Purification and characterization of recombinant human granulocyte colony-stimulating factor (rhG-CSF) derivatives: KW-2228 and other derivatives

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 8, 1998, Pages 1528-1534

  Yamasaki, Motoo ^a   Konishi, Noboru ^a   Itoh, Seiga ^a   Yokoo, Yoshiharu ^a  

^a KYOWA HAKKO KOGYO CO LTD   (Japan)

Author keywords

Human granulocyte colony stimulating factor; Physicochemical properties; Protein engineering

Indexed keywords

AMINO ACID; ANTINEOPLASTIC AGENT; GRANULOCYTE COLONY STIMULATING FACTOR; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOASSAY; CHEMISTRY; CIRCULAR DICHROISM; COLORIMETRY; CRYSTALLIZATION; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR WEIGHT; MOUSE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; PROTEIN FOLDING; SEQUENCE ANALYSIS;

AMINO ACIDS; ANIMALS; ANTINEOPLASTIC AGENTS; BIOLOGICAL ASSAY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; COLORIMETRY; CRYSTALLIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GRANULOCYTE COLONY-STIMULATING FACTOR; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MOLECULAR WEIGHT; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS;

EID: 0032134343     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.1528     Document Type: Article

References (26)

1. Metcalf, D., The granulocyte-macrophage colony-stimulating factors. Science, 229, 16-22 (1985).
2. Metcalf, D., Hemopoietic regulators. TIBS, 17, 286-289 (1992).
3. Clark, S. C. and Kamen, R., The human hematopoietic colony-stimulating factors. Science, 236, 1229-1237 (1987).
4. Cowling, G. J. and Dexter, T. M., Erythropoietin and myeloid colony stimulating factors. Trends Biotech., 10, 349-357 (1992).
5. Tamura, M., Ueyama, Y., Nagata, S., Shirafuji, N., and Asano, S., Induction of neutrophilic granulocytosis in mice by administration of purified human native granulocyte colony-stimulating factor (G-CSF) Biochem. Biophys. Res. Commun., 142, 454-460 (1987).
6. Welte, K., Bonilla, M. A., Gillio, A. P., Boone, T. C., Potter, G. K. Gabrilove, J. L., Moore, M. A. S., O’reilly, R. J., and Souza, L. M., Recombinant human granulocyte colony-stimulating factor. Effects on hematopoiesis in normal and cyclophosphamide-treated primates. J. Exp. Med., 165, 941-948 (1987).
7. Kobayashi, Y., Okabe, T., Urabe, A., Suzuki, N., and Takaku, F., Human granulocyte colony-stimulating factor produced by Escherichia coli shortens the period of granulocytopenia induced by irradiation in mice. Jpn. J. Cancer Res., 78, 763-766 (1987).
8. Shimamura, M., Kobayashi, Y., Yuo, A., Urabe, A., Okabe, T., Komatsu, Y., Itoh, S., and Takaku, F., Effect of human recombinant granulocyte colony-stimulating factor on hematopoietic injury in mice induced by 5- fluor our acil. Blood, 69, 353-355 (1987).
9. Morstyn, G., Campbell, L., Souza, L. M., Alton, N. K., Keech, J., Green, M., Sheridan, W., Metcalf, D., and Fox, R., Effect of granulocyte colony stimulating factor on neutropenia induced by cytotoxic chemotherapy. Lancet, 667-672 (1988).
10. Teshima, H., Ishikawa, J., Kitayama, H., Yamagami, T., Hiraoka, A., Nakamura, H., Shibata, H., Masaoka, T., and Takaku, F., Clinical effects of recombinant human granulocyte colony-stimulating factor in leukemia patients: a phase I/II study. Exp. Hematol., 17, 853-858 (1989).
11. Nagata, S., Tsuchiya, M., Asano, S., Kaziro, Y., Yamazaki, T., Yamamoto, O., Hitata, Y., Kubota, N., Oheda, M., Nomura, H., and Ono, M., Molecular cloning and expression of cDNA for human granulocyte colony- stimulating factor. Nature, 319, 415-418 (1986).
12. Souza, L. M., Boone, T. C., Gabrilove, J., Lai, P. H., Zsebo, K. M., Murdock, D., Chazin, V. R., Bruszewski, J., Lu, H., Chen, K. K., Barendt, J., Platzer, E., Moore, M. A. S., Mertelsmann, R., and Welte, K., Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells. Science, 232, 61-65 (1986).
13. Komatsu, Y., Matsumoto, T., Kuga, T., Nishi, T., Sekine, S., Saito, A., Okabe, M, Morimoto, M., Itoh, S., Okabe, T., and Takaku, F., Cloning of granulocyte colony-stimulating factor cDNA and its expression in Escherichia coli. Jpn. J. Cancer Res., 78, 1179-1181 (1987).
14. Kuga, T., Komatsu, Y., Yamasaki, M., Sekine, S., Miyaji, H., Nishi, T., Sato, M., Yokoo, Y., Asano, M., Okabe, M., Morimoto, M., and Itoh, S., Mutagenesis of human granulocyte colony stimulating factor. Biochem. Biophys. Res. Commun., 159, 103-111 (1989).
15. Okabe, M., Asano, M., Kuga, T., Komatsu, Y., Yamasaki, M., Yokoo, Y., Itoh, S., Morimoto, M., and Oka, T., In vitro and in vivo hematopoietic effect of mutant granulocyte colony-stimulating factor. Blood, 75, 1788-1793 (1990).
16. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J., Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951).
17. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
18. Heinrikson, R. L., and Meredith, S.C., Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate. Anal. Biochem., 136, 65-74 (1984).
19. Holmes, K. L., Palaszynski, E., Fredrickson, T. N., Morse III, H. C., and Ihle, J. N., Correlation of cell-surface phenotype with the establishment of interleukin 3-dependent cell lines from wild-mouse murine leukemia virus-induced neoplasms. Proc. Natl. Acad. Sci. USA, 82, 6687-6691 (1985).
20. Asano, M., Gomi, K., and Okabe, M., A colorimetric microassay for the biological activity of human granulocyte colony-stimulating factor using murine myeloblastic leukemia, NFS-60 cells. Jpn. Pharmacol. Ther., 19, 2161-2113 (1991).
21. Lu, H. S., Boone, T. C., Souza, L. M., and Lai, P.-H., Dis-ulfide and secondary structures of recombinant granulocyte colony stimulating factor. Arch. Biochem. Biophys., 268, 81-92 (1989).
22. Yamamoto, A., Toda, H., and Sakiyama, F., Vapor-phase hydrazinolysis for microdetermination of carboxyl-terminal amino acid of proteins. J. Biochem., 106, 552-554 (1989).
23. Greenfield, N., and Fasman, G. D., Computed circular dichro-ism spectra for the evaluation of protein conformation. Biochemistry, 8, 4108-4116 (1969).
24. Perry, L. J., and Wetzel, R., Disulfide bond engineered in to T4 lyoszyme: Stabilization of the protein toward thermal inactivation. Science, 226, 555-557 (1984).
25. Hill, C. P., Osslund, T. M., and Eisenberg, D., The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors. Proc. Natl. Acad. Sci. USA, 90, 5167-5171 (1993).
26. Fujii, I., Nagahara, Y., Yamasaki, M., Yokoo, Y., Itoh, S., and Hirayama, N., Structure of KW-2228, a tailored human granulocyte colony-stimulating factor with enhanced biological activity and stability. FEBS Lett., 410, 131-135 (1997).