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R screening set)] as suitable biocatalyst for deprotection of sugar acylated nucleosides in neutral aqueous/organic solutions. Biocatalysts tested have varying activities and specifities for a range of different purine, acyclopurine and pyrimidine nucleosides dependent on solvent and temperature.
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14
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85038539763
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note
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8. Two centriplus-10 (10,000 MW cut off; Amicon Corp.) devices were washed with 1.0 mL 18 Mohm water to remove any residual preservatives prior to loading sample. Each centriplus-10 was loaded with the reaction mixture and spun at 6,000 rpm in an ultracentrifuge for 12 h at 4°C to dryness. An additional 1.0 mL 50 mM MOPS buffer, pH 7.0 was added to each centriplus-10 and spun at 6,000 rpm for 4h at 4°C. The ESL-001-02 biocatalyst was then recovered from the retentate and stored at 4°C in the 50 mM MOPS, pH 7.0 for reuse. The ESL-001-02 biocatalyst was reused many times (>6) in the deprotection of 2 with little or no apparent loss in activity.
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15
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85038540424
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note
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9. Slow decomposition of 1 may occur during chromatography for which a rapid purification procedure is necessary.
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16
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85038544493
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note
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4F = 286.0952; Found: 286.0955.
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17
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0020490596
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13C-NMR (Nair, V.; Young, D.A. Magnet. Res. Chem. 1987, 25, 937), it has been shown that unprotected nucleosides in the syn conformation have Δ(C-2'-C-3') of less than 0.5 ppm, and those preferentially in the anti conformation exhibit Δ(C-2'-C-3') greater than 2.8 ppm.
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85038553865
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Ado = 16.80 min).
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30
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0014229812
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25. We determined the pKa of 1 and Ado in aqueous solution using a micro titrimetric procedure (1, pKa = 2.95 vs 3.59 for Ado; Lit. pKa value for Ado: 3.52; see Clauwaert, J.; Stockx, J.; Z. Naturforsch 1968, 23b, 25).
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