Molecular mechanism of polyhomeotic activation by Engrailed

EMBO Journal

Volumn 17, Issue 13, 1998, Pages 3704-3713

  Serrano, Nuria ^a,b   Maschat, Florence ^a  

^a INSTITUT JACQUES MONOD   (France)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Drosophila; Engrailed; Extradenticle; polyhomeotic; Transcriptional activation

Indexed keywords

HOMEODOMAIN PROTEIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DNA FOOTPRINTING; DROSOPHILA; EMBRYO DEVELOPMENT; GENE ACTIVATION; GENE LOCUS; GENE OVEREXPRESSION; GENE REPRESSION; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION INITIATION;

ANIMALS; BASE SEQUENCE; BINDING SITES; CELL LINE; CHROMOSOME MAPPING; DNA-BINDING PROTEINS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; HOMEODOMAIN PROTEINS; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; NUCLEOPROTEINS; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS;

EID: 0032127558     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.13.3704     Document Type: Article

References (37)

1. Bourbon, H.M., Martin-Blanco, E., Rosen, D. and Kornberg, T.B. (1995) Phosphorylation of the Drosophila Engrailed protein at a site outside its homeodomain enhances DNA binding. J. Biol. Chem., 270, 11130-11139.
2. Chan, S.K., Jaffe, L., Capovilla, M., Botas, J. and Mann, R.S. (1994) The DNA binding specificity of Ultrabithorax is modulated by cooperative interactions with Extradenticle, another homeoprotein. Cell, 78, 603-615.
3. Deatrick, J., Daly, M., Randsholt, N.B. and Brock, H.W. (1991) The complex genetic locus polyhomeotic in Drosophila melanogaster potentially encodes two homologous zinc-finger proteins. Gene, 105, 185-195.
4. Desplan, C., Theis, J. and O'Farrell, P.H. (1985) The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity. Nature, 318, 630-635.
5. Desplan, C., Theis, J. and O'Farrell, P.H. (1988) The sequence specificity of homeodomain-DNA interaction. Cell, 54, 1081-1090.
6. Dobens, L., Rudolph, K. and Berger, E.M. (1991) Ecdysterone regulatory elements function as both transcriptional activators and repressors. Mol. Cell. Biol., 11, 1846-1853.
7. Dura, J.M., Randsholt, N.B., Deatrick, J., Erk, I., Santamaria, P., Freeman, J.D., Freeman, S.J., Weddell, D. and Brock, H.W. (1987) A complex genetic locus, polyhomeotic, is required for segmental specification and epidermal development in D.melanogaster. Cell, 51, 829-839.
8. Eaton, S. and Kornberg, T.B. (1990) Repression of ci-D in posterior compartments of Drosophila by engrailed. Genes Dev., 4, 1068-1077.
9. Fauvarque, M.O., Zuber, V. and Dura, J.M. (1995) Regulation of polyhomeotic transcription may involve local changes in chromatin activity in Drosophila. Mech. Dev., 52, 343-345.
10. Fjose, A., McGinnis, W.J. and Gehring, W.J. (1985) Isolation of a homeo box-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts. Nature, 313, 284-289.
11. Gay, N.J., Poole, S.J. and Kornberg, T.B. (1988) The Drosophila engrailed protein is phosphorylated by a serine-specific protein kinase. Nucleic Acids Res., 16, 6637-6647.
12. Gorman, C.M., Moffat, L.F. and Howard, B.H. (1982) Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol., 2, 1044-1051.
13. Han, K. and Manley, J.L. (1993) Functional domains of the Drosophila Engrailed protein. EMBO J., 12, 2723-2733.
14. Han, K., Levine, M. and Manley, J. (1989) Synergistic activation and repression of transcription by Drosophila homeobox proteins. Cell, 56, 573-583.
15. Heemskerk, J., DiNardo, S., Kostriken, R. and O'Farrell, P.H. (1991) Multiple modes of engrailed regulation in the progression towards cell fate determination. Nature, 352, 404-410.
16. Ingham, P.W. (1988) The molecular genetics of embryonic pattern formation in Drosophila. Nature, 335, 25-34.
