Ferric citrate uptake by cultured rat hepatocytes is inhibited in the presence of transferrin

European Journal of Biochemistry

Volumn 253, Issue 1, 1998, Pages 139-145

  Graham, Ross M ^a   Morgan, Evan H ^a   Baker, Erica ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Ferric citrate; Iron transport; Non transferrin bound iron; Rat hepatocytes; Transferrin

Indexed keywords

FERRIC CITRATE; TRANSFERRIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; DRUG UPTAKE; IRON STORAGE; IRON TRANSPORT; ISOTOPE LABELING; LIVER; LIVER CELL; LIVER CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; RAT;

ANIMALIA;

EID: 0032053475     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2530139.x     Document Type: Article

References (34)

1. Hershko, C. & Rachmilewitz, E. A. (1975) Non-transferrin plasma iron in patients with transfusional iron overload, in Proteins of iron storage and transport in biochemistry and medicine (Crichton, R. R., ed.) pp. 427 - 432. North Holland Publishing Company, Amsterdam.
2. Hershko, C., Graham, G., Bates, G. W. & Rachmilewitz, E. A. (1978) Non-specific serum iron in thalassemia: an abnormal serum iron fraction of potential toxicity, Br. J. Haematol. 40, 235 - 263.
3. Graham, G., Bates, G. W., Rachmilewitz, E. A. & Hershko, C. (1979) Nonspecific serum iron in thalassemia: quantitation and chemical reactivity, Am. J. Hematol. 6, 207 - 217.
4. Fargion, S., Cappellini, M. D., Sampietro, M. & Fiorelli, G. (1981) Non-specific iron in patients with beta-thalassaemia trait and chronic active hepatitis, Scand. J. Haematol. 26, 161 - 167.
5. Fawwaz, R. A., Winchell, H. S., Pollycove, M. & Sargent, T. (1967) Hepatic iron deposition in humans. I. First-pass hepatic deposition of intestinally absorbed iron in patients with low plasma latent iron-binding capacity. Blood 30, 417 - 424.
6. Batey, R. G., Fong, P. L. C., Shamir, S. & Sherlock, S. (1980) A non-transferrin-bound serum iron in idiopathic hemochromatosis. Dig. Dis. Sci. 25, 340 - 346.
7. Gutteridge, J. M. C., Rowley, D. A., Griffiths, E. & Halliwell, B. (1985) Low-molecular-weight iron complexes and oxygen radical reactions in idiopathic haemochromatosis, Clin. Sci. 68, 463 - 467.
8. Grootveld, M., Bell, J. D., Halliwell, B., Aruoma, O. I., Bomford, A. & Sadler, P. J. (1989) Non-transferrin-bound iron in plasma or serum from patients with idiopathic hemochromatosis. Characterization by high performance liquid chromatography and nuclear magnetic resonance spectroscopy. J. Biol. Chem. 264, 4417 - 4422.
9. Carmine, T. C., Evans, P., Bruchelt, G., Evans, R., Handgretinger, R., Niethammer, D. & Halliwell, B. (1995) Presence of iron catalytic for free radical reactions in patients undergoing chemotherapy: implications for therapeutic management, Cancer Lett. 94, 219 - 226.
10. Sarkar, B. (1970) State of iron(III) in normal human serum: low molecular weight and protein ligands besides transferrin. Can. J. Biochem. 48, 1339 - 1350.
11. White, G. P. & Jacobs, A. (1978) Iron uptake by Chang cells from transferrin, nitriloacetate and citrate complexes: the effects of iron-loading and chelation with desferrioxamine, Biochim. Biophys. Acta 543, 217 - 225.
12. Morgan, E. H. (1988) Membrane transport of non-transferrin-bound iron by reticulocytes, Biochim. Biophys. Acta 943, 428 - 439.
13. Scheiber, B. & Goldenberg, H. (1996) Uptake of iron by isolated rat hepatocytes from a hydrophilic impermeant ferric chelate, Fe(III)-DTPA, Arch. Biochem. Biophys. 659, 185 - 192.
