GPR1 encodes a putative G protein-coupled receptor that associates with the Gpa2p G(α) subunit and functions in a Ras-independent pathway

EMBO Journal

Volumn 17, Issue 7, 1998, Pages 1996-2007

  Xue, Yong ^a   Batlle, Montserrat ^a   Hirsch, Jeanne P ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

G protein; GPA2; GPR1; Ras; Signal transduction; Yeast

Indexed keywords

AMINO ACID; CELL RECEPTOR; CYCLIC AMP; GUANINE NUCLEOTIDE BINDING PROTEIN; NITROGEN; PROTEIN SUBUNIT; RNA;

ALLELE; ARTICLE; CELL GROWTH; CONTROLLED STUDY; CYTOPLASM; GENE MUTATION; HEAT SHOCK; NONHUMAN; ONCOGENE RAS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; YEAST;

AMINO ACID SEQUENCE; AMINO ACIDS; CELL MEMBRANE; CYCLIC AMP; CYTOPLASM; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, RAS; GTP-BINDING PROTEIN ALPHA SUBUNITS; GTP-BINDING PROTEINS; HEAT-SHOCK RESPONSE; HETEROTRIMERIC GTP-BINDING PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; NITROGEN; PHENOTYPE; PROTEIN KINASES; RAS PROTEINS; RECEPTORS, CELL SURFACE; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; RNA, FUNGAL; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION; SPORES, FUNGAL;

EID: 0032055105     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.7.1996     Document Type: Article

References (46)

