An improved method for affinity probe localization in whole cells of filamentous fungi

Fungal Genetics and Biology

Volumn 24, Issue 1-2, 1998, Pages 3-13

  Bourett, Timothy M ^a   Czymmek, Kirk J ^b   Howard, Richard J ^a  

^a DUPONT COMPANY   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

Author keywords

Butyl methyl methacrylate; Confocal microscopy; Cytoskeleton; Endomembrane system; Freeze substitution; Immunofluorescence; Lectin labeling; Microtubules; Spitzenkorper; Tip growth

Indexed keywords

ALPHA TUBULIN; BETA TUBULIN; CONCANAVALIN A; FUNGUS ANTIBODY; LECTIN; METHACRYLIC ACID;

ANTIBODY COMBINING SITE; APICAL MEMBRANE; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL ORGANELLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FREEZE DRYING; FUNGAL GENETICS; FUNGUS HYPHAE; GOLGI COMPLEX; IMAGING; MICROTUBULE; MITOSIS; MOLECULAR WEIGHT; MYCELIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING;

FUNGI;

EID: 0032096337     PISSN: 10871845     EISSN: None     Source Type: Journal    
DOI: 10.1006/fgbi.1998.1054     Document Type: Article

References (45)

1. Baskin T. I., Busby C. H., Fowke L. C., Sammut M., Gubler F. Improvements in immunostaining samples embedded in methacrylate: Localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. Planta. 187:1992;405-413.
2. Baskin T. I., Miller D. D., Vos J. W., Wilson J. E., Hepler P. K. Cryofixing single cells and multicellular specimens enhances structure and immunocytochemistry for light microscopy. J. Microsc. 182:1996;149-161.
3. Bourett T. M., Hoch H. C., Staples R. C. Association of the microtubule cytoskeleton with the thigmotropic signal for appressorium formation inUromyces. Mycologia. 79:1987;540-545.
4. Bourett T. M., Howard R. J. In vitro development of penetration structures in the rice blast fungus,Magnaporthe grisea. Can. J. Bot. 68:1990;329-342.
5. Bourett T. M., Howard R. J. Ultrastructural immunolocalization of actin in a fungus. Protoplasma. 163:1991;199-202.
6. Bourett T. M., Howard R. J. Actin in penetration pegs of the fungal rice blast pathogen,Magnaporthe grisea. Protoplasma. 168:1992;20-26.
7. Bourett T. M., Howard R. J. Enhanced labelling of concanavalin A binding sites in fungal endomembranes using a double-sided, indirect method. Mycol. Res. 98:1994;769-775.
8. Bourett T. M., Picollelli M. A., Howard R. J. Postembedment labeling of intracellular Concanavalin A-binding sites in freeze-substituted fungal cells. Exp. Mycol. 17:1993;223-235.
9. Cochrum K. R. Immunocytochemistry. Review of Physiological Chemistry. 1977;Lange, Los Altos. p. 596-608.
10. Czymmek K. J., Wallon J. H., Klomparens K. L. Confocal microscopy in mycological research. Exp. Mycol. 18:1994;275-293.
11. Czymmek K. J., Bourett T. M., Howard R. J. Immunolocalization of tubulin and actin in thick-sectioned fungal hyphae after freeze-substitution and methacrylate de-embedment. J. Microsc. 181:1996;153-161.
12. Derksen J., Emmons A. M. Microtubules in tip growth systems. Heath I. B. Tip Growth in Plant and Fungal Cells. 1990;147-181 Academic Press, San Diego.
13. Girbardt M. Die ultrastruktur der Apikalregion von Pilzhyphen. Protoplasma. 67:1969;413-441.
14. Glauert A. M. Embedding. Glauert A. M. Fixation, Dehydration and Embedding of Biological Specimens. 1975;123-176 North-Holland, Amsterdam.
15. Grove S. N., Bracker C. E. Protoplasmic organization of hyphal tips among fungi: Vesicles and Spitzenkörper. J. Bacteriol. 104:1970;989-1009.
16. Gubler F. Immunofluorescence localisation of microtubules in plant root tips embedded in butyl-methyl methacrylate. Cell Biol. Int. Rep. 13:1989;137-145.
17. Gunderson J. H., Elwood H., Ingold A., Kindle K., Sogin M. L. Phylogenetic relationships between chlorophytes, chrysophytes, and oomycetes. Proc. Natl. Acad. Sci. USA. 84:1987;5823-5827.
18. Harris P. J., Rubin B. P. Transition from mitosis to interphase in sea urchin first division: Immunofluorescent studies of tubulin distribution in methacrylate sections. J. Histochem. Cytochem. 35:1987;343-349.