17. Jaynes, J.B. and O'Farrell, P.H. (1988) Activation and repression of transcription by homeodomain-containing proteins that bind a common site. Nature, 336, 744-749.
18. Jaynes, J.B. and O'Farrell, P.H. (1991) Active repression of transcription by the Engrailed homeodomain protein. EMBO J., 10, 1427-1433.
19. John, A., Smith, S.T. and Jaynes, J.B. (1995) Inserting the Ftz homeodomain into Engrailed creates a dominant transcriptional repressor that specifically turns off Ftz target genes in vivo. Development, 121, 1801-1813.
20. Kissinger, C.R., Liu, B., Martin-Blanco, E., Kornberg, T.B. and Pabo, C.O. (1990) Crystal structure of an Engrailed homeodomain-DNA complex at 2.8 Å resolution: a framework for understanding homeodomain-DNA interactions. Cell, 63, 579-590.
21. Kornberg, T. (1981a) Compartments in the abdomen of Drosophila and the role of the engrailed locus. Dev. Biol., 86, 363-372.
22. Kornberg, T. (1981b) engrailed: a gene controlling compartment and segment formation in Drosophila. Proc. Natl Acad. Sci. USA, 78, 1095-1099.
23. Krasnow, M.A., Saffman, E.E., Kornfeld, K. and Hogness, D.S. (1989) Transcriptional activation and repression by Ultrabithorax proteins in cultured Drosophila cells. Cell, 57, 1031-1043.
24. Lawrence, P.A. and Morata, G. (1976) Compartments in the wing of Drosophila, a study of the engrailed gene. Dev. Biol., 50, 321-337.
25. Mann, R.S. and Abu-Shaar, M. (1996) Nuclear import of the homeodomain protein Extradenticle in response to Wg and Dpp signalling. Nature, 383, 630-633.
26. Maxam, A.M. and Gilbert, W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol., 65, 499-560.
27. Ohkuma, Y., Horikoshi, M., Roeder, R.G. and Desplan, C. (1990) Engrailed, a homeodomain protein, can repress in vitro transcription by competition with the TATA box-binding protein transcription factor IID. Proc. Natl Acad. Sci. USA, 87, 2289-2293.
28. Peifer, M. and Wieschaus, E. (1990) Mutations in the Drosophila gene extradenticle affect the way specific homeo domain proteins regulate segmental identity. Genes Dev., 4, 1209-1223.
29. Poole, S.J., Kauvar, L.M., Drees, B. and Kornberg, T. (1985) The engrailed locus of Drosophila: structural analysis of an embryonic transcript. Cell, 40, 37-43.
30. Rauskolb, C. and Wieschaus, E. (1994) Coordinate regulation of downstream genes by extradenticle and the homeotic selector proteins. EMBO J., 13, 3561-3569.
31. Saenz-Robles, M.T., Maschat, F., Tabata, T. and Kornberg, T.B. (1995) Selection and characterization of sequences with high affinity for the Engrailed protein of Drosophila. Mech. Dev., 53, 185-195.
32. Serrano, N., Brock, H.W., Demeret, C. Dura, J.M., Randsholt, N.B., Kornberg, T.B. and Maschat, F. (1995) polyhomeotic appears to be a target of Engrailed regulation in Drosophila. Development, 121, 1691-1703.
33. Serrano, N., Brock, H.W. and Maschat, F. (1997) β3-Tubulin is directly repressed by the Engrailed protein in Drosophila. Development, 124, 2527-2539.
34. Sullivan, W., Fogarty, P. and Theurkauf, W. (1993) Mutations affecting the cytoskeletal organization of syncitial Drosophila embryos. Development, 118, 1245-1254.
35. Tabata, T., Eaton, S. and Kornberg, T.B. (1992) The Drosophila hedgehog gene is expressed specifically in posterior compartment cells and is a target of engrailed regulation. Genes Dev., 6, 2635-2645.
36. van Dijk, M.A. and Murre, C. (1994) extradenticle raises the DNA binding specificity of homeotic selector gene products. Cell, 78, 617-624.
37. Winslow, G.M., Hayashi, S., Krasnow, M., Hogness, D.S. and Scott, M.P. (1989) Transcriptional activation by the Antennapedia and fushi tarazu proteins in cultured Drosophila cells. Cell, 57, 1017-1030.