Characterization and cDNA cloning of a hemoprotein in the salivary glands of the blood-sucking insect, Rhodnius prolixus

Insect Biochemistry and Molecular Biology

Volumn 28, Issue 3, 1998, Pages 191-200

  Sun, Jianxin ^a   Yuda, Masao ^a   Miura, Ken ^a   Chinzei, Yasuo ^a  

^a MIE UNIVERSITY   (Japan)

Author keywords

Anticoagulant; Hemoprotein; NO carrier; Rhodnius prolixus; Salivary glands

Indexed keywords

COMPLEMENTARY DNA; HEMOPROTEIN; INSECT PROTEIN; NITRIC OXIDE; NITROPHORIN; PRIMER DNA; SALIVA PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; GENETICS; GROWTH, DEVELOPMENT AND AGING; INSECT; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; RHODNIUS; SALIVARY GLAND; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; HEMEPROTEINS; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; NITRIC OXIDE; RHODNIUS; SALIVARY PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

ANIMALIA; INSECTA; RHODNIUS; RHODNIUS PROLIXUS;

EID: 0032029865     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(97)00115-X     Document Type: Article

References (17)

1. Champagne D. E., Nussenzveig R. H., Ribeiro J. M. C. Purification, partial characterization, and cloning of nitric oxide-carrying heme proteins (Nitrophorins) from salivary glands of the blood-sucking insect Rhodnius prolixus. J. Biol. Chem. 270:1995;8691-8695.
2. Chinzei Y., Azumi C., Miura K., Matsuoka H., Ando K. Changes in hemolymph proteins and their synthetic activities during development and engorgement, and after juvenile hormone treatment in the kissing bug Rhodnius prolixus. J. Insect Physiol. 40:1994;491-499.
3. Hellmann K., Hawkins R. I. Prolixin-S and Prolixin-G; two anticoagulants from Rhodnius prolixus stal. Nature. 207:1965;1265-1267.
4. Ribeiro J. M. C. Role of saliva in blood-feeding by arthropods. Ann. Rev. Entomol. 32:1987;463-478.
5. Ribeiro J. M. C. Blood-feeding arthropods: Live syringes or invertebrate pharmacologists? Infectious Agents and Disease. 4:1995;143-152.
6. Ribeiro J. M. C., Garcia E. S. The salivary and crop apyrase activity of Rhodnius prolixus. J. Insect Physiol. 26:1980;303-307.
7. Ribeiro J. M. C., Sarkis J. J. F. Anti-thromboxane activity in Rhodnius prolixus salivary secretion. J. Insect Physiol. 28:1982;655-660.
8. Ribeiro J. M. C., Walker F. A. High affinity histamine-binding and antihistaminic activity of the salivary nitric oxide-carrying heme protein (Nitrophorin) of Rhodnius prolixus. J. Exp. Med. 180:1994;2251-2257.
9. Ribeiro J. M. C., Marinotti O., Gonzales R. A salivary vasodilator in the blood-sucking bug Rhodