New approach to mRNA quantification: Additive RT-PCR

BioTechniques

Volumn 21, Issue 2, 1996, Pages 202-204

  Reyes Engel, A ^a   Garcia Villanova J ^a   Dieguez Lucena, J L ^a   Fernandez Arcas N ^a   Ruiz Galdon M ^a  

^a UNIVERSITY OF MÁLAGA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; ARTICLE; DIAGNOSTIC ACCURACY; GENE EXPRESSION; LIPOPROTEIN METABOLISM; QUANTITATIVE DIAGNOSIS; RECEPTOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

EID: 0029783046     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212bm06     Document Type: Article

References (5)

1. + lymphopenia in HIV type 2-infected individuals. AIDS Res. Hum. Retroviruses 10:1031-1037.
2. Chomcznsky, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
3. ™ during HIV-1 primary infection. J. Virol. Meth. 43:177-188.
4. Matsumoto, A., M. Naito, H. Itakura, S. Ikemoto, H. Asaoka, I. Hayakawa, H. Kanamori, H. Aburatani, et al. 1990. Human macrophage scavenger receptors: primary structure, expression and localization in atherosclerotic lesions. Proc. Natl. Acad. Sci. USA 57:9133-9137.
5. Reyes-Engel, A. and J.L. Dieguez-Lucena. 1993. Direct quantification of specific mRNA using a selected biotinylated oligonucleotide by free solution capillary electrophoresis. Nucleic Acids Res. 21:759-760.