Genomic organization of the rat connexin40 gene: Identical transcription start sites in heart and lung

Cardiovascular Research

Volumn 38, Issue 2, 1998, Pages 463-471

  Groenewegen, W Antoinette ^a   Van Veen, Toon A B ^a   Van Der Velden, Huub M W ^a   Jongsma, Habo J ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Connexin40; Developmental; Expression; Gap junctions; Gene; Promoter; Rat; Regulation; Tissue specific

Indexed keywords

CONNEXIN 40; DNA; GAP JUNCTION PROTEIN; MESSENGER RNA; UNCLASSIFIED DRUG;

ADOLESCENT; ANIMAL CELL; ANIMAL TISSUE; AORTA MEDIA; ARTICLE; CONTROLLED STUDY; EXON; GENE SEQUENCE; HEART ATRIUM; HEART VENTRICLE; INTRON; LUNG; MALE; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; RAT; SMOOTH MUSCLE FIBER; TRANSCRIPTION INITIATION;

AGING; ANIMALS; BASE SEQUENCE; CONNEXINS; EXONS; GAP JUNCTIONS; LUNG; MALE; MOLECULAR SEQUENCE DATA; MYOCARDIUM; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RATS; RATS, WISTAR; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 0032076485     PISSN: 00086363     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0008-6363(97)00325-8     Document Type: Article

References (32)

1. [1] Gros DB, Jongsma HJ. Connexins in mammalian heart function. Bioessays 1996;18:719-730.
2. [2] Bastide B, Neyses L, Ganten D, Paul M, Willecke K, Traub O. Gap junction protein connexin40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. Circ Res 1993;73:1138-1149.
3. [3] Beyer EC, Reed KE, Westphale EM, Kanter HL, Larson DM. Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle. J Membr Biol 1992;127:69-76.
4. [4] Haefliger JA, Bruzzone R, Jenkins NA, Gilbert DJ, Copeland NG, Paul DL. Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping. J Biol Chem 1992;267:2057-2064.
5. [5] Van Kempen MJ, Vermeulen JL, Moorman AF, Gros D, Paul DL, Lamers WH. Developmental changes of connexin40 and connexin43 mRNA distribution patterns in the rat heart. Cardiovasc Res 1996;32:886-900.
6. [6] Neuhaus IM, Dahl G, Werner R. Use of alternate promoters for tissue-specific expression of the gene coding for connexin32. Gene 1995;158:257-262.
7. [7] Sohl G, Gillen C, Bosse F, Gleichmann M, Muller HW, Willecke K. A second alternative transcript of the gap junction gene connexin32 is expressed in murine Schwann cells and modulated in injured sciatic nerve. Eur J Cell Biol 1996;69:267-275.
8. [8] Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
9. [9] Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA. An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res 1995;23:1087-1088.
10. [10] Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. Current Protocols in Molecular Biology, 1997, pp. 4.8.1-4.8.5.
11. [11] Hennemann H, Suchyna T, Lichtenberg Frate H, Jungbluth S, Dahl E, Schwarz J, Nicholson BJ, Willecke K. Molecular cloning and functional expression of mouse connexin40, a second gap junction gene preferentially expressed in lung. J Cell Biol 1992;117:1299-1310.
12. [12] Rivera VM, Sheng M, Greenberg ME. The inner core of the serum response element mediates both the rapid induction and subsequent repression of c-fos transcription following serum stimulation. Genes Dev 1990;4:255-268.
13. [13] Mably JD, Sole MJ, Liew CC. Characterization of the GArC motif. A novel cis-acting element of the human cardiac myosin heavy chain genes. J Biol Chem 1993;268:476-482.
14. [14] Chen CY, Schwartz RJ. Identification of novel DNA binding targets and regulatory domains of a murine tinman homeodomain factor, Nkx-2.5.. J Biol Chem 1995;270:15628-15633.
15. [15] Lenardo MJ, Baltimore D. NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control. Cell 1989;58:227-229.
16. [16] Kanter HL, Saffitz JE, Beyer EC. Cardiac myocytes express multiple gap junction proteins. Circ Res 1992;70:438-444.
17. [17] Kanter HL, Saffitz JE, Beyer EC. Molecular cloning of two human cardiac gap junction proteins, connexin40 and connexin45. J Mol Cell Cardiol 1994;26:861-868.
18. [18] Miller T, Dahl G, Werner R. Structure of a gap junction gene: rat connexin-32. Biosci Rep 1988;8:455-464.
19. [19] Bai S, Spray DC, Burk RD. Identification of proximal and distal regulatory elements of the rat connexin32 gene. Biochim Biophys Acta 1993;1216:197-204.
20. [20] Hennemann H, Kozjek G, Dahl E, Nicholson B, Willecke K. Molecular cloning of mouse connexins26 and -32: similar genomic organization but distinct promoter sequences of two gap junction genes. Eur J Cell Biol 1992;58:81-89.
21. [21] Yu W, Dahl G, Werner R. The connexin43 gene is responsive to oestrogen. Proc R Soc Lond B Biol Sci 1994;255:125-132.
22. [22] Sullivan R, Ruangvoravat C, Joo D, Morgan J, Wang BL, Wang XK, Lo CW. Structure, sequence and expression of the mouse Cx43 gene encoding connexin 43. Gene 1993;130:191-199.
23. [23] Pugh BF, Tjian R. Mechanism of transcriptional activation by Sp1: evidence for coactivators. Cell 1990;61:1187-1197.
24. [24] Mitchell PJ, Wang C, Tjian R. Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell 1987;50:847-861.
25. [25] Mably JD, Liew CC. Factors involved in cardiogenesis and the regulation of cardiac-specific gene expression. Circ Res 1996;79:4-13.
26. [26] Srivastava D, Cserjesi P, Olson EN. A subclass of bHLH proteins required for cardiac morphogenesis. Science 1995;270:1995-1999.
27. [27] Delorme B, Dahl E, Jarry-Guichard T, Brand J, Willecke K, Gros D, Theveniau-Ruissy M. Expression pattern of connexin gene products at the early developmental stages of the mouse cardiovascular system. Circ Res 1997;81:423-437.
28. [28] Durocher D, Chen CY, Ardati A, Schwartz RJ, Nemer M. The atrial natriuretic factor promoter is a downstream target for Nkx-2.5 in the myocardium. Mol Cell Biol 1996;16:4648-4655.
29. [29] Chen CY, Schwartz RJ. Recruitment of the tinman homolog Nkx-2.5 by serum response factor activates cardiac alpha-actin gene transcription. Mol Cell Biol 1996;16:6372-6384.
30. [30] Osborn L, Kunkel S, Nabel GJ. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B. Proc Natl Acad Sci USA 1989;86:2336-2340.
31. [31] Mensink A, de Haan LH, Lakemond CM, Koelman CA, Koeman JH. Inhibition of gap junctional intercellular communication between primary human smooth muscle cells by tumor necrosis factor alpha. Carcinogenesis 1995;16:2063-2067.
32. [32] Van Rijen HVM, Van Kempen MJA, Postma S, Jongsma HJ. Tumor Necrosis Factor-α alters the expression of Connexin43, Connexin40, and Connexin37 in human umbilical vein endothelial cells. Cytokine; in press.