Src protein and tyrosine-phosphorylated protein profiles in marrow stroma during osteogenic stimulation

Journal of Cellular Biochemistry

Volumn 69, Issue 3, 1998, Pages 316-325

  Klein, Benjamin Y ^a   Levitzki, Rubina ^a   Ben Bassat, Hannah ^a  

^a HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

Author keywords

Marrow stroma; Mineralization; Osteoprogenitors; Src; Tyrosine kinase dexamethasone

Indexed keywords

ALKALINE PHOSPHATASE;

ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; BONE MARROW; CONTROLLED STUDY; ENERGY METABOLISM; ENZYME ACTIVITY; FEMALE; MINERALIZATION; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT;

ANIMALS; BONE MARROW CELLS; DEXAMETHASONE; FEMALE; ONCOGENE PROTEIN PP60(V-SRC); OSTEOGENESIS; PHOSPHORYLATION; RATS; STROMAL CELLS; TEMPERATURE; TYROSINE; VALINOMYCIN;

EID: 0032104832     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(19980601)69:3<316::AID-JCB9>3.0.CO;2-U     Document Type: Article

References (26)

1. Bellows CG, Aubin JE, Heersche JNM (1987): Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro. Endocrinology 121:1985-1992.
2. Brighton CT, Hunt RM (1978): Electron microscopic pyroantimonate studies of matrix vesicles and mitochondria in the rachitic growth plate. Metab Bone Dis Relat Res 1:199-204.
3. Dannet R, Haushka RD, Aslam F, Lian J, Stein J, Stein SG (1997): Expression of cyclin-dependent kinase inhibitors are functionally related to osteoblast differentiation. J Bone Miner Res 12(Suppl):S185.
4. Goldman R, Bar-Shavit Z (1979): Dual effect of normal and stimulated macrophages and their conditioned media on target cell proliferation. J Natl Cancer Inst 63:1009-1016.
5. Hatori M, Teixeira CC, Debolt K, Pacifici M, Shapiro IM (1995): Adenine nucleotide metabolism by chondrocytes in vitro: Role of ATP in chondrocyte maturation and matrix mineralization. J Cell Physiol 165: 468-474.
6. Kamalia N, McCulloch CAG, Tenenbaum HC, Limback H (1992): Dexamethasone recruitment of self-renewing osteoprogenitor cells in chick bone marrow stromal cell culture. Blood 79:320-326.
7. Klein BY, Gal I, Segal D (1993a): Selection of malonate-resistant stromal cell-derived osteoprogenitor cells in vitro. J Cell Biochem 51:190-197.
8. Klein BY, Gal I, Segal D (1993b): Studies on the levamisole inhibitory effect on rat stromal-cell commitment to mineralization. J Cell Biochem 53:1-8.
9. Klein BY, Gal I, Hartshtark Z, Segal D (1993c): Induction of osteoprogenitor cell differentiation in rat marrow stroma increases mitochondrial retention of rhodamine 123 in stromal cells. J Cell Biochem 53:190-197.
10. Klein BY, Rojansky N, Gal I, Shlomai Z, Libergall M, Ben-Bassat H (1995): Analysis of cell-mediated mineralization in culture of bone-derived embryonic cells with neurofibromatosis. J Cell Biochem 57:530-542.
11. Klein BY, Gal I, Libergal M, Ben-Bassat H (1996a). Opposing effects on mitochondrial membrane potential by malonate and levamisole, whose effect on cell-mediated mineralization is antagonistic. J Cell Biochem 60:139-147.
12. Klein BY, Gal I, Ben-Bassat H (1996b): Cell-mediated mineralization in culture at low temperature associated with subtle thermogenic response. J Cell Biochem 63:229-238.
13. Klein BY, Tepper S, Gal I, Shlomai Z, Ben-Bassat H (1997): Opposing effects of tyrosine kinase inhibitors on mineralization of normal and tumor bone cells. J Cell Biochem 65:420-429.
14. Leboy PS, Beresford JN, Devlin C, Owen ME (1991): Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures. J Cell Physiol 146:370-378.
15. Lehninger AL (1970): Mitochondria and calcium transport. Biochem J 119:129-138.
16. Levitzki A, Gazit A (1995): Tyrosine kinase inhibition an approach to drug development. Science 267:1782-1783.
17. Lipsich LA, Lewis AJ, Brugge JS (1983): Isolation of monoclonal antibodies that recognize the transforming protein of avian sarcoma virus. J Virol 48:352-360.
18. Maniatopoulos C, Sodek J, Melcher AH (1988): Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res 254:317-330.
19. Osherov N, Levitzki A (1994): Epidermal growth factor dependent activation of src family kinases. Eur J Biochem 225:1047-1053.
20. Shapiro IM, Golub EE, Kakuta S, Hazelgrove J, Havery J, Chance B (1982): Initiation of endochondral calcification is related to changes in the redox state of hypertrophic chondrocytes. Science 217:950-952.
21. src polypeptide. Mol Cell Biol 13:3084-3092.
22. Sigal CT, Zhou W, Buser CA, McLaughlin S, Resh MD (1994): Amino-terminal basic residues of Src mediate membrane binding through electrostatic interaction with acidic phospholipids. Proc Natl Acad Sci USA 91:12253-12257.
23. Soriano P, Montgomery C, Geske R, Bradley A (1991): Targeted disruption of the c-src proto-oncogen leads to osteopetrosis in mice. Cell 64:693-702.
24. Tanaka S, Amling M, Neff L, Peyman A, Uhlmann E, Levy JB, Baron R (1996): c-Cbl is downstream of c-Src in a signaling pathway necessary for bone resorption. Nature 383:528-531.
25. Ullrich A, Schlessinger J (1990): Signal transduction by receptors with tyrosine kinase activity. Cell 61:203-212.
26. Wuthier RE (1993): Involvement of cellular metabolism of calcium and phosphate in calcification of avian growth plate cartilage. J Nutr 123:301-309.