Basic fibroblast growth factor enhances the growth and expression of the osteogenic phenotype of dexamethasone-treated human bone marrow-derived bone-like cells in culture

Bone

Volumn 23, Issue 2, 1998, Pages 111-117

  Pri Chen, S ^a   Pitaru, S ^a   Lokiec, F ^a   Savion, Naphtali ^a,b  

^a TEL AVIV UNIVERSITY   (Israel)

^b SHEBA MEDICAL CENTER   (Israel)

Author keywords

Basic fibroblast growth factor; Bone marrow; Dexamethasone; Human bone cell; Mineralization; Osteoblast

Indexed keywords

ALKALINE PHOSPHATASE; BASIC FIBROBLAST GROWTH FACTOR; CELL DNA; CELL PROTEIN; DEXAMETHASONE; OSTEOCALCIN;

ARTICLE; BONE CELL; BONE DEVELOPMENT; BONE MINERALIZATION; CELL COUNT; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; DNA SYNTHESIS; ELECTRON MICROSCOPY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN SECRETION; SKIN FIBROBLAST;

ADOLESCENT; ALKALINE PHOSPHATASE; ANTHRAQUINONES; BONE AND BONES; BONE MARROW CELLS; CELL COUNT; CELL DIVISION; CELLS, CULTURED; DEXAMETHASONE; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; FIBROBLAST GROWTH FACTOR 2; GLUCOCORTICOIDS; HUMANS; MICROSCOPY, ELECTRON; OSTEOCALCIN; OSTEOGENESIS; PHENOTYPE; PROTEINS; STROMAL CELLS;

EID: 0032146199     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S8756-3282(98)00087-8     Document Type: Article

References (25)

1. 1. Bellows, C. G., Heersche, J. N. M., and Aubin, J. E. Determination of the capacity for proliferation and differentiation of osteoprogenitor cells in the presence and absence of dexamethasone. Dev Biol 140:132-138; 1990.
2. 2. Boudreaux, J. M. and Towler, D. A. Synergistic induction of osteocalcin gene expression. Identification of a bipartite element conferring fibroblast growth factor 2 and cyclic AMP responsiveness in the rat osteocalcin promoter. J Biol Chem 271:7508-7515; 1996.
3. 3. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254; 1976.
4. 4. Cheng, S. L., Yang, J. W., Rifas, L., Zhang, S. F., and Avioli, L. V. Differentiation of human bone marrow osteogenic stromal cells in vitro: Induction of the osteoblast phenotype by dexamethasone. Endocrinology 134: 277-286; 1994.
5. 5. Cheng, S. L., Zhang, S. F., and Avioli, L. V. Expression of bone matrix proteins during dexamethasone-induced mineralization of human bone marrow stromal cells. J Cell Biochem 61:182-193; 1996.
6. 6. Globus, R. K., Patterson-Buckendahl, P., and Gospodarowicz, D. Regulation of bovine bone cell proliferation by fibroblast growth factor and transforming growth factor. Endocrinology 123:98-105; 1988.
7. 7. Globus, R. K., Plouet, J., and Gospodarowicz, D. Cultured bovine bone cells synthesize basic fibroblast growth factor and store it in their extracellular matrix. Endocrinology 124:1539-1547; 1989.
8. 8. Gundle, R. and Beresford, J. N. The isolation and culture of cells from explants of human trabecular bone. Calcif Tissue Int 56(Suppl.):S8-S10; 1995.
9. 9. Hanada, K., Dennis, J. E., and Caplan, A. I. Stimulatory effects of basic fibroblast growth factor and bone morphogenetic protein-2 on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. J Bone Miner Res 12:1606-1614; 1997.
10. 10. Herbertson, A. and Aubin, J. E. Dexamethasone alters the subpopulation make-up of rat bone marrow stromal cell cultures. J Bone Miner Res 10:285-294; 1995.
11. 11. Kamalia, N., McCulloch, C. A., Tenenbaum, H. C., and Limeback, H. Dexamethasone recruitment of self-renewing osteoprogenitor cells in chick bone marrow stromal cell cultures. Blood 79:320-326; 1992.
12. 12. Karnovsky, M. J. A formaldehyde-glutaraldhyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137-138; 1965.
13. 13. Kasperk, C., Schneider, U., Sommer, U., Niethard, F., and Ziegler, R. Differential effects of glucocorticoids on human osteoblastic cell metabolism in vitro. Calcif Tissue Int 57:120-126; 1995.
14. 14. Koshihara, Y., Kawamura, M., Endo, S., Tsutsumi, C., Kodama, H., Oda, H., et al. Establishment of human osteoblastic cells derived from periosteum in culture. In Vitro Cell Dev Biol. 25:37-43; 1989.
15. 15. Long, M. W., Robinson, J. A., Ashcraft, E. A., and Mann, K. G. Regulation of human bone marrow-derived osteoprogenitor cells by osteogenic growth factors. J Clin Invest 95:881-887; 1995.
16. 16. Maniatopoulos, C., Sodek, J., and Melcher, A. H. Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res 254:317-330; 1988.
17. 17. Martin, I., Muraglia, A., Campanile, G., Cancedda, R., and Quarto, R. Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Endocrinology 138:4456-4462; 1997.
18. 18. Noff, D., Pitaru, S., and Savion, N. Basic fibroblast growth factor enhances the capacity of bone marrow cells to form bone-like nodules in vitro. FEBS Lett 250:619-621; 1989.
19. 19. Owen, M. Marrow stromal stem cells. J Cell Sci 10(Suppl.):63-76; 1988.
20. 20. Pitaru, S., Kotev-Emeth, S., Noff, D., Kaffuler, S., and Savion, N. Effect of basic fibroblast growth factor on the growth and differentiation of adult stromal bone marrow cells: Enhanced development of mineralized bone-like tissue in culture. J Bone Miner Res 8:919-929; 1993.
21. 21. Rodan, S. B., Wesolowski, G., Yoon, K., and Rodan, G. A. Opposing effects of fibroblast growth factor and pertussis toxin on alkaline phosphatase, osteopontin, osteocalcin, and type I collagen mRNA levels in ROS 17/2.8 cells. J Biol Chem 264:19934-19941; 1989.
22. 22. Rodan, S. B., Wesolowski, G., Yoon, K., and Rodan, G. A. Opposing effects of fibroblast growth factor and pertussis toxin on alkaline phosphatase, osteopontin, osteocalcin, and type I collagen mRNA levels in ROS 17/2.8 cells. J Biol Chem 264:19934-19941; 1989.
23. 23. Schedlich, L. J., Flanagan, J. L., Crofts, L. A., Gillies, S. A., Goldberg, D., Morrison, N. A., et al. Transcriptional activation of the human osteocalcin gene by basic fibroblast growth factor. J Bone Miner Res 9:143-152; 1994.
24. 24. Subramaniam, M., Colvard, D., Keeting, P. E., Rasmussen, K., Riggs, B. L., and Spelsberg, T. C. Glucocorticoid regulation of alkaline phosphatase, osteocalcin, and proto-oncogenes in normal human osteoblast-like cells. J Cell Biochem 50:411-424; 1992.
25. 25. Wergedal, J. E., Mohan, S., Lundy, M., and Baylink, D. J. Skeletal growth factor and other growth factors known to be present in bone matrix stimulate proliferation and protein synthesis in human bone cells. J Bone Miner Res 5:179-186; 1990.