acad Sci USA. 87:1990;7688-7692.
    • (1990) Proc Natl Acad Sci USA , vol.87 , pp. 7688-7692
    • Mansour, S.L.1    Thomas, K.R.2    Deng, C.X.3    Capecchi, M.R.4
  • 19
    • 0027464804 scopus 로고
    • Tracing neurons with a kinesin-β-galactosidase fusion protein
    • Giniger E, Wells W, Jan LY, Jan YN. Tracing neurons with a kinesin-β-galactosidase fusion protein. Rouxs Arch Dev Biol. 202:1993;112-122.
    • (1993) Rouxs Arch Dev Biol , vol.202 , pp. 112-122
    • Giniger, E.1    Wells, W.2    Jan, L.Y.3    Jan, Y.N.4
  • 20
    • 0029670680 scopus 로고    scopus 로고
    • Eagle, a member of the steroid receptor gene superfamily, is expressed in a subset of neuroblasts and regulates the fate of their putative progeny in the Drosophila CNS
    • Higashijima S, Shishido E, Matsuzaki M, Saigo K. eagle, a member of the steroid receptor gene superfamily, is expressed in a subset of neuroblasts and regulates the fate of their putative progeny in the Drosophila CNS. Development. 122:1996;527-536.
    • (1996) Development , vol.122 , pp. 527-536
    • Higashijima, S.1    Shishido, E.2    Matsuzaki, M.3    Saigo, K.4
  • 21
    • 0031040498 scopus 로고    scopus 로고
    • Reciprocal localization of nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle
    • of special interest. The authors fused β-gal to the minus-end-directed microtubule motor domain of the Drosophila nod protein. Surprisingly, nod - β-gal is targed to dendrites, suggesting that dendritic microtubule polarity is reversed from that in axons. Nod - β and kinesen - β gal, which is targeted to axon terminals [19], comprise a two-reporter system for distinguishing axonal processes from dendritic ones.
    • of special interest Clark IE, Jan LY, Jan YN. Reciprocal localization of nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle. Development. 124:1997;461-470 The authors fused β-gal to the minus-end-directed microtubule motor domain of the Drosophila nod protein. Surprisingly, nod - β-gal is targed to dendrites, suggesting that dendritic microtubule polarity is reversed from that in axons. Nod - β and kinesen - β gal, which is targeted to axon terminals [19], comprise a two-reporter system for distinguishing axonal processes from dendritic ones.
    • (1997) Development , vol.124 , pp. 461-470
    • Clark, I.E.1    Jan, L.Y.2    Jan, Y.N.3
  • 22
    • 0028173714 scopus 로고
    • Tau-β-galactosidase, an axon-targeted fusion protein
    • Callahan CA, Thomas JB. Tau-β-galactosidase, an axon-targeted fusion protein. Proc Natl Acad Sci USA. 91:1994;5972-5976.
    • (1994) Proc Natl Acad Sci USA , vol.91 , pp. 5972-5976
    • Callahan, C.A.1    Thomas, J.B.2
  • 23
    • 0026046950 scopus 로고
    • Tau protein binds to microtubules through a flexible array of distributed weak sites
    • Butner KA, Kirschner MW. Tau protein binds to microtubules through a flexible array of distributed weak sites. J Cell Biol. 115:1991;717-730.
    • (1991) J Cell Biol , vol.115 , pp. 717-730
    • Butner, K.A.1    Kirschner, M.W.2
  • 25
    • 0032478533 scopus 로고    scopus 로고
    • Odorant receptors govern the formation of a precise topographic map
    • of outstanding interest. The authors labeled P2-expressing olfactory neurons with tau - β gal and show that mutations in the P2 odorant receptor cause the axons of these neurons to wander rather than project to specific glomeruli in the olfactory bulb. This result, together with evidence of specific receptor swaps, provides evidence that odorant receptors plays instructive roles in olfactory neuron targeting. See also [24], in which tau - β-gal was first used to demonstrate that the P2 neurons normally converge on specific glomeruli and that this pattern is altered in response to receptor swaps.