1. Baldwin,J.M. (1993) The probable arrangement of the helices in G protein-coupled receptors. EMBO J., 12, 1693-1703.
2. Baldwin,J.M. (1994) Structure and function of receptors coupled to G proteins. Curr. Opin. Cell Biol., 6, 180-190.
3. Blumer,K.J. and Thorner,J. (1990) β and γ subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the a subunit but are required for receptor coupling. Proc. Natl Acad. Sci. USA. 87, 4363-4367.
4. Boone,C., Davis,N.G. and Sprague,G.F.,Jr (1993) Mutations that alter the third cytoplasmic loop of the a-factor receptor lead to a constitutive and hypersensitive phenotype. Proc. Natl Acad. Sci. USA, 90, 9921-9925.
5. Broach,J.R. (1991) RAS genes in Saccharomyces cerevisiae: signal transduction in search of a pathway. Trends Genet., 7, 28-33.
6. Cameron,S., Levin,L., Zoller,M. and Wigler,M. (1988) cAMP-independent control of sporulation, glycogen metabolism, and heat shock resistance in S.cerevisiae. Cell, 53, 555-566.
7. Chalfie,M., Tu,Y., Euskirchen,G., Ward,W.W. and Prasher,D.C. (1994) Green fluorescent protein as a marker for gene expression. Science, 263, 802-805.
8. Clark,C.D., Palzkill,T. and Botstein,D. (1994) Systematic mutagenesis of the yeast mating pheromone receptor third intracellular loop. J. Biol. Chem., 269, 8831-8841.
9. Crauwels,M., Donaton,M.C.V., Pemambuco,M.B., Winderickx,J., de Winde,J.H. and Thevelein,J.M. (1997) The Sch9 protein kinase in the yeast Saccharomyces cerevisiae controls cAPK activity and is required for nitrogen activation of the fermentable-growth-medium-induced (FGM) pathway. Microbiology, 143, 2627-2637.
10. Cross,F.R. (1997) 'Marker swap' plasmids: convenient tools for budding yeast molecular genetics. Yeast, 13, 647-653.
11. Cross,F.R. and Tinkelenberg,A.H. (1991) A potential positive feedback loop controlling CLN1 and CLN2 gene expression at the start of the yeast cell cycle. Cell, 65, 875-883.
12. Dietzel,C. and Kurjan,J. (1987) The yeast SCG1 gene: a Gα-like protein implicated in the a- and α-factor response pathway. Cell, 50, 1001-1010.
13. Durnez,P., Pernambuco,M.B., Oris,E., Argüelles,J.-C., Mergelsberg,H. and Thevelein,J.M. (1994) Activation of trehalase during growth induction by nitrogen sources in the yeast Saccharomyces cerevisiae depends on the free catalytic subunits of cAMP-dependent protein kinase, but not on functional Ras proteins. Yeast, 10, 1049-1064.
14. Fields,S. and Song,O. (1989) A novel genetic system to detect protein-protein interactions. Nature, 340, 245-246.
15. sα designed to alter the reactivity of the protein with bacterial toxins. J. Biol. Chem., 264, 21907-21914.
16. Gietz,R.D. and Sugino,A. (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene, 74, 527-534.
17. Guthrie,C. and Fink,G.R. (1991) Guide to Yeast Genetics and Molecular Biology. Academic Press, Inc., San Diego, CA.
18. Hawes,B.E., Luttrell,L.M., Exum,S.T. and Lefkowitz,R.J. (1994) Inhibition of G protein-coupled receptor signaling by expression of cytoplasmic domains of the receptor. J. Biol. Chem., 269, 15776-15785.
19. Hirimburegama,K., Durnez,P., Keleman,J., Oris,E., Vergauwen,R., Mergelsberg,H. and Thevelein,J.M. (1992) Nutrient-induced activation of trehalase in nutrient-starved cells of the yeast Saccharomyces cerevisiae: cAMP is not involved as a second messenger. J. Gen. Microbiol., 138, 2035-2043.
20. Hirsch,J.P. and Cross,F.R. (1993) The pheromone receptors inhibit the pheromone response pathway in Saccharomyces cerevisiae by a process that is independent of their associated Gα protein. Genetics, 135, 943-953.
21. Ito,H., Fukuda,Y., Murata,K. and Kimura,A. (1983) Transformation of intact yeast cells with alkali cations. J. Bacteriol, 153, 163-168.
22. James,P., Halladay,J. and Craig,E.A. (1996) Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics, 144, 1425-1436.
23. Kataoka,T., Broek,D. and Wigler,M. (1985) DNA sequence and characterization of the S.cerevisiae gene encoding adenylate cyclase. Cell 43, 493-505.
24. Kitamura,K. and Shimoda,C. (1991) The Schizosaccharomyces pombe mam2 gene encodes a putative pheromone receptor which has a significant homology with the Saccharomyces cerevisiae Ste2 protein. EMBO J., 10, 3743-3751.
25. König,B., Arendt,A., McDowell,H., Kahlert,M., Hargrave,P.A. and Hofmann,K.P. (1989) Three cytoplasmic loops of rhodopsin interact with transducin. Proc. Natl Acad. Sci. USA, 86, 6878-6882.
26. Kübler,E., Mösch,H.-U., Rupp,S. and Lisanti,M.P (1997) Gpa2p, a G-proiein α-subunit, regulates growth and pseudohyphal development in Saccharomyces cerevisiae via a cAMP-dependent mechanism. J. Biol. Chem., 272, 20321-20323.
27. Lehrach,H., Diamond,D., Wozney,J.M. and Boedtker,H. (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry, 16, 4743-4751.
28. Lorenz,M.C. and Heitman,J. (1997) Yeast pseudohyphal growth is regulated by GPA2, a G protein a homolog. EMBO J., 16, 7008-7018.
29. Matsumoto,K., Uno,I., Oshima,Y. and Ishikawa,T. (1982) Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase. Proc. Natl. Acad. Sci. USA, 79, 2355-2359.
30. Miyajima,I., Nakafuku,M., Nakayama,N., Brenner,C., Miyajima,A., Kaibuchi,K., Arai,K.I., Kaziro,Y. and Matsumoto,K. (1987) GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell, 50, 1011-1019.
31. Morishita.T., Mitsuzawa,H., Nakafuku,M., Nakamura,S., Hattori,S. and Anruku,Y. (1995) Requirement of Saccharomyces cerevisiae Ras for completion of mitosis. Science, 270, 1213-1215.
32. Münch,G., Dees,C., Hekman,M. and Palm,D. (1991) Multisite contacts involved in coupling of the β-adrenergic receptor with the stimulatory guanine-nucleotide-binding regulatory protein. Eur. J. Biochem., 198, 357-364.
33. Nakafuku,M., Itoh,H., Nakamura,S. and Kaziro,Y. (1987) Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the a subunit of mammalian G proteins. Proc. Natl Acad. Sci. USA, 84, 2140-2144.
34. Nakafuku,M. et al. (1988) Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guaninc nucleotide-binding regulatory protein: studies on its structure and possible functions. Proc. Natl Acad. Sci. USA, 85, 1374-1378.
35. Nikawa,J., Cameron,S., Toda,T., Ferguson,K.M. and Wigler,M. (1987) Rigorous feedback control of cAMP levels in Saccharomyces cerevisiae. Genes Dev., 1, 931-937.
36. 2-adrenergic receptor. J. Biol. Chem., 263, 15985-15992.
37. Ohyama,K., Yamano,Y., Chaki,S., Kondo,T. and Inagami,J. (1992) Domains for G-protein coupling in angiotensin II receptor type I: studies by site-directed mutagenesis. Biochem. Biophys. Res. Commun., 189, 677-683.
38. Simon,M.I., Strathmann,M.P. and Gautam,N. (1991) Diversity of G proteins in signal transduction. Science, 252, 802-808.
39. + encodes the putative M-factor receptor. Mol. Cell. Biol., 13, 80-88.
40. Tatchell,K., Chaleff,D.T., DeFeo-Jones,D. and Scolnick,E.M. (1984) Requirement of either of a pair of ras-related genes of Saccharomyces cerevisiae for spore viability. Nature, 309, 523-527.
41. Thevelein,J.M. (1994) Signal transduction in yeast. Yeast, 10, 1753-1790.
42. Toda,T. et al. (1985) In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell, 40, 27-36.
43. Toda,T., Cameron,S., Sass,P., Zoller,M., Scott,J.D., McMullen,B., Hurwitz,M., Krebs,E.G. and Wigler,M. (1987a) Cloning and characteriztion of BCY1, a locus encoding a regulatory subunit of the cAMP dependent protein kinase in yeast. Mol. Cell. Biol., 7, 1371-1377.
44. Toda,T., Cameron,S., Sass,P., Zoller,M. and Wigler,M. (1987b) Three different genes in S.cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell, 50, 277-287.
45. Toda,T., Cameron,S., Sass,P. and Wigler,M. (1988) SCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to. cAMP-dependent protein kinase catalytic subunits. Genes Dev., 2, 517-527.
46. m cyclic AMP phosphodiesterase of Saccharomyces cerevisiae. Mol. Cell. Biol., 8, 505-510.