Cryofixing single cells and multicellular specimens enhances structure and immunocytochemistry for light microscopy

Journal of Microscopy

Volumn 182, Issue 2, 1996, Pages 149-161

  Baskin, T I ^a   Miller, D D ^b,c   Vos, J W ^b   Wilson, J E ^a   Hepler, P K ^b  

^a UNIVERSITY OF MISSOURI   (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Actin; Chemical fixation; Cryofixation; Immunocytochemistry; Methacrylate; Microtubules; Plunge freezing; Pollen tubes; Removable embedment; Roots; Stamen hairs

Indexed keywords

ANTIGENS; CELLS; FREEZING; MICROSCOPES; PROTEINS;

ACTIN; CHEMICAL FIXATION; CRYOFIXATION; IMMUNOCYTOCHEMISTRY; METHACRYLATE; MICROTUBULES; PLUNGE FREEZING; POLLEN TUBE; REMOVABLE EMBEDMENT; ROOT; STAMEN HAIR;

CYTOLOGY;

ACTIN;

ARTICLE; IMMUNOCYTOCHEMISTRY; MICROSCOPY; MICROTUBULE; PLANT; PRIORITY JOURNAL;

ARABIDOPSIS THALIANA; LILIUM LONGIFLORUM; NICOTIANA ALATA; TRADESCANTIA;

EID: 0030048459     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1996.135417.x     Document Type: Article

References (44)

