Efficient production of heat-labile enterotoxin mutant proteins by overexpression of dsbA in a degP-deficient Escherichia coli strain

Archives of Microbiology

Volumn 167, Issue 5, 1997, Pages 280-283

  Wülfing, Christoph ^a,b   Rappuoli, Rino ^a  

^a CHIRON VACCINES   (Italy)

^b STANFORD UNIVERSITY   (United States)

Author keywords

Bacterial toxin; Chaperones; Cholera; CT; Disulfide bridge; DsbA; Enterotoxigenic E. coli; Escherichia coli enterotoxin; LT; Protein folding

Indexed keywords

DISULFIDE; ESCHERICHIA COLI ENTEROTOXIN; PROTEINASE;

ARTICLE; ENZYME DEFICIENCY; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING;

BACTERIAL TOXINS; BASE SEQUENCE; ENTEROTOXINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HEAT-SHOCK PROTEINS; ISOMERASES; OLIGODEOXYRIBONUCLEOTIDES; PERIPLASMIC PROTEINS; PLASMIDS; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN DISULFIDE-ISOMERASE; PROTEIN FOLDING; SERINE ENDOPEPTIDASES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030897393     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002030050444     Document Type: Article

References (26)

1. Bardwell JC (1994) Building bridges: disulphide bond formation in the cell. Mol Microbiol 14:199-205
2. Brent R, Ptashne M (1981) Mechanisms of action of the lexA. Proc Natl Acad Sci USA 78:4204-4208
3. Carter P, Bedouelle H, Winter G (1985) Improved oligonucleotide site-directed mutagenesis using M13 vectors. Nucleic Acids Res 13:4431-4443
4. Cavard D (1995) Role of DegP protease on levels of various forms of colicin A lysis protein. FEMS Microbiol Lett 125:173-178
5. Domenighini M, Magagnoli C, Pizza M, Rappuoli R (1994) Common features of the NAD-binding and catalytic site of ADP-ribosylating toxins. Mol Microbiol 14:41-50
6. Erikson JW, Gross CA (1989) Identification of the SE subunit of Escherichia coli RNA polymerase: a second alternate s factor involved in high-temperature gene expression. Genes Dev 3: 1462-1471
7. Hardy SJ, Holmgren J, Johansson S, Sanchez J, Hirst TR (1988) Coordinated assembly of multisubunit proteins: oligomerization of bacterial enterotoxins in vivo and in vitro. Proc Natl Acad Sci USA 85:7109-7113
8. Hirst TR (1994) Biogenesis of cholera toxin and related oligomeric enterotoxins. In: Moss J, Vaughan M, Iglewski B, Tu AT (eds) Bacterial toxins and virulence factors in disease handbook of natural toxins, vol 8, pp 123-184
9. Knappik A, Krebber C, Plückthun A (1993) The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in Escherichia coli. Biotechnology 11: 77-83
10. Kucharczyk K, Laskowska E, Taylor A (1991) Response of Escherichia coli cell membranes to induction of c1857 prophage by heat shock. Mol Microbiol 5:2935-2945
11. Maurizi MR (1992) Proteases and protein degradation in Escherichia coli. Experientia 48:178-201
12. Misra R, Peterson A, Ferenci T, Silhavy TJ (1991) A genetic approach for analyzing the pathway of LamB assembly into the outer membrane of Escherichia coli. J Biol Chem 266: 13592-13597
13. Pizza M, Domenighini M, Hol W, Giannelli V, Fontana MR, Giuliani MM, Magagnoli C, Peppoloni S, Manetti R, Rappuoli R (1994) Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis. Mol Microbiol 14:51-60
14. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
15. Sato K, Sato MH, Yamaguchi A, Yoshida M (1994) Tetracycline/H+ antiporter was degraded rapidly in Excherichia coli cells when truncated at last transmembrane helix and this degradation was protected by overproduced GroEL/ES. Biochem Biophys Res Commun 202:258-264
16. Skerra A (1989) Funktionelle Expression Antigen bindender Immunoglobulinfragmente in Escherichia coli. PhD Thesis. University of Munich, Germany
17. Staley JP, Kim PS (1992) Complete folding of bovine pancreatic trypsin inhibitor with only a single disulfide bond. Proc Natl Acad Sci USA 89:1519-1523
18. Strauch KL, Beckwith J (1988) An Escherichia coli mutation preventine degradation of abnormal periplasmic proteins. Proc Natl Acad Sci USA 85:1576-1580
19. Van Mierlo CP, Darby NJ, Neuhaus D, Creighton TE (1991) (14-38, 30-51) double-disulphide intermediate in folding of bovine pancreatic trypsin inhibitor: a two-dimensional 1H nuclear magnetic resonance study. J Mol Biol 222:353-371
20. Wawrzynow A, Wojtkowiak D, Marszalek J, Banecki B, Jonsen M, Graves B, Georgopoulos C, Zylicz M (1995) The ClpX heat-shock protein of Escherichia coli, the ATP-dependent substrate specificity component of the ClpP-ClpX protease, is a novel molecular chaperone. EMBO J 14:1867-1877
21. Weissman JS, Kim PS (1991) Reexamination of the folding of BPTI: predominance of native intermediates. Science 253: 1386-1393
22. Wülfing C, Plückthun A (1994a) Protein folding in the periplasm of Escherichia coli. Mol Microbiol 12:685-692
23. Wülfing C, Plückthun A (1994b) Correctly folded T-cell receptor fragments in the periplasm of Excherichia coli. Influence of folding catalysts. J Mol Biol 242:655-669
24. Wunderlich M, Glockshuber R (1993) In vivo control of redox potential during protein folding catalyzed by bacterial protein disulfide-isomerase (DsbA). J Biol Chem 268:24547-24550
25. Yu J, Webb H, Hirst TR (1992) A homologue of the Excherichia coli DsbA protein involved in disulphide bond formation is required for enterotoxin biogenesis in Vibrio cholerac. Mol Microbiol 6:1949-1958
26. Zdanovsky AG, Kulaeva OI, Yankovsky NK (1992). Construction and expression of diphtheria toxin-encoding gene derivatives in Escherichia coli. Gene 116:81-86