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of special interest. This paper presents the most detailed structural analysis of a regional centromere yet described. The 420kb centromere of Dp1187 is composed primarily of various short satellites and single copies of retrotransposable elements that account for the 'islands' of complex DNA reported in [26]. Notably, the satellites and the transposable elements are neither specific to centromeres within the Drosophila genome nor are they found at all centromeres. Thus the nature of any DNA signal for centromere formation is unclear.
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Sun X, Wahlstrom J, Karpen G. Molecular structure of a functional Drosophila centromere. of special interest Cell. 91:1997;1007-1019 This paper presents the most detailed structural analysis of a regional centromere yet described. The 420kb centromere of Dp1187 is composed primarily of various short satellites and single copies of retrotransposable elements that account for the 'islands' of complex DNA reported in [26]. Notably, the satellites and the transposable elements are neither specific to centromeres within the Drosophila genome nor are they found at all centromeres. Thus the nature of any DNA signal for centromere formation is unclear.
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Sun, X.1
Wahlstrom, J.2
Karpen, G.3
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9
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Characterization of neo-centromeres in marker chromosomes lacking detectable alpha satellite DNA
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Depinet TW, Zackowski JL, Earnshaw WC, Kaffe S, Sekhon GS, Stallard R, Sullivan BA, Vance BH, Van Dyke DL, Willard HF, et al. Characterization of neo-centromeres in marker chromosomes lacking detectable alpha satellite DNA. Hum Mol Genet. 6:1997;1195-1204.
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Depinet, T.W.1
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10
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0345293849
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A functional neocentromere formed through activation of a talent human centromere and consisting on non-alpha satellite DNA
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of special interest. A very thorough structural and cytological analysis of a human neo-centromere that maps the functional neo-centromere to a ≈80kb region from chromosome 10q25 that appears indistinguishable from the same DNA on a normal chromosome 10. No sequence data are presented for the region, which is stated to be free of alpha satellite DNA or other satellite DNAs.
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du Sart D, Cancilla MR, Earle E, Mao J, Saffery R, Tainton KM, Kalitsis P, Martyn J, Barry AE, Choo KHA. A functional neocentromere formed through activation of a talent human centromere and consisting on non-alpha satellite DNA. of special interest Nat Genet. 16:1997;144-153 A very thorough structural and cytological analysis of a human neo-centromere that maps the functional neo-centromere to a ≈80kb region from chromosome 10q25 that appears indistinguishable from the same DNA on a normal chromosome 10. No sequence data are presented for the region, which is stated to be free of alpha satellite DNA or other satellite DNAs.
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Nat Genet
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Du Sart, D.1
Cancilla, M.R.2
Earle, E.3
Mao, J.4
Saffery, R.5
Tainton, K.M.6
Kalitsis, P.7
Martyn, J.8
Barry, A.E.9
Choo, K.H.A.10
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0031437950
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The case for epigenetic effects on centromere identity and function
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of special interest. A very insightful and provocative recent review which summarizes the evidence that centromeres are subject to epigenetic effects, including activation and inactivation.
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Karpen GH, Allshire RC. The case for epigenetic effects on centromere identity and function. of special interest Trends Genet. 13:1997;489-496 A very insightful and provocative recent review which summarizes the evidence that centromeres are subject to epigenetic effects, including activation and inactivation.
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Karpen, G.H.1
Allshire, R.C.2
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12
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0030910465
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Formation of de novo centromeres and construction of first-generation human artificial microchromosomes
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of special interest. The first report of the formation of human artificial chromosomes formed by combining individually isolated structural components of human chromosomes.
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Harrington JJ, Van Bokkelen G, Mays RW, Gustashaw K, Willard HF. Formation of de novo centromeres and construction of first-generation human artificial microchromosomes. of special interest Nat Genet. 15:1997;345-355 The first report of the formation of human artificial chromosomes formed by combining individually isolated structural components of human chromosomes.
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Harrington, J.J.1
Van Bokkelen, G.2
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Willard HF. Centromeres of mammalian chromosomes. Trends Genet. 6:1990;410-416.
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Willard, H.F.1
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Evolution of centromeric alpha satellite DNA: Molecular organization within and between human and primate chromosomes
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M. Jackson, T. Strachan, Dover G. Oxford: BIOS Scientific Publishers
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Warburton PE, Willard HF. Evolution of centromeric alpha satellite DNA: molecular organization within and between human and primate chromosomes. Jackson M, Strachan T, Dover G. Human Genome Evolution. 1996;121-145 BIOS Scientific Publishers, Oxford.