1. Andersland, J., Fisher, D., Wymer, C., Cyr, R. & Parthasarathy, M. (1994) Characterization of a monoclonal antibody prepared against plant actin. Cell Motil. Cytoskel. 29, 339-344.
2. Angerer, L.M. & Angerer, R.C. (1991) Localization of mRNAs by in situ hybridization. Functional Organization of the Nucleus: a laboratory Guide. Methods in Cell Biology, Vol. 35 (ed. by B. A. Hamalko & S. C. R. Elgin), pp. 57-71. Academic Press, San Diego.
3. Baskin, T.I. & Bivens, N.J. (1995) Stimulation of radial expansion in arabidopsis roots by inhibitors of actomyosin and vesicle secretion but not by various inhibitors of metabolism. Planta. 197, 514-521.
4. Baskin, T.I., Busby, C.H., Fowke, L.C., Sammut, M. & Gubler, F. (1992) Improvements in immunostaining samples embedded in methacrylate: Localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. Planta, 187, 405-413.
5. Baskin, T.I., Wilson, J.E., Cork, A. & Williamson, R.E. (1994) Morphology and microtubule organization in Arabidopsis roots exposed to oryzalin or taxol. Plant Cell Physiol. 35, 935-944.
6. Czymmek, K.J., Bourett, T.M. & Howard, R.J. (1996) Immunolocalization of tubulin and actin in thick-sectioned fungal hyphae after freeze substitution fixation and methacrylate de-embedment. J. Microsc. 181 (in press).
7. Dahl, R. & Staehelin, L.A. (1989) High-pressure freezing for the preservation of biological structure: theory and practise. J. Electron Microsc. Tech. 13, 165-174.
8. Ding, B., Turgeon, R. & Parthasarathy, M.V. (1991) Routine cryofixation of plant tissue by propane jet freezing for freeze substitution. J. Electron Microsc. Tech. 19, 107-117.
9. Feder, N. & Sidman, R.L. (1958) Methods and principles of fixation by freeze substitution. J. Biophys. Biochem. Cytol. 4, 593-602.
10. Gilkey, J.C. & Staehelin, L.A. (1986) Advances in ultrarapid freezing for the preservation of cellular ultrastructure. J. Electron Microsc. Tech. 3, 177-210.
11. Gubler, F. (1989) Immunofluorescence localization of microtubules in plant root tips embedded in butyl-methyl methacrylate. Cell Biol. Int. Rep. 13, 137-145.
12. Harvey, D.M.R. (1982) Freeze-substitution. J. Microsc. 127, 209-221.
13. He, Y. & Wetzstein, H.Y. (1995) Fixation induces differential tip morphology and immunolocalization of the cytoskeleton in pollen tubes. Physiol. Plant. 93, 757-763.
14. Hepler, P.K., Cleary, A.L., Gunning, B.E.S., Wadsworth, P., Wasteneys, G.O. & Zhang, D.H. (1993): Cytoskeletal dynamics in living plant cells. Cell Biol. Int. Rep. 17, 127-142.
15. Hippe-Sanwald, S. (1993) Impact of freeze substitution on biological electron microscopy. Microsc. Res. Tech. 24, 400-422.
16. Howard, R.J. & O'Donnell, K.L. (1987) Freeze substitution of fungi for cytological analysis. Exp. Mycol. 11, 250-269.
17. Hush, J.M., Wadsworth, P., Callaham, D.A. & Hepler, P.K. (1994) Quantification of microtubule dynamics in living plant cells using fluorescence redistribution after photobleaching. J. Cell Sci. 107, 775-784.
18. Hyde, G.J., Lancelle, S., Hepler, P.K. & Hardham, A.R. (1991) Sporangium structure in Phytophthora is disrupted after high pressure freezing. Protoplasma, 165, 203-208.
19. Kaeser, W. (1989) Freeze-substitution of plant tissues with a new medium containing dimethoxypropane. J. Microsc. 154, 273-278.
20. Kaeser, W., Koyro, H.-W. & Moor, H. (1989) Cryofixation of plant tissues without pretreatment. J. Microsc. 154, 279-288.
21. Kiss, J.Z. & McDonald, K. (1993) Electron microscopy immunocytochemistry following cryofixation and freeze substitution. Antibodies in Cell Biology. Methods in Cell Biology, Vol. 37 (ed. by D. J. Asai), pp. 311-341. Academic Press, San Diego.
22. Lancelle, S.A., Callaham, D.A. & Hepler, P.K. (1986) A method for rapid freeze fixation of plant cells. Protoplasma, 131, 153-165.
23. Lancelle, S.A., Cresti, M. & Hepler, P.K. (1987) Ultrastructure of the cytoskeleton in freeze-substituted pollen tubes of Nicotiana alata. Protoplasma, 140, 141-150.
24. Lancelle, S.A. & Hepler, P.K. (1991) Association of actin with cortical microtubules revealed by immunogold localization in Nicotiana pollen tubes. Protoplasma, 165, 167-172.
25. Lancelle, S.A. & Hepler, P.K. (1992) Ultrastructure of freeze-substituted pollen tubes of Lilium longiflorum. Protoplasma, 167, 215-230.
26. McCully, M.E. & Canny, M.J. (1985) The stabilization of labile configurations of plant cytoplasm by freeze-substitution. J. Microsc. 139, 27-33.
27. Melan, M.A. & Sluder, G. (1992): Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluorescence microscopy. J. Cell Sci. 101, 731-743.
28. Menco, B.Ph.M. (1986) A survey of ultra-rapid cryofixation methods with particular emphasis on applications to freeze-fracturing, freeze-etching and freeze substitution. J. Electron Micros. Tech. 4, 177-240.
29. Mersey, B. & McCully, M.E. (1978) Monitoring the course of fixation of plant cells. J. Microsc. 114, 49-76.
30. Miller, D.D., Scordilis, S.P. & Hepler, P.K. (1995) Identification and localization of three classes of myosins in pollen tubes of Lilium longiflorum and Nicotiana alata. J. Cell Sci. 108, 2549-2563.
31. Monaghan, P. & Robertson, D. (1990) Freeze-substitution without aldehyde or osmium fixatives: ultrastructure and implications for immunocytochemistry. J. Microsc. 153, 355-363.
32. Nicolas, M.-T. & Bassot, J.-M. (1993) Freeze substitution after fast-freeze fixation in preparation for immunocytochemistry. Microsc. Res. Tech. 24, 474-487.
33. Phillips, T.E. & Boyne, A.F. (1984) Liquid nitrogen-based quick freezing: experiences with bounce-free delivery of cholinergic nerve terminals to a metal surface. J. Electron Microsc. Tech. 1, 9-29.
34. Quintana, C. (1994) Cryofixation, cryosubstitution, cryoembedding for ultrastructural, immunocytochemical and microanalytical studies. Micron, 25, 63-99.
35. Raudaskoski, M., Rupeš, I. & Timonen, S. (1991) Immunofluorescence microscopy of the cytoskeleton in filamentous fungi after quick-freezing and low temperature fixation. Exp. Mycol. 15, 167-173.
36. Ridge, R.W. (1990) A simple apparatus and technique for the rapid-freeze and freeze-substitution of single-cell algae. J. Electron Microsc. 39, 120-124.
37. Roberson, R.W. & Vargas, M.M. (1994) The tubulin cytoskeleton and its sites of nucleation in hyphal tips of Allomyces macrogynus. Protoplasma, 182, 19-31.
38. Ryan, K.P. (1992) Cryofixation of tissues for electron microscopy: A review of plunge freeze cooling methods. Scanning Microsc. 6, 715-743.
39. Shopfer, C.R. & Hepler, P.K. (1991) Distribution of membranes and the cytoskeleton during cell plate formation in pollen mother cells of Tradescantia. J. Cell Sci. 100, 717-728.
40. Sitte, H., Edelmann, L. & Neumann, K. (1987) Cryofixation without pretreatment at ambient pressure. Cryotechniques in Biological Electron Microscopy (ed. by R. A. Steinbrecht & K. Zierold), pp. 87-111. Springer, Berlin.
41. Skaer, R.J. & Whytock, S. (1976) The fixation of nuclei and chromosomes. J. Cell Sci. 20, 221-231.
42. Tiwari, S.C. & Polito, V.S. (1988) Organization of the cytoskeleton in pollen tubes of Pyrus communis: a study employing conventional and freeze-substitution electron microscopy, immunofluorescence and rhodamine-phalloidin. Protoplasma, 147, 100-112.
43. Van de Kant, H.J.G., Boon, M.E. & De Rooij, D.G. (1988) Microwave-aided technique to detect bromodeoxyuridine in S-phase cells using immunogold-silver staining and plastic-embedded sections. Histochem. J. 20, 335-340.
44. Yuan, M., Shaw, P.J., Warn, R.M. & Lloyd, C.W. (1994) Dynamic reorientation of cortical microtubules, from transverse to longitudinal, in living plant cells. Proc. Natl. Acad. Sci. USA, 91, 6050-6053.