Separation of natural populations of coliform bacteria from freshwater and sewage by magnetic-bead cell sorting

Journal of Microbiological Methods

Volumn 33, Issue 3, 1998, Pages 221-226

  Porter, Jonathan ^a,b   Pickup, Roger W ^b  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

Author keywords

Coliform bacteria; Concanavalin A; Magnetic beads; Separation

Indexed keywords

FRESH WATER; LECTIN;

ARTICLE; BACTERIAL GROWTH; COLIFORM BACTERIUM; ESCHERICHIA COLI; MAGNETISM; NONHUMAN; PRIORITY JOURNAL; RIVER; SAMPLING; SEWAGE; TECHNIQUE;

ESCHERICHIA COLI;

EID: 0032142982     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(98)00057-8     Document Type: Article

References (15)

1. Bennett A.R., MacPhee S., Betts R.P. The isolation and detection of Escherichia coli O157 by use of immunomagnetic separation and immunoassay procedures. Lett. Appl. Microbiol. 22:1996;237-243.
2. Fry, J.C., 1993. One-way analysis of variance. In: Fry, J.C. (Ed.), Biological Data Analysis. A Practical Approach. IRL Press, Oxford, pp. 1-39.
3. Mansfield L.P., Forsythe S.J. Immunomagnetic separation as an alternative to enrichment broths for Salmonella detection. Lett. Appl. Microbiol. 16:1993;122-125.
4. Payne M.J., Campbell S., Patchett R.A., Kroll R.G. The use of immobilised lectins in the separation of Staphylococcus aureus, Escherichia coli, Listeria and Salmonella spp. from pure cultures and foods. J. Appl. Bacteriol. 73:1992;41-52.
5. Payne M.J., Campbell S., Kroll R.G. Separation of bacteria using agglutinins isolated from invertebrates. J. Appl. Bacteriol. 74:1993;276-283.
6. Porter J., Edwards C., Morgan J.A.W., Pickup R.W. Rapid, automated separation of specific bacteria from lakewater and sewage using flow cytometry and cell sorting. Appl. Environ. Microbiol. 59:1993;3327-3333.
7. Porter J., Robinson J., Pickup R., Edwards C. Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting. FEMS Microbiol. Lett. 133:1995;195-199.
8. Porter, J., Mobbs, K., Hart, C.A., et al., 1997a. Detection, distribution and probable fate of Escherichia coli O157 from asymptomatic cattle on a dairy farm. J. Appl. Microbiol. 83, 297-306.
9. Porter, J., Pickup, R.W., Edwards, C., 1997b. Evaluation of flow cytometric methods for the detection and viability assessment of bacteria in soil. Soil Biol. Biochem. 29, 91-100.
10. Porter J., Robinson J., Pickup R., Edwards C. An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria. J. Appl. Microbiol. 84:1998;722-732.
11. Porter, J., Pickup, R., 1998. Magnetic particle based separation techniques for monitoring bacteria from natural environments. In: Edwards, C. (Ed.), Environmental Monitoring of Bacteria. Humana Press, Totowa, NJ, in press.
12. Reasoner D.J., Geldreich E.E. A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol. 49:1985;1-7.
13. Safarik I., Safarikova M., Forsythe S.J. The application of magnetic separations in applied microbiology. J. Appl. Microbiol. 78:1995;575-585.
14. Skjerve E., Rorvik L.M., Olsvik O. Detection of Listeria monocytogenes in foods by immunomagnetic separation. Appl. Environ. Microbiol. 56:1990;3478-3481.
15. Wallner G., Fuchs B., Spring S., Beisker W., Amann R. Flow sorting of microorganisms for molecular analysis. Appl. Environ. Microbiol. 63:1997;4223-4231.