Regulation of the transcriptional activators AnfA and VnfA by metals and ammonium in Azotobacter vinelandii

FEMS Microbiology Letters

Volumn 164, Issue 1, 1998, Pages 63-68

  Premakumar, R ^a   Pau, Richard N ^b   Mitchenall, Lesley A ^b   Easo, Mary ^a   Bishop, Paul E ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

AnfA; Azotobacter vinelandii; ModE; Nitrogen fixation; Regulation; VnfA

Indexed keywords

CELL PROTEIN; MOLYBDENUM; NITROGENASE; TRANSCRIPTION ACTIVATOR ANFA; TRANSCRIPTION ACTIVATOR VNFA; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ARTICLE; AZOTOBACTER VINELANDII; ENZYME ACTIVITY; ENZYME MECHANISM; GENE EXPRESSION; GENE FUSION; GENE REPRESSION; NONHUMAN; OPERON; PRIORITY JOURNAL; TRANSCRIPTION REGULATION;

ACETATES; AZOTOBACTER VINELANDII; BACTERIAL PROTEINS; BETA-GALACTOSIDASE; CLONING, MOLECULAR; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; METALS; MOLYBDENUM; TRANS-ACTIVATORS; VANADIUM;

AZOTOBACTER VINELANDII; NEGIBACTERIA;

EID: 0032125494     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00195-5     Document Type: Article

References (21)

1. Bishop, P.E. and Premakumar, R. (1992) Alternative nitrogen fixation systems. In: Biological Nitrogen Fixation (Stacey, G., Burris, R.H. and Evans, H.J., Eds.), pp. 736-762. Chapman and Hall, New York.
2. Jacobitz, S. and Bishop, P.E. (1992) Regulation of nitrogenase 2 in Azotobacter vinelandii by ammonium, molybdenum and vanadium. J. Bacteriol. 174, 3884-3888.
3. Premakumar, R., Chisnell, J.R. and Bishop, P.E. (1989) A comparison of the three dinitrogenase reductases expressed by Azotobacter vinelandii. Can. J. Microbiol. 35, 344-348.
4. Joerger, R.D., Wolfinger, E.D. and Bishop, P.E. (1991) The gene encoding dinitrogenase reductase 2 is required for expression of the second alternative nitrogenase from Azotobacter vinelandii. J. Bacteriol. 173, 4440-4446.
5. Joerger, R.D., Jacobson, M.R. and Bishop, P.E. (1989) Two nifA-like genes required for expression of alternative nitrogenases by Azotobacter vinelandii. J. Bacteriol. 171, 3258-3267.
6. Luque, F. and Pau, R.N. (1991) Transcriptional regulation by metals of structural genes for A. vinelandii nitrogenases. Mol. Gen. Genet. 227, 481-487.
7. Walmsley, J., Toukdarian, A. and Kennedy, C. (1994) The role of regulatory genes nifA, vnfA, anfA, nfrX, ntrC and rpoN in expression of genes encoding the three nitrogenases of Azotobacter vinelandii. Arch. Microbiol. 162, 422-429.
8. Drummond, M., Walmsley, J. and Kennedy, C. (1996) Expression from the nifB promoter of Azotobacter vinelandii can be activated by NifA, VnfA, or AnfA transcriptional activators. J. Bacteriol. 178, 788-792.
9. Drummond, M., Whitty, P. and Wootton, J. (1986) Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins. EMBO J. 5, 441-447.
10. Woodley, P., Buck, M. and Kennedy, C. (1996) Identification of sequences important for recognition of vnf genes by the VnfA transcriptional activator in Azotobacter vinelandii. FEMS Microbiol. Lett. 135, 213-221.
11. Premakumar, R., Loveless, T.M. and Bishop, P.E. (1994) Effect of amino acid substitutions in a potential metal-binding site of AnfA on expression from the anfH promoter in Azotobacter vinelandii. J. Bacteriol. 176, 6139-6142.
12. Frise, E., Green, A. and Drummond, M. (1994) Chimeric transcriptional activators generated in vivo from VnfA and AnfA of Azotobacter vinelandii: N-terminal domain of AnfA is responsible for dependence on nitrogenase Fe-protein. J. Bacteriol. 176, 6545-6549.
13. Austin, S. and Lambert, J. (1994) Purification and in vitro activity of a truncated form of AnfA. Transcriptional activator protein of alternative nitrogenase from Azotobacter vinelandii. J. Biol. Chem. 269, 18141-18148.
14. Anderson, L.A., Palmer, T., Price, N.C., Bornemann, S., Boxer, D.H. and Pau, R.N. (1997) Characterisation of the molybdenum-responsive ModE regulatory protein and its binding to the promoter region of the modABCD (molybdenum transport) operon of Escherichia coli. Eur. J. Biochem. 246, 119-126.
15. McNicholas, P.M., Rech, S.A. and Gunsalus, R.P. (1997) Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli. Mol. Microbiol. 23, 515-524.
16. Mouncey, N.J., Mitchenall, L.A. and Pau, R.N. (1995) Mutational analysis of genes of the mod locus involved in molybdenum transport, homeostasis and processing in Azotobacter vinelandii. J. Bacteriol. 177, 5294-5302.
17. 54 and repressed by molybdenum. J. Bacteriol. 178, 2010-2017.
18. Kokotek, W. and Lotz, W. (1989) Construction of a lacZ-kanamycin resistance cassette, useful for site-directed mutagenesis and as a promoter probe. Gene 84, 467-471.
19. Joerger, R.D., Jacobson, M.R., Premakumar, R., Wolfinger, E.D. and Bishop, P.E. (1989) Nucleotide sequence and mutational analysis of the structural genes for the second alternative nitrogenase from Azotobacter vinelandii. J. Bacteriol. 171, 1075-1086.
20. Grunden, A.M., Ray, R.M., Rosentel, J.K., Healy, F.G. and Shanmugam, K.T. (1996) Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE. J. Bacteriol. 178, 735-744.
21. Bush, J.A. and Wilson, P.W. (1959) A non-gummy chromogenic strain of Azotobacter vinelandii. Nature 184, 381-382.