19. Heath I. B., Kaminskyj S. G. W. The organization of tip-growth-related organelles and microtubules revealed by quantitative analysis of freeze-substituted oömycete hyphae. J. Cell Sci. 93:1989;41-52.
20. Hippe S., Hermanns M. Improved structural preservation in freeze-substituted sporidia ofUstilago avenae. Protoplasma. 135:1986;19-30.
21. Hoch H. C. Freeze-substitution of fungi. Aldrich H. C., Todd W. J. Ultrastructure Techniques for Microorganisms. 1986;183-212 Plenum, New York.
22. Hoch H. C., Staples R. C. Ultrastructural organization of the non-differentiated uredospore germling ofUromyces phaseolitypica. Mycologia. 75:1983;795-824.
23. Hoch H. C., Staples R. C. The microtubule cytoskeleton in hyphae ofUromyces phaseoli. Protoplasma. 124:1985;112-122.
24. Howard R. J. Ultrastructural analysis of hyphal tip cell growth in fungi: Spitzenkörper, cytoskeleton and endomembranes after freeze-substitution. J. Cell Sci. 48:1981;89-103.
25. Howard R. J., Aist J. R. Hyphal tip cell ultrastructure of the fungusFusarium: J. Ultrastruct. Res. 66:1979;224-234.
26. Howard R. J., Aist J. R. Cytoplasmic microtubules and fungal morphogenesis: Ultrastructural effects of MBC determined by freeze-substitution of hyphal tip cells. J. Cell Biol. 87:1980;55-64.
27. Howard R. J., O'Donnell K. L. Freeze substitution of fungi for cytological analysis. Exp. Mycol. 11:1987;250-269.
28. Humbel B., Marti T., Müller M. Improved structural preservation by combining freeze substitution and low temperature embedding. Beitr. Elecktronenmikroskop. Direktabb. Oberfl. 16:1983;585-594.
29. Hyde G. J., Hardham A. R. Confocal microscopy of microtubule arrays in cryosectioned sporangia ofPhytophthora cinnamomi. Exp. Mycol. 16:1992;207-218.
30. Kaminskyj S. G. W., Jackson S. L., Heath I. B. Fixation induces differential polarized translocations of organelles in hyphae ofSaprolegnia ferax. J. Microsc. 167:1992;153-168.
31. Keller H. E. Objective lenses for confocal microscopy. Pawley J. B. Handbook of Biological Confocal Microscopy. 1995;111-126 Plenum, New York.
32. Kwon Y. H., Wells K. S., Hoch H. C. Fluorescence confocal microscopy: Applications in fungal cytology. Mycologia. 85:1993;721-733.
33. Lloyd C. W., Wells B. Microtubules are at the tips of root hairs and form helical patterns corresponding to inner wall fibrils. J. Cell Sci. 75:1985;225-238.
34. López-Franco R., Bracker C. E. Diversity and dynamics of the Spitzenkörper in growing hyphal tips of higher fungi. Protoplasma. 195:1996;90-111.
35. Lowry D. S., Roberson R. W. Microtubule organization during zoosporogenesis inAllomyces macrogynus. Protoplasma. 196:1997;45-54.
36. McKerracher L. J., Heath I. B. Cytoplasmic migration and intracellular organelle movements during tip growth of fungal hyphae. Exp. Mycol. 11:1987;79-100.
37. Nicolas M.-T., Bassot J.-M. Freeze substitution after fast-freeze fixation in preparation for immunocytochemistry. Microsc. Res. Tech. 24:1993;474-487.
38. Nicolas M.-T., Bassot J.-M., Nicolas G. Immunogold labeling of luciferase in the luminous bacteriumVibrio harveyi. J. Histochem. Cytochem. 37:1989;663-674.
39. Raudaskoski M., Rupes I., Timonen S. Immunofluorescence microscopy of the cytoskeleton in filamentous fungi after quick-freezing and low-temperature fixation. Exp. Mycol. 15:1991;167-173.
40. Roberson R. W. The hyphal tip cell ofSclerotium rolfsii: NATO ASI Ser. H. 53:1991;27-37.
41. Roberson R. W. The actin cytoskeleton in hyphal cells ofSclerotium rolfsii. Mycologia. 84:1992;41-51.
42. Roberson R. W., Fuller M. S., Grabski C. Effects of the demethylase inhibitor, cyproconazole, on hyphal tip cells ofSclerotium rolfsii. Pest. Biochem. Physiol. 34:1989;130-142.
43. Roberson R. W., Vargas M. M. The tubulin cytoskeleton and its sites of nucleation in hyphal tips ofAllomyces macrogynus. Protoplasma. 182:1994;19-31.
44. Salo V., Niini S. S., Virtanen I., Raudaskoski M. Comparative immunocytochemistry of the cytoskeleton in filamentous fungi and dikaryotic and multinucleate hyphae. J. Cell Sci. 94:1989;11-24.
45. Srinivasan S., Vargas M. M., Roberson R. W. Functional, organizational, and biochemical analysis of actin in hyphal tip cells ofAllomyces macrogynus. Mycologia. 88:1996;57-70.