    • of outstanding interest Wang F, Nemes A, Mendelsohn M, Axel R. Odorant receptors govern the formation of a precise topographic map. Cell. 93:1998;47-60 The authors labeled P2-expressing olfactory neurons with tau - β gal and show that mutations in the P2 odorant receptor cause the axons of these neurons to wander rather than project to specific glomeruli in the olfactory bulb. This result, together with evidence of specific receptor swaps, provides evidence that odorant receptors plays instructive roles in olfactory neuron targeting. See also [24], in which tau - β-gal was first used to demonstrate that the P2 neurons normally converge on specific glomeruli and that this pattern is altered in response to receptor swaps.
    • (1998) Cell , vol.93 , pp. 47-60
    • Wang, F.1    Nemes, A.2    Mendelsohn, M.3    Axel, R.4
  • 26
    • 0030939130 scopus 로고    scopus 로고
    • The Drosophila islet gene governs axon pathfinding and neurotransmitter identity
    • of special interest. In this study, both tau - β-gal and tau - myc were used simultaneously to visualize the projections of two discrete populations of Drosophila neurons that express different LIM-homeobox genes. Double-labeling of embryos carrying both islet - tau - myc and apterous - tau - lacZ promoter fusion transgenes clearly demonstrate not only that the two populations of neurons are non-overlapping, but also that they project axons along different pathways.
    • of special interest Thor S, Thomas JB. The Drosophila islet gene governs axon pathfinding and neurotransmitter identity. Neuron. 18:1997;397-409 In this study, both tau - β-gal and tau - myc were used simultaneously to visualize the projections of two discrete populations of Drosophila neurons that express different LIM-homeobox genes. Double-labeling of embryos carrying both islet - tau - myc and apterous - tau - lacZ promoter fusion transgenes clearly demonstrate not only that the two populations of neurons are non-overlapping, but also that they project axons along different pathways.
    • (1997) Neuron , vol.18 , pp. 397-409
    • Thor, S.1    Thomas, J.B.2
  • 27
    • 0022340978 scopus 로고
    • Isolation of monoclonal antibodies specific for human c-myc protooncogene product
    • Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc protooncogene product. Mol Cell Biol. 5:1985;3610-3616.
    • (1985) Mol Cell Biol , vol.5 , pp. 3610-3616
    • Evan, G.I.1    Lewis, G.K.2    Ramsay, G.3    Bishop, J.M.4
  • 28
    • 0031831564 scopus 로고    scopus 로고
    • Runt determines cell fates in the Drosophila embryonic CNS
    • of special interest. The authors used tau - GFP to show that those neurons generated by lineages misexpressing the Drosophila runt gene extend axons in a similar fashion to neurons normally derived from runt-expressing lineages. This result supports the notion that runt specifies cell fates within the developing CNS.
    • of special interest Dormand E-L, Brand AH. Runt determines cell fates in the Drosophila embryonic CNS. Development. 125:1998;1659-1667 The authors used tau - GFP to show that those neurons generated by lineages misexpressing the Drosophila runt gene extend axons in a similar fashion to neurons normally derived from runt-expressing lineages. This result supports the notion that runt specifies cell fates within the developing CNS.
    • (1998) Development , vol.125 , pp. 1659-1667
    • Dormand E-L1    Brand, A.H.2
  • 29
  • 31
    • 0025963276 scopus 로고
    • Targeting of neuromodulin (GAP-43) fusion proteins to growth cones in cultured rat embryonic neurons
    • Liu YC, Chapman ER, Storm DR. Targeting of neuromodulin (GAP-43) fusion proteins to growth cones in cultured rat embryonic neurons. Neuron. 6:1991;411-420.
    • (1991) Neuron , vol.6 , pp. 411-420
    • Liu, Y.C.1    Chapman, E.R.2    Storm, D.R.3
  • 32
    • 0030048198 scopus 로고    scopus 로고
    • Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP
    • Moriyoshi K, Richards LJ, Akazawa C, O'Leary DD, Nakanishi S. Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP. Neuron. 16:1996;255-260.
