A single amino acid change in the yeast retrotransposon Ty5 abolishes targeting to silent chromatin

Molecular Cell

Volumn 1, Issue 7, 1998, Pages 1051-1055

  Gai, Xiaowu ^a   Voytas, Daniel F ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE; RNA DIRECTED DNA POLYMERASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CHROMATIN; CHROMOSOME; GENE EXPRESSION REGULATION; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; MOLECULAR GENETICS; POINT MUTATION; RETROPOSON; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; TRANSPOSON;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CHROMATIN; CHROMOSOMES, FUNGAL; DNA TRANSPOSABLE ELEMENTS; INTEGRASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; POINT MUTATION; RECOMBINATION, GENETIC; RETROELEMENTS; RNA-DIRECTED DNA POLYMERASE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032086911     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)80105-7     Document Type: Article

References (24)

1. Abraham, J., Nasmyth, K.A., Strathern, J.N., Klar, A.J.S., and Hicks, J.B. (1984). Regulation of mating-type information in yeast: negative control requiring sequences both 5′ and 3′ to the regulated region. J. Mol. Biol. 176, 307-331.
2. Chalker, D.L., and Sandmeyer, S.B. (1993). Sites of RNA polymerase III transcription initiation and Ty3 integration at the U6 gene are positioned by the TATA box. Proc. Natl. Acad. Sci. USA 90, 4927-4931.
3. Chalker, D.L., and Sandmeyer, S.B. (1992). Ty3 integrates within the region of RNA polymerase III transcription initiation. Genes Dev. 6, 117-128.
4. Curcio, M.J., and Morse, R.H. (1996). Tying together integration and chromatin. Trends Genet. 12, 436-438.
5. Devine, S.E., and Boeke, J.D. (1996). Regionally-specific, targeted integration of the yeast retrotransposon Ty1 upstream of genes transcribed by RNA polymerase III. Genes Dev. 10, 620-633.
6. Gallay, P., Hope, T., Chin, D., and Trono, D. (1997). HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway. Proc. Natl. Acad. Sci. USA 94, 9825-9830.
7. Kalpana, G.V., Marmon, S., Wang, W., Crabtree, G.R., and Goff, S.P. (1994). Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. Science 266, 2002-2006.
8. Katz, R.A., and Skalka, A.M. (1994). The retroviral enzymes. Annu. Rev. Biochem. 63, 133-173.
9. Keeney, J.B., Chapman, K.B., Lauermann, V., Voytas, D.F., Astrom, S.U., von Pawel-Rammingen, U., Bystrom, A., and Boeke, J.D. (1995). Multiple molecular determinants for retrotransposition in a primer tRNA. Mol. Cell. Biol. 15, 217-226.
10. Kenna, M.A., Brachmann, C.B., Devine, S.E., and Boeke, J.D. (1997). Invading the yeast nucleus: a nuclear localization signal and the C-terminus of Ty1 integrase is required for transposition in vivo. Mol. Cell. Biol. 18, 1115-1124.
11. Kimmerly, W.J., and Rine, J. (1987). Replication and segregation of plasmids containing cis-acting regulatory sites of silent mating-type genes in Saccharomyces cerevisiae are controlled by the SIR genes. Mol. Cell. Biol. 7, 4225-4237.
12. Kirchner, J., Connolly, C.M., and Sandmeyer, S.B. (1995). Requirement of RNA polymerase III transcription factors for in vitro position-specific integration of a retrovirus-like element. Science 267, 1488-1491.
13. Laurenson, P., and Rine, J. (1992). Silencers, silencing, and heritable transcriptional states. Microbiol. Rev. 56, 543-560.
14. Moore, S.P., and Garfinkel, D.J. (1994). Expression and partial purification of enzymatically active recombinant Ty1 integrase in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 91, 1843-1847.
15. Moore, S.P., Rinckel, L.A., and Garfinkel, D.J. (1997). A Ty1 integrase nuclear localization signal required for retrotransposition. Mol. Cell. Biol. 18, 1105-1114.
16. Palladino, F., Laroche, T., Gilson, E., Axelrod, A., Pillus, L., and Gasser, S.M. (1993). SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres. Cell 75, 543-555.
17. Sandmeyer, S.B., Hansen, L.J., and Chalker, D.L. (1990). Integration specificity of retrotransposons and retroviruses. Annu. Rev. Genet. 24, 491-518.
18. Sikorski, R.S., and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
19. Thompson, J.D., Higgins, D.G., and Gibson, T.J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
20. Xiong, Y., and Eickbush, T.H. (1990). Origin and evolution of retroelements based upon their reverse transcriptase sequences. EMBO J. 9, 3353-3362.
21. Zou, S., and Voytas, D.F. (1997). Silent chromatin determines target preference of the retrotransposon Ty5. Proc. Natl. Acad. Sci. USA 94, 7412-7416.
22. Zou, S., Wright, D.A., and Voytas, D.F. (1995). The Saccharomyces Ty5 retrotransposon family is associated with origins of DNA replication at the telomeres and the silent mating locus HMR. Proc. Natl. Acad. Sci. USA 92, 920-924.
23. Zou, S., Ke, N., Kim, J.M., and Voytas, D.F. (1996a). The Saccharomyces retrotransposon Ty5 integrates preferentially into regions of silent chromatin at the telomeres and mating loci. Genes Dev. 10, 634-645.
24. Zou, S., Kim, J.M., and Voytas, D.F. (1996b). The Saccharomyces retrotransposon Ty5 influences the organization of chromosome ends. Nucleic Acids Res. 24, 4825-4831.