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Volumn 120, Issue 12, 1998, Pages 2714-2720

Enhancing the proline effect: Pseudo-prolines for tailoring Cis/ trans isomerization

Author keywords

[No Author keywords available]

Indexed keywords

PROLINE;

EID: 0032054074     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja973966s     Document Type: Article
Times cited : (139)

References (46)
  • 10
    • 0013501645 scopus 로고
    • Gierasch, L. M., King, J., Eds.; AAAS Press: Washington, DC
    • Nail, B. T. In Protein Refolding; Gierasch, L. M., King, J., Eds.; AAAS Press: Washington, DC, 1990; pp 198-207.
    • (1990) Protein Refolding , pp. 198-207
    • Nail, B.T.1
  • 19
    • 84920296968 scopus 로고    scopus 로고
    • note
    • Activation of Fmoc-Val-OH with cyanuric fluoride (10 equiv) in the presence of pyridine (1 equiv) in DCM for 2 h under reflux. Filtration of the precipitate and removing of solvent gave Fmoc-Val-F quantilatively.
  • 24
    • 84920296967 scopus 로고    scopus 로고
    • Biosym Technologies Inc., San Diego
    • Biosym Technologies Inc., San Diego.
  • 25
    • 84920296966 scopus 로고    scopus 로고
    • note
    • Integration of Cα protons affords the same cis content. Due to signal overlap in some cases, the NH-pNA was chosen for all compounds to determine their cis/trans ratios.
  • 27
    • 84920296965 scopus 로고    scopus 로고
    • note
    • -1.
  • 29
    • 84920296964 scopus 로고    scopus 로고
    • note
    • 1H NMR was observed.
  • 38
    • 84920296963 scopus 로고    scopus 로고
    • note
    • The cis/trans ratios of all compounds were determined by the integration of the 2-C protons which for each of the cis and the trans form give separate singlet-signals at around 6.1 and 6.2 ppm, respectively.
  • 39
    • 84920296962 scopus 로고    scopus 로고
    • note
    • 1H NMR spectrum in DMSO shows one predominant conformation. For certain protons such as the NH-pNA proton, traces of another conformation can be detected. However, due to a relative intensity of less than 3% with respect to the major conformation, an unambiguous assignment is impossible. It was yet detected by the CT coupled assay that very few percent of the trans form exist depending on temperature and solvent.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.