    • (1996) Neuron , vol.16 , pp. 255-260
    • Moriyoshi, K.1    Richards, L.J.2    Akazawa, C.3    O'Leary, D.D.4    Nakanishi, S.5
  • 33
    • 0031281917 scopus 로고    scopus 로고
    • GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila
    • of special interest. GFP has recently been fused to a number of proteins to visualize their subcellular localization. Some of these functions are good markers for neuronal processes. In this study, GFP was fused to moesin, a component of the actin cytoskeleton. GFP - moesin is a versatile, general marker of dynamic cell behavior and may prove to be a useful label for developing axons and growth cones.
    • of special interest Edwards KA, Demsky M, Montague RA, Weymouth N, Kiebart DP. GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. Dev Biol. 191:1997;103-117 GFP has recently been fused to a number of proteins to visualize their subcellular localization. Some of these functions are good markers for neuronal processes. In this study, GFP was fused to moesin, a component of the actin cytoskeleton. GFP - moesin is a versatile, general marker of dynamic cell behavior and may prove to be a useful label for developing axons and growth cones.
    • (1997) Dev Biol , vol.191 , pp. 103-117
    • Edwards, K.A.1    Demsky, M.2    Montague, R.A.3    Weymouth, N.4    Kiebart, D.P.5
  • 34
    • 0029050820 scopus 로고
    • Control of neuronal pathway selection by the Drosophila LIM homeodomain gene apterous
    • Lundgren SE, Callahan CA, Thor S, Thomas JB. Control of neuronal pathway selection by the Drosophila LIM homeodomain gene apterous. Development. 121:1995;1769-1773.
    • (1995) Development , vol.121 , pp. 1769-1773
    • Lundgren, S.E.1    Callahan, C.A.2    Thor, S.3    Thomas, J.B.4
  • 35
    • 0031952106 scopus 로고    scopus 로고
    • Dosage-sensitive and complementary functions of Roundabout and Commissureless control axons crossing of the CNS midline
    • of special interest. The authors used a trasgene consisting of the apterous neuronal enhancer driving tau - lacZ [34] to examine the pathfinding behavior of the apterous neurons in roundabout (robo) mutant embryos. These neurons normally project ipsilaterally in the Drosophila CNS; however, in robo mutants, all of the apterous neurons abnormally cross the midline. A milder phenotype is seen in robo heterozygous embryos, demonstrating that robo controls midline crossing of axons in a dosage-sensitive manner.
    • of special interest Kidd T, Russell C, Goodman CS, Tear G. Dosage-sensitive and complementary functions of Roundabout and Commissureless control axons crossing of the CNS midline. Neuron. 20:1998;25-33 The authors used a trasgene consisting of the apterous neuronal enhancer driving tau - lacZ [34] to examine the pathfinding behavior of the apterous neurons in roundabout (robo) mutant embryos. These neurons normally project ipsilaterally in the Drosophila CNS; however, in robo mutants, all of the apterous neurons abnormally cross the midline. A milder phenotype is seen in robo heterozygous embryos, demonstrating that robo controls midline crossing of axons in a dosage-sensitive manner.
    • (1998) Neuron , vol.20 , pp. 25-33
    • Kidd, T.1    Russell, C.2    Goodman, C.S.3    Tear, G.4
  • 36
    • 0028998860 scopus 로고
    • Control of neuronal pathway selection by a Drosophila receptor protein-tyrosine kinase family member
    • Callahan CA, Muralidhar MG, Lundgren SE, Scully AL, Thomas JB. Control of neuronal pathway selection by a Drosophila receptor protein-tyrosine kinase family member. Nature. 376:1995;171-174.
    • (1995) Nature , vol.376 , pp. 171-174
    • Callahan, C.A.1    Muralidhar, M.G.2    Lundgren, S.E.3    Scully, A.L.4    Thomas, J.B.5
  • 37
    • 0027160708 scopus 로고
    • Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
    • Brand AH, Perrimon N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 118:1993;401-415.
