Purification and characterization of recombinant Thermotoga maritima dihydrofolate reductase

European Journal of Biochemistry

Volumn 255, Issue 3, 1998, Pages 628-637

  Wilquet, Valérie ^a   Gaspar, Joe A ^d   Van De Lande, Monica ^d   Van De Casteele, Mark ^b,c   Legrain, Christianne ^a   Meiering, Elizabeth M ^d   Glansdorff, Nicolas ^a,b,c,e  

^a Institut de Recherches du CERIA   (Belgium)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^c DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^d UNIVERSITY OF WATERLOO   (Canada)

^e Onderzoekingsinstituut CERIA COOVI ^*  (Belgium)

Author keywords

Dihydrofolate reductase; Expression in Escherichia coli; Purification; Thermal stability; Thermotoga maritima

Indexed keywords

DIHYDROFOLATE REDUCTASE; RECOMBINANT PROTEIN;

ANAEROBIC BACTERIUM; ARTICLE; CIRCULAR DICHROISM; DIMERIZATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN EXPRESSION; THERMOSTABILITY;

AMINO ACID SEQUENCE; BACTERIA, ANAEROBIC; CIRCULAR DICHROISM; CLONING, MOLECULAR; FOLIC ACID ANTAGONISTS; HYDROGEN-ION CONCENTRATION; LIGHT; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; SCATTERING, RADIATION; TETRAHYDROFOLATE DEHYDROGENASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA; THERMOTOGA MARITIMA;

EID: 0032145626     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2550628.x     Document Type: Article

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