RT-PCR quantitation of cytokine responses in vivo from specimens containing small numbers of cells during bioimmunotherapy

Journal of Immunological Methods

Volumn 219, Issue 1-2, 1998, Pages 169-179

  Nash, Micheal A ^a   Lenzi, Renato ^a   Platsoucas, Chris D ^b   Freedman, Ralph S ^a  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^b TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cytokines; Ovarian cancer; Semiquantitative RT PCR

Indexed keywords

COMPLEMENTARY DNA; CYTOKINE; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INTERLEUKIN 10; MESSENGER RNA;

ARTICLE; CANCER IMMUNOTHERAPY; DENSITOMETRY; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; OVARY CARCINOMA; PERITONEAL EXUDATE CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ASCITIC FLUID; CYTOKINES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; IMMUNOTHERAPY; INTERFERON TYPE II; INTERFERON-GAMMA, RECOMBINANT; INTERLEUKIN-10; INTERLEUKIN-2; OVARIAN NEOPLASMS; PERITONEAL CAVITY; PERITONEAL NEOPLASMS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0032191736     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(98)00135-5     Document Type: Article

References (18)

1. Chang A.E., Aruga A., Cameron M.J., Sondak V.K., Normolle D.P., Fox B.A., Shu S. Adoptive immunotherapy with vaccine-primed lymph node cells secondarily activated with anti-CD3 and interleukin-2. Journal of Clinical Oncology. 15:1997;796.
2. Freedman R.S., Tomasovic B., Templin S., Atkinson E.N., Kudelka A., Edwards C.L., Platsoucas C.D. Large-scale expansion in interleukin-2 of tumor-infiltrating lymphocytes from patients with ovarian carcinoma for adoptive immunotherapy. J. Immunol. Methods. 167:1994;145.
3. Freedman, R.S., Lenzi, R., Kudelka, A.P., Rosenblum, M., Platsoucas, C.D., 1998. Intraperitoneal immunotherapy of peritoneal carcinomatosis. Cytokines, Cellular and Molecular Therapy, in press.
4. Garbay B., Boue-Grabot E., Garret M. Processed pseudogenes interfere with reverse transcriptase-polymerase chain reaction controls. Anal. Biochem. 237:1996;157.
5. Gordinier M., Zhang H.Z., Kidd L., Patenia R., Levy L., Katz R.L., Nash M.A., Edwards C.L., Platsoucas C.D., Freedman R.S. Quantitative analysis of transforming growth factor-β (TGF-β) and HLA class I and II in epithelial ovarian cancer. Proc. Annu. Meet. Amer. Assoc. Cancer Res. 39:1998;A1033.
6. Kotake S., Schumacher H.R. Jr., Wilder R.L. A simple nested RT-PCR method for quantitation of the relative amounts of multiple cytokine mRNAs in small tissue samples. J. Immunol. Methods. 199:1996;193.
7. Lagoo A.S., Eldridge J.H., Lagoo-Deenadaylan S., Black C.A., Ridwan B.U., Hardy K.J., McGhee J.R., Beagley D.W. Peyers patch CD8+memory T cells secrete T helper type 1 and type 2 cytokines and provide help for immunoglobulin secretion. Eur. J. Immunol. 24:1994;3087.
8. Loercher, A., Nash, M., Kavanagh, J., Edwards, C., Platsoucas, C., Freedman, R.S., 1998. Effects of IL-10 producing monocytes on the proliferation and cytokine production of ascitic T-cells from patients with peritoneal carcinomatosis. FASEB J., A1608.
9. Melichar B., Templin S.M., Tomasovic B.J., Kudelka A.P., Kavanagh J.J., Edwards C.L., Platsoucas C.D., Freedman R.S. Effects of intraperitoneal injections of interferon-γ and interleukin-2 on peritoneal monocytes and dendritic cells. Proc. Annu. Meet. Am. Assoc. Cancer Res. 38:1997;A4239.
10. Nash, M.A., Lenzi, R., Edwards, J.J., Kavanagh, J.J., Kudelka, A.P., Verschraegen, C.F., Platsoucas, C.D., Freedman, R.S., 1998. Differential expression of cytokine transcripts in human epithelial ovarian carcinoma by solid tumor specimens, peritoneal exudate cells containing tumor, TIL-derived T-cell lines and established tumor cell lines. Clin. Exp. Immunol., in press.
11. Pisa P., Halapi E., Pisa E.K., Gerdin E., Hising C., Bucht A., Gerdin B., Kiessling R. Selective expression of interleukin 10, interferon γ and granulocyte-macrophage colony stimulating factor in ovarian cancer biopsies. Proc. Natl. Acad. Sci. U.S.A. 89:1992;7708.
12. Reyes-Engel A., Garcia-Villanova J., Deiguez-Lucena J.F., Fernandez-Arcas N., Ruiz-Galdon M. New approach to mRNA quantification: additive RT-PCR. BioTechniques. 21:1996;202.
13. Rosenberg R.A. Cancer vaccines based on the identification of genes encoding cancer regression antigens. Immunology Today. 18:1997;175.
14. Russell M.E., Wallace A.F., Hancock W.W., Sayegh M.H., Adams D.H., Sibinga N.E.S., Wyner L.R., Karnovsky M.J. Upregulation of cytokines associated with macrophage activation in the Lewis-TO-F344 rat transplantation model of chronic cardiac rejection. Transplantation. 59:1995;572.
15. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Siebert P.D., Larrick J.W. PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR. BioTechniques. 14:1993;244.
17. Spagnoli G.C., Juretic A., Schultz-Thater E., Dellabona P., Filgueira L., Hörig H., Zuber M., Garotta G., Heberer M. On the relative roles of interleukin-2 and interleukin-10 in the generation of lymphokine-activated killer cell activity. Cell. Immunol. 146:1993;391.
18. Xie W.Q., Rothblum L.I. Rapid, small-scale RNA isolation from tissue culture cells. BioTechniques. 11:1991;324.