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Human Genome Evolution
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Physical and genetic mapping of the human X chromosome centromere: Repression of recombination
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Mahtani MM, Willard HF. Physical and genetic mapping of the human X chromosome centromere: repression of recombination. Genome Res. 8:1998;100-110.
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Laurent AM, Puechberty J, Prades C, Gimenez S, Roizes G. Site-specific retrotransposition of L1 elements within human alphoid satellite sequences. Genomics. 46:1997;127-132.
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Round EK, Flowers SK, Richards EJ. Arabidopsis thaliana centromere regions: genetic map positions and repetitive DNA structure. Genome Res. 7:1997;1045-1053.
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Round, E.K.1
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Haaf T, Warburton PE, Willard HF. Integration of human alpha satellite DNA into simian chromosomes: centromere protein binding and disruption of normal chromosome segregation. Cell. 70:1992;681-696.
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Haaf, T.1
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Larin Z, Fricker MD, Tyler-Smith C. De novo formation of several features of a centromere following introduction of a Y alphoid YAC into mammalian cells. Hum Mol Genet. 3:1994;689-695.
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Larin, Z.1
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0030885531
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Hamster chromosomes containing amplified human alpha satellite DNA show delayed sister chromatid separation in the absence of de novo kinetochore formation
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Warburton PE, Cooke HJ. Hamster chromosomes containing amplified human alpha satellite DNA show delayed sister chromatid separation in the absence of de novo kinetochore formation. Chromosoma. 106:1997;149-159.
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Warburton, P.E.1
Cooke, H.J.2
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Dissecting the centromere of the human Y chromosome with cloned telomeric DNA
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Brown KE, Barnett MA, Burgtorf C, Shaw P, Buckle VJ, Brown WRA. Dissecting the centromere of the human Y chromosome with cloned telomeric DNA. Hum Mol Genet. 3:1994;1227-1237.
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Brown, K.E.1
Barnett, M.A.2
Burgtorf, C.3
Shaw, P.4
Buckle, V.J.5
Brown, W.R.A.6
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Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation
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Farr CJ, Bayne RA, Kipling D, Mills W, Critcher R, Cooke HJ. Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation. EMBO J. 14:1995;5444-5454.
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Farr, C.J.1
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Cooke, H.J.6
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24
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0029908609
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Minichromosomes derived from the human Y chromosome by telomere-directed chromosome breakage
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of special interest. This paper reports the latest in a series of elegant experiments to dissect the centromere of the human Y chromosome. Several rounds of telomere-directed chromosome breakage resulted in the generation of small derivatives of the Y that range in size from ≈2.5 to 8 Mb. Mini-chromosomes as small as 4 Mb are mitotically and structurally stable. This mini-chromosome contains <200 kb of Y alpha satellite, located in two major and one minor array [43].
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Heller R, Brown KE, Burgtorf C, Brown WRA. Minichromosomes derived from the human Y chromosome by telomere-directed chromosome breakage. of special interest Proc Natl Acad Sci USA. 93:1996;7125-7130 This paper reports the latest in a series of elegant experiments to dissect the centromere of the human Y chromosome. Several rounds of telomere-directed chromosome breakage resulted in the generation of small derivatives of the Y that range in size from ≈2.5 to 8 Mb. Mini-chromosomes as small as 4 Mb are mitotically and structurally stable. This mini-chromosome contains <200 kb of Y alpha satellite, located in two major and one minor array [43].
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Proc Natl Acad Sci USA
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Heller, R.1
Brown, K.E.2
Burgtorf, C.3
Brown, W.R.A.4
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25
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0025244949
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Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement
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Wevrick R, Earnshaw WC, Howard-Peebles PN, Willard HF. Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement. Mol Cell Biol. 10:1990;6374-6380.
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Murphy TD, Karpen GH. Localization of centromere function in a Drosophila minichromosome. Cell. 82:1995;599-609.
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Pluta AF, Mackay AM, Ainsztein AM, Goldberg IG, Earnshaw WC. The centromere, hub of chromosomal activities. Science. 270:1995;1591-1594.