    • (1993) Development , vol.118 , pp. 401-415
    • Brand, A.H.1    Perrimon, N.2
  • 38
    • 0030776763 scopus 로고    scopus 로고
    • Targeted neuronal ablation: The role of pioneer neurons in guidance and fasciculation in the CNS of Drosophila
    • of special interest. The authors capitalized on a set of fly enhancer trap lines that express GAL4 in small subsets of early-developing pioneer neurons [37]. In order to visualize the projections of the GAL4-expressing neurons, a UAS - tau - lacZ transgene was crossed into each of the GAL4 lines. GAL4 binds to the upstream-activating sequence (UAS), resulting in transactivation of tau - lacZ only in those neurons expressing GAL4. The roles these pioneer neurons play in establishing pathways within the developing CNS was determined by ablating them with a UAS - ricin transgene.
    • of special interest Hidalgo A, Brand AH. Targeted neuronal ablation: the role of pioneer neurons in guidance and fasciculation in the CNS of Drosophila. Development. 124:1997;3253-3262 The authors capitalized on a set of fly enhancer trap lines that express GAL4 in small subsets of early-developing pioneer neurons [37]. In order to visualize the projections of the GAL4-expressing neurons, a UAS - tau - lacZ transgene was crossed into each of the GAL4 lines. GAL4 binds to the upstream-activating sequence (UAS), resulting in transactivation of tau - lacZ only in those neurons expressing GAL4. The roles these pioneer neurons play in establishing pathways within the developing CNS was determined by ablating them with a UAS - ricin transgene.
    • (1997) Development , vol.124 , pp. 3253-3262
    • Hidalgo, A.1    Brand, A.H.2
  • 39
    • 0029613765 scopus 로고
    • Structure and function of tetanus and botulinum neurotoxins
    • Montecucco C, Schiavo G. Structure and function of tetanus and botulinum neurotoxins. Q Rev Biophys. 28:1995;423-472.
    • (1995) Q Rev Biophys , vol.28 , pp. 423-472
    • Montecucco, C.1    Schiavo, G.2
  • 40
    • 0025233759 scopus 로고
    • Cloning and expression of functional fragment C of tetanus toxin
    • Halpern JL, Habig WH, Neale EA, Stibitz S. Cloning and expression of functional fragment C of tetanus toxin. Infect Immun. 58:1990;1004-1009.
    • (1990) Infect Immun , vol.58 , pp. 1004-1009
    • Halpern, J.L.1    Habig, W.H.2    Neale, E.A.3    Stibitz, S.4
  • 41
    • 0029034875 scopus 로고
    • CuZn superoxide dismutase (SOD-1):tetanus toxin fragment C hybrid protein for targeted delivery of SOD-1 to neuronal cells
    • Francis JW, Hosler BA, Brown RH Jr, Fishman PS. CuZn superoxide dismutase (SOD-1):tetanus toxin fragment C hybrid protein for targeted delivery of SOD-1 to neuronal cells. J Biol Chem. 270:1995;15434-15442.
    • (1995) J Biol Chem , vol.270 , pp. 15434-15442
    • Francis, J.W.1    Hosler, B.A.2    Brown R.H., Jr.3    Fishman, P.S.4
  • 42
    • 0030740254 scopus 로고    scopus 로고
    • Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system
    • of outstanding interest. The authors show that a purified recombinant protein consisting of β-gal fused to the tetanus toxin C fragment (TTC) is transported retrogradely and transynaptically within the CNS. This study raises the possibility that the TTC - β-gal fusion reporter, as well as fusions of TTC to other reporters, could be expressed by subsets of cells in transgenic animals and transynaptically transported to label specific classes of interconnected neurons.
    • of outstanding interest Coen L, Osta R, Maury M, Brulet P. Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system. Proc Natl Acad Sci USA. 94:1997;9400-9405 The authors show that a purified recombinant protein consisting of β-gal fused to the tetanus toxin C fragment (TTC) is transported retrogradely and transynaptically within the CNS. This study raises the possibility that the TTC - β-gal fusion reporter, as well as fusions of TTC to other reporters, could be expressed by subsets of cells in transgenic animals and transynaptically transported to label specific classes of interconnected neurons.
    • (1997) Proc Natl Acad Sci USA , vol.94 , pp. 9400-9405
    • Coen, L.1    Osta, R.2    Maury, M.3    Brulet, P.4


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