Characterisation of the molybdenum-responsive ModE regulatory protein and its binding to the promoter region of the modABCD (molybdenum transport) operon of Escherichia coli

European Journal of Biochemistry

Volumn 246, Issue 1, 1997, Pages 119-126

  Anderson, Lisa A ^a,b   Palmer, Tracy ^a   Price, Nicholas C ^c   Bornemann, Stephen ^b   Boxer, David H ^a   Pau, Richard N ^b,c  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b UNIVERSITY OF STIRLING   (United Kingdom)

^c JOHN INNES CENTRE   (United Kingdom)

Author keywords

Binding; Escherichia coli; Molybdenum; Regulation; Transport

Indexed keywords

MOLYBDENUM; REGULATOR PROTEIN;

ARTICLE; CIRCULAR DICHROISM; DENSITY GRADIENT CENTRIFUGATION; DNA BINDING; DNA TRANSCRIPTION; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN MODIFICATION;

EID: 0030917717     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00119.x     Document Type: Article

References (41)

1. Ansari, A. Z., Bradner, J. E. & O'Halloran, T. V. (1995) DNA-bend modulation in a repressor-to activator switching mechanism, Nature 374, 371-375.
2. Bairoch, A. (1992) PROSITE: a dictionary of sites and patterns in proteins, Nucleic Acids Res. 20 (Supplement), 2013-2018.
3. Clark, L. R. & Axley, J. H. (1955) Molybdenum determination in soils and rocks with dithiol, Anal. Chem. 27, 2000-2003.
4. Devereux, J. F., Coulson, A. F. W. & Lyall, A. (1987) Protein and nucleic acid sequence database searching: a suitable case for parallel processing, Nucleic Acids Res. 12, 387-395.
5. Fuchs, R. (1994) Predicting protein function: a versatile tool for the Apple Macintosh, Comput. Appl. Biosci. 10, 171-178.
6. Gallegos, M.-T., Michán, C. & Ramos, J. L. (1993) The XylS/AraC family of regulators, Nucleic Acids Res. 21, 807-810.
7. Gill, S. C. & von Hippel, P. H. (1989) Calculation of protein extinction coefficients from amino acid sequence data, Anal. Biochem. 182, 319-326.
8. Grunden, A. M., Ray, R. M., Rosentel, J. K., Healy, F. G. & Shanmugam, K. T. (1996) Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE, J. Bacteriol. 178, 735-744.
9. Henikoff, S. & Henikoff, J. G. (1994) Protein family classification based on searching a database of blocks, Genomics 19, 97-107.
10. Hinton, S. M. & Freyer, G. (1986) Cloning, expression and sequencing the molybdenum-pterin binding protein (mop) gene of Clostridium pasteurianum in Escherichia coli, Nucleic Acids Res. 14, 9371-9380.
11. Hinton, S. M. & Merritt, B. (1986) Purification and characterisation of a molybdenum-pterin-binding protein (Mop) in Clostridium pasteurianum W5, J. Bacteriol. 168, 688-693.
12. Johann, S. & Hinton, S. (1987) Cloning and nucleotide sequence of the chlD locus, J. Bacteriol. 169, 1911-1916.
13. 54, and repressed by molybdenum, J. Bacteriol. 178, 2010-2017.
14. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
15. Maupin-Furlow, J. A., Rosentel, J. K., Lee, J. H., Deppenmeier, U. & Gunsalus, R. P. (1995) Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli, J. Bacteriol. 177, 4851-4856.
16. Maxam, A. M. & Gilbert, W. (1980) Sequencing end-labelled DNA with base-specific chemical cleavages, Methods Enzymol. 65, 499-500.
17. McNicholas, P. M., Chiang, R. C. & Gunsalus, R. P. (1996) The Escherichia coli modE gene: effects of mutations on molybdate dependent modA expression, FEMS Microbiol. Lett. 145, 117-123.
18. McNicholas, P. M., Rech, S. A. & Gunsalus, R. P. (1997) Characterization of the ModE DNA-binding sites in the control regions of modABCD and modABCDE of Escherichia coli, Mol. Microbiol. 23, 515-524.
19. Miller, J., Scott, D. & Amy, N. (1987) Molybdenum-sensitive transcriptional regulation of the chlD locus of Escherichia coli, J. Bacteriol. 169, 1853-1860.
20. Miller, J. H. (1972) Experiments in molecular genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY.