14. Wheby, M. S. & Jones, L. G. (1963) Role of transferrin in iron absorption, J. Clin. Invest. 42, 1007 - 1016.
15. Wheby, M. S. & Umpierre, G. (1964) Effect of transferrin saturation on iron absorption in man, N. Engl. J. Med. 271, 1391 - 1395.
16. Brissot, P., Wright, T. L., Ma, W.-L. & Weisiger, R. A. (1985) Efficient clearance of non-transferrin-bound iron by rat liver: implications for hepatic iron loading in iron overload states, J. Clin. Invest. 76, 1463 - 1470.
17. Morgan, E. H., Heubers, H. & Finch, C. A. (1978) Differences between the binding sites for iron binding and release in human and rat transferrin. Blood 52, 1219 - 1228.
18. Hemmaplardh, D. & Morgan, E. H. (1976) A comparative study of chemically and enzymatically radioiodinated transferrin, Int. J. Appl. Radiat. Isotop. 27, 89 - 92.
19. Page, M. A., Baker, E. & Morgan, E. H. (1984) Transferrin and iron uptake by rat hepatocytes in culture, Am. J. Physiol. 246, G26 - G33.
20. Graham, R. M., Morgan, E. H. & Baker, E. (1998) Characterisation of citrate and iron uptake by cultured rat hepatocytes, J. Hepatol., in the press.
21. Labarca, C. & Paigen, K. (1980) A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102, 344 - 352.
22. Kennedy, M. C., Mende-Mueller, L., Blondin, G. A. & Beinert, H. (1992) Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein. Proc. Natl Acad. Sci. USA 89, 11 730 - 11 734.
23. Trinder, D. & Morgan, E. H. (1997) Inhibition of uptake of transferrin-bound iron by human hepatoma cells by non-transferrin bound iron, Hepatol. 26, in the press.
24. Bates, G. W., Billups, C. & Saltman, P. (1967) The kinetics and mechanism of iron(III) exchange between chelates and transferrin. I. The complexes of citrate and nitrilotriacetic acid. J. Biol. Chem. 242, 2810 - 2815.
25. Morgan, E. H. & Baker, E. (1986) Iron uptake and metabolism by hepatocytes, Fed. Proc. 45, 2801 - 2816.
26. Bacon, B. R. & Tavill, A. S. (1984) Role of the liver in normal iron metabolism, Semin. Liver Dis. 4, 181 - 192.
27. Nilsen, T. (1991) Effects of calcium on hepatocyte iron uptake from transferrin, iron-pyrophosphate and iron-ascorbate, Biochim. Biophys. Acta 1095, 39 - 45.
28. Brittenham, G. M. (1994) The red cell cycle, in Iron metabolism in health and disease (Brock, J. H., Halliday, J. W., Pippard, M. J. & Powell, L. W., eds) pp. 31 - 62, W. B. Saunders Company, Ltd. London.
29. Morgan, E. H. (1996) Iron metabolism and transport, in Hepatology. A textbook of liver disease, 3rd edn (Zakim, D. & Boyer, T. D., eds) pp. 526 - 554, W. B. Saunders Company, Sydney.
30. Cole, E. S. & Glass, J. (1983) Transferrin binding and iron uptake in mouse hepatocytes, Biochim. Biophys. Acta 762, 102 - 110.
31. Thorstensen, K. & Romslo, I. (1984) Uptake of iron from transferrin by isolated hepatocytes, Biochim. Biophys. Acta 804, 200 - 208.
32. Trinder, D., Morgan, E. & Baker, E. (1986) The mechanisms of iron uptake by fetal rat hepatocytes in culture, Hepatology 6, 852 - 858.
33. Trinder, D., Morgan, E. H. & Baker, E. (1988) The effects of an antibody to the rat transferrin receptor and of rat serum albumin on the uptake of diferric transferrin by the rat hepatocyte. Biochim. Biophys. Acta 943, 440 - 446.
34. Trinder, D., Zak, O. & Aisen, P. (1996) Transferrin receptor-independent uptake of diferric transferrin by human hepatoma cells with antisense inhibition of receptor expression. Hepatology 23, 1512 - 1520.