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Science
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Pluta, A.F.1
MacKay, A.M.2
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Identification of centromeric antigens in dicentric Robertsonian translocations: CENP-C and CENP-E are necessary components of functional centromeres
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Sullivan BA, Schwartz S. Identification of centromeric antigens in dicentric Robertsonian translocations: CENP-C and CENP-E are necessary components of functional centromeres. Hum Mol Genet. 4:1995;2189-2197.
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Sullivan, B.A.1
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30
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Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres
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of special interest. A long-awaited study describing antibodies to CENP-A and the localization of CENP-A on both normal and abnormal human chromosomes. The study clearly showed the presence of CENP-A at a presumptive neo-centromere and the absence of CENP-A from two inactive centromeres.
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Warburton PE, Cooke CA, Bourassa S, Vafa O, Sullivan BA, Stetten G, Gimelli G, Warburton D, Tyler-Smith C, Sullivan KF, et al. Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres. of special interest Curr Biol. 7:1997;901-904 A long-awaited study describing antibodies to CENP-A and the localization of CENP-A on both normal and abnormal human chromosomes. The study clearly showed the presence of CENP-A at a presumptive neo-centromere and the absence of CENP-A from two inactive centromeres.
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Curr Biol
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Warburton, P.E.1
Cooke, C.A.2
Bourassa, S.3
Vafa, O.4
Sullivan, B.A.5
Stetten, G.6
Gimelli, G.7
Warburton, D.8
Tyler-Smith, C.9
Sullivan, K.F.10
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Human CENP-A contains a histone H3-related histone fold domain that is required for targetting to the centromere
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Sullivan KF, Hechnenberger M, Masri K. Human CENP-A contains a histone H3-related histone fold domain that is required for targetting to the centromere. J Cell Biol. 127:1994;581-592.
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Sullivan, K.F.1
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Masri, K.3
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A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis
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33
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0031049028
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Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites
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of outstanding interest. This very important paper examines the molecular requirements for targeting CENP-A for assembly at centromeres. Targeting requires three distinct regions within CENP-A and further requires that synthesis of CENP-A is uncoupled from histone H3 during S phase. This finding implicates an assembly pathway late in S phase when centromeric DNA typically replicates.
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Shelby RD, Vafa O, Sullivan KF. Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites. of outstanding interest J Cell Biol. 136:1997;501-513 This very important paper examines the molecular requirements for targeting CENP-A for assembly at centromeres. Targeting requires three distinct regions within CENP-A and further requires that synthesis of CENP-A is uncoupled from histone H3 during S phase. This finding implicates an assembly pathway late in S phase when centromeric DNA typically replicates.
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J Cell Biol
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Shelby, R.D.1
Vafa, O.2
Sullivan, K.F.3
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34
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Chromatin containing CENP-A and alpha satellite DNA is a major component of the inner kinetochore plate
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of outstanding interest. of special interest. The latest in a series of papers on the function of CENP-A (see also [30,31,33]), this study addresses the critical issue of what sequences are associated with nucleosomes that contain CENP-A. Kinetochore-associated chromatin was isolated by immunoprecipitation of CENP-A. Cloned DNA fragments from this chromatin revealed that alpha satellite is the major component of functional centromeric DNA.
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Vafa O, Sullivan KF. Chromatin containing CENP-A and alpha satellite DNA is a major component of the inner kinetochore plate. of outstanding interest. of special interest Curr Biol. 7:1997;897-900 The latest in a series of papers on the function of CENP-A (see also [30,31,33]), this study addresses the critical issue of what sequences are associated with nucleosomes that contain CENP-A. Kinetochore-associated chromatin was isolated by immunoprecipitation of CENP-A. Cloned DNA fragments from this chromatin revealed that alpha satellite is the major component of functional centromeric DNA.
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Curr Biol
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Vafa, O.1
Sullivan, K.F.2
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Ekwall K, Olsson T, Turner BM, Cranston G, Allshire RC. Transient inhibition of histone deacetylation alters the structural and functional imprint at fission yeast centromeres. Cell. 91:1997;1021-1032.
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Ekwall, K.1
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Kipling D, Warburton PE. Centromeres, CENP-B and tigger too. Trends Genet. 13:1997;141-145.
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A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B
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Halverson D, Baum M, Stryker J, Carbon J, Clarke L. A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B. J Cell Biol. 136:1997;487-500.
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Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase
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