21. Mouncey, N. J., Mitchenall, L. A. & Pau, R. N. (1995) Mutational analysis of genes of the mod locus involved in molybdenum transport, homeostasis and processing in Azotobacter vinelandii, J. Bacteriol. 177, 5294-5302.
22. Mouncey, N. J., Mitchenall, L. A. & Pau, R. N. (1996) The modE gene product mediates molybdenum-dependant expression of genes for the high-affinity molybate transporter and modG in Azotabacter vinelandii, Microbiology 142, 1997-2004.
23. Murray, V. (1989) Improved double-stranded DNA sequencing using the linear polymerase chain reaction, Nucleic Acids Res, 17, 8889.
24. Pau, R. N., Klipp, W. & Leimkühler, S. (1997) Molybdenum transport, processing and gene regulation, in Iron and related transition metals in microbial metabolism (Winkelmann, G. & Carrano, C. J., eds) pp. 217-234, Harwood Academic Publishers, Amsterdam.
25. Provencher, S. W. & Glöckner, J. (1981) Estimation of globular protein secondary structure from circular dichroism, Biochemistry 20, 33-37.
26. Rajagopalan, K. V. (1993). Biochemistry of the molybdenum cofacors, in Molybdenum enzymes, cofactors and model systems (Stiefel, E. I., Coucouvanis, D. & Newton, W. E., eds) pp. 38-49, American Chemical Society, Washington DC.
27. Rajagopalan, K. V. (1996). Biosynthesis ot the molybdenum cofactor, in Escherichia coli and Salmonella typhimurium (Neidhardt, F. C., ed.) pp. 674-679, American Scociety of Microbiology Press, Washington DC.
28. Rech, S., Deppenmeier, U. & Gunsalus, R. P. (1995) Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availibility, J. Bacteriol. 177, 1023-1029.
29. Rosentel, J. K., Healy, F., Maupin-Furlow, J. A., Lee, J. H. & Shanmugam, K. T. (1995) Molybdenum regulation of mod (Molybdate transport), fdhF, and hyc (formate hydrogen lyase) operons in Escherichia coli, J. Bacteriol. 177, 4857-4864.
30. Rost, B. (1995) TOPITS: Threading one-dimensional predictions into three-dimensional structures, in The third international conference on intelligent systems for molecular biology (ISBM), Cambridge UK July 16-19, 1995, (Rawlings, C., Clark, D., Altman, R., Hunter, L., Lengauer, T. & Wodak, S., eds) pp. 314-321, AAAI Press, Menlo Park, CA.
31. Rost, B. & Sander, C. (1994) Combining evolutionary information and neural networks to predict protein secondary structure, Proteins 19, 55-77.
32. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular Cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY.
33. Sanger, F. & Coulson, A. R. (1975) A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase, J. Mol. Biol. 94, 441-448.
34. Schell, M. A. (1993) Molecular biology of the LysR family of transcriptional regulators, Annu. Rev. Microbiol. 47, 597-626.
35. Schultz, S. C., Scheilds, G. C. & Steitz, T. A. Crystal structure of a CAP-DNA complex: The DNA is bent by 90° (1991) Science 253, 1001-1007.
36. Scott, D. & Amy, N. K. (1989) Molybdenum accumulation in chlD mutants of Escherichia coli, J. Bacteriol. 171, 1284-1287.
37. Stewart, V. (1988) Nitrate respiration in relation to facultative metabolism in enterobacteria, Microbiol. Rev. 52, 190-232.
38. Stiefel, E. I. (1993) Molybdenum enzymes, cofactors and chemistry, in Molybdenum enzymes, cofactors and model systems (Stiefel, E. I., Coucouvanis, D. & Newton, W. E., eds) pp. 1-18, American Chemical Society, Washington DC.
39. Studier, F. W. & Moffatt, B. A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes, J. Mol. Biol. 189, 113-130.
40. Walkenhorst, H. M., Hemschemeier, S. K. & Eichenlaub, R. (1995) Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR,a regulatory gene, Microbiol. Res. 150. , 347-361.
41. Wang, G., Angermüller, S. & Klipp, W. (1993) Characterization of Rhodobacter capsulatus genes encoding a molybdenum transport system and putative molybdenum-pterin-binding proteins, J. Bacteriol. 175, 3031-3042.