Mechanisms of acute desensitization of the β2AR-adenylyl cyclase pathway in human airway smooth muscle

American Journal of Respiratory Cell and Molecular Biology

Volumn 19, Issue 2, 1998, Pages 338-348

  Penn, Raymond B ^a   Panettieri Jr , Reynold A ^b   Benovic, Jeffrey L ^b  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ADENYLATE CYCLASE; BETA 2 ADRENERGIC RECEPTOR; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; CYCLIC AMP DEPENDENT PROTEIN KINASE; PROTEIN KINASE C;

ARTICLE; CELL CULTURE; CYTOLOGY; DRUG POTENTIATION; ENZYMOLOGY; HUMAN; METABOLISM; SIGNAL TRANSDUCTION; SMOOTH MUSCLE; TRACHEA;

ADENYLATE CYCLASE; ADRENERGIC BETA-AGONISTS; CELLS, CULTURED; CYCLIC AMP-DEPENDENT PROTEIN KINASES; HUMANS; MUSCLE, SMOOTH; PROTEIN KINASE C; RECEPTORS, ADRENERGIC, BETA-2; SIGNAL TRANSDUCTION; TRACHEA;

EID: 0032133342     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.19.2.3025     Document Type: Article

References (55)

1. Penn, R. B., and J. L. Benovic. 1998 Desensitization of G protein-coupled receptors. In Handbook of Physiology, Vol. 1. P. M. Conn, editor. Oxford University Press, New York. 125-164.
2. Jack, D. 1991. A way of looking at agonism and antagonism: lessons from salbutamol, salmeterol, and other beta-adrenoceptor agonists. Br. J. Clin. Pharmacol. 31:501-514.
3. Johnson, M. 1995. Pharmacology of long-acting β-agonists. Ann. Allergy Asthma Immunol. 75:177-179.
4. Cockcroft, D. W., C. P. McParland, S. A. Britto, V. A. Swystun, and B. C. Rutherford. 1993. Regular inhaled salbutamol and airway responsiveness to allergen. Lancet 342:833-837.
5. Sears, M. R., D. R. Taylor, C. G. Print, D. C. Lake, Q. Li, E. M. Flannery, D. M. Yates, M. K. Lucas, and G. P. Herbison. 1990. Regular inhaled beta-agonist treatment in bronchial asthma. Lancet 336:1391-1396.
6. Spitzer, W. O., S. Suissa, P. Ernst, R. I. Horwitz, B. Habbick, D. Cockroft, J.-F. Boivin, M. McNutt, A. S. Buist, and A. S. Rebuck. 1992. The use of β-agonists and the risk of death and near-death from asthma. N. Engl. J. Med. 326:501-506.
7. Vathenen, A. S., B. G. Higgins, A. J. Knox, J. R. Britton, and A. E. Tattersfield. 1988. Rebound increase in bronchial responsiveness after treatment with inhaled terbutaline. Lancet 1:554-558.
8. Shih, M., and C. C. Malbon. 1994. Oligodeoxynucleotides antisense to mRNA encoding protein kinase A, protein kinase C, and β-adrenergic receptor kinase reveal distinctive cell-type specific roles in agonist-induced desensitization. Proc. Natl. Acad. Sci. USA 91:12193-12197.
9. 2-adrenergic receptor. J. Biol. Chem. 272:7338-7344.
10. Panettieri, R. A., R. K. Murray, L. R. DePalo, P. A. Yadvish, and M. I. Kotlikoff. 1989. A human smooth muscle cell line that retains physiological responsiveness. Am. J. Physiol. 256 (Cell. Physiol. 25):C329-C335.
11. Panettieri, R. A. 1994. Airway smooth muscle cell growth and proliferation. In Airway Smooth Muscle: Development, Regulation, and Contractility. D. Racburn and M. A. Giembycz, editors. Birkhauser Verlag, Basel.
12. 2+ imaging and perforated patch-clamp techniques. Am. J. Physiol. 264 (Cell. Physiol. 33):C485-C490.
13. 2-adrenoceptor desensitization in cultured human airway smooth muscle. Clin. Sci. 84:151-157.
14. Bradford, M. 1976. A rapid and sensitive method for the quantification of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
15. Scatchard, G. 1949. The attraction of proteins for small molecules and ions. Ann. N.Y. Acad. Sci. 51:660-672.
16. 2-mediated adenylyl cyclasc activity in human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 11:496-505.
17. Ferguson, S. S. G., W. E. I. Downey, A.-M. Colapietro, L. S. Barak, L. Menard, and M. G. Caron. 1996. Role of β-arrestin in mediating agonist-promoted G protein-coupled receptor internalization. Science 271:363-365.
18. 2-adrenergic receptor. J. Biol. Chem. 269:13084-13087.
19. Pereira, M. E., D. L. Segaloff, M. Ascoli, and F. Eckstein. 1987. Inhibition of choriogonadotropin-activated steroidogenesis in cultured Leydig tumor cells by the Rp diastereoisomer of adenosine 3′,5′-cyclic phosphorothiate. J. Biol. Chem. 262:6093-6100.
20. Forsayeth, J. R., and P. B. Garcia. 1994. Adenovirus-mediated transfection of cultured cells. Biotechniquesl 17:354-359.
21. Lim, K., and C. B. Chae. 1989. A simple assay for DNA transfection by incubation of the cells in culture dishes with substrates for heta-galactosidase. Biotechniques 7:576-579.
22. Benovic, J. L., A. De Blasi, W. C. Stone, M. G. Caron, and R. J. Lefkowitz. 1989. β-adrenergic receptor kinase: primary structure delineates a multigene family. Science 246:235-240.
23. Loudon, B., B. Perussia, and J. L. Benovic. 1996. Differentially regulated expression of the G protein-coupled receptor kinases. βARK and GRK6, during myelomonocytic cell development in vitro. Blood 88:4547-4557.
24. 2-adrenergic receptor desensitization. Delineation with specific inhibitors. J. Biol. Chem. 265:3202-3209.
25. 2-adrenergic receptor genotypes on signal transduction in human airway smooth muscle cells. Am. J. Respir. Cell Mol. Biol. 13:25-33.
26. Hall, I. P., and M. Kotlikoff. 1995. Use of cultured airway myocytes for study of airway smooth muscle. Am. J. Physiol. 268:L1-L11.
27. 2-adrenergic receptor signaling in human airways. Am. J. Physiol. 271 (Lung Cell Mol. Physiol. 15):L601-L608.
28. Homburger, V., M. Lucas, B. Cantau, J. Barabe, J. Penit, and J. Bockaert. 1980. Further evidence that desensitization of beta-adrenergic-sensitive adenylate cyclase proceeds in two steps: modification of the coupling and loss of beta-adrenergic receptors. J. Biol. Chem. 255:10436-10444.
29. Waldo, G. L., J. K. Northup, J. P. Perkins, and T. K. Harden. 1983. Characterization of an altered membrane form of the β-adrenergic receptor produced during agonist-induced desensitization. J. Biol. Chem. 258:13900-13908.
30. Yu, S. S., and R. J. Lefkowitz. 1993. β-adrenergic receptor sequestration: a potential mechanism of receptor resensitization. J. Biol. Chem. 268:337-341.
31. Pippig, S., S. Andexinger, and M. J. Lohse. 1995. Sequestration and recycling of beta 2-adrenergic receptors permit receptor resensitization. Mol. Pharmacol. 47:666-676.
32. 2-adrenergic receptor. J. Biol. Chem. 268:3201-3208.
33. 1-adrenergic receptor. J. Biol. Chem. 270:17953-17961.
34. 2-adrenoceptors of mononuclear blood cells in asthmalic children. Eur. J. Pediatr. 150:209-213.
35. Hayes, M., F. Qing. C. G. Rhodes, P. W. Ind, T. Jones, and J. M. B. Hughes. 1994. Effect of two weeks of salbutamol on human pulmonary beta-adrenergic receptor number and airway function. Eur. Respir. J. 7:235s.
36. 2-adrenergic receptors desensitize in response to in vivo administered β-agonist. Am. J. Physiol. 269 (Lung Cell. Mol. Physiol. 5):L709-L714.
37. Davis, C., and M. E. Conolly. 1980. Tachyphylaxis to beta-adrenoceptor agonists in human bronchial smooth muscle: studies in vitro. Br. J. Clin. Pharmacol. 10:417-423.
38. 1 modulates β-adrenergic receptor number and function in cultured human tracheal smooth muscle cells. Am. J. Physiol. 266 (Lung Cell. Mol. Physiol. 10):L187-L191.
39. Nogami, M., S. M. Whittle, D. J. Romberger, S. I. Rennard, and M. Toews. 1995. Lysophosphatidic acid regulation of cyclic AMP accumulation in cultured human airway smooth muscle cells. Mol. Pharmacol. 48:766-773.
40. Sterne-Marr, R., and J. L. Benovic. 1995. Regulation of G protein-coupled receptors by receptor kinases and arrestins. In Vitamins and Hormones. G. Litwack, editor. Academic Press, New York. 193-234.
41. Pitcher, J. A., J. Inglese, J. B. Higgins, J. L. Arriza, P. J. Casey, C. Kim, J. L. Benovic, M. M. Kwatra, M. G. Caron, and R. J. Lefkowitz. 1992. Role of Bγ subunits of G proteins in targeting the β-adrenergic recepror kinase to membrane-bound receptors. Science 257:1264-1267.
42. Lohse, M. J., J. L. Benovic, M. G. Caron, and R. J. Lefkowitz. 1990. β-Arrestin: a protein that regulates β-adrenergic receptor function. Science 248:1547-1550.
43. 2-adrenergic, and m2 muscarinic cholinergic receptors. J. Biol. Chem. 270:720-731.
44. Goodman, O. B., J. G. Krupnick, F. Santini, V. V. Gurevich, R. B. Penn, A. W. Gagnon, J. H. Keen, and J. L. Benovic. 1996. Beta-arrestin acts as a clathrin adaptor protein in endocytosis of the beta-2-adrenergic receptor. Nature 383:447-450.
45. Strasser, R. H., D. R. Sibley and R. J. Lefkowitz. 1986. A novel catecholamine-activated adenosine cyclic 3′,5′-phosphate independent pathway for b-adrenergic receptor phosphorylation in wild-type and mutant s49 lymphoma cells: mechanism of homologous desensitization of adenylate cyclase. Biochemistry 25:1371-1377.
46. Green, D. A., J. Friedman, and R. B. Clark. 1981. Epinephrine desensitization of adenylate cyclase from cyc-and s49 cultured lymphoma cells. J. Cyclic Nucleotide Res. 7:161-172.
47. Green, D. A., and R. B. Clark. 1981. Adenylate cyclase coupling proteins are not essential for agonist-specific desensitization of lymphoma cells. J. Biol. Chem. 256:2105-2108
48. Shear, M., P. A. Insel, K. I., Melmon, and P. Coffino. 1976. Agonist-specific refractoriness induced by isoproterenol: a sludy with mutani cells. J. Biol. Chem. 251:7572-7576.
49. Insel, P. A., L. C. Mahan, H. J. Motulsky, L. M. Stoolman, and A. M. Koachman. 1983. Time-dependent decreases in hinding affinity of agonists for β-adrenergic receptors of intact s49 lymphoma cells: a mechanism of desensitization. J. Biol. Chem. 258:13597-13605.
50. Roth, N. S., P. T. Campbell, M. G. Caron, R. J. Lefkowitz, and M. J. Lohse. 1991. Comparative rates of desensitization of β-adrenergic receptors by the β-adrenergic receptor kinase and the cyclic AMP-dependent protein kinase. Proc. Natl. Acad Sci. USA 88:6201-6204.
51. 2-adrenergic receptors lacking phosphorylation sites. Mol. Pharmacol. 36:641-646.
52. 2-adrenergic receptor are involved in distinct pathways of receptor desensitization. J. Biol. Chem. 264:12657-12665.
53. 2-adrenergic receptor pathway in s49 lymphoma cells. J. Biol. Chem. 271:895-900.
54. Choi, D, J., W. J. Koch, J. J. Hunter, and H. A. Rockman. 1997. Mechanism of beta-adrenergic receptor desensitization in cardiac hypertrophy is increased beta-adrenergic receptor kinase. J. Biol. Chem. 272:17223-17229.
55. Ungerer, M., M. Bohm, J. S. Elce, E. Erdmann, and M. J. Lohse. 1993. Altered expression of the β-adrenergic receptor kinase and β1-adrenergic receptors in failing human heart. Circulation 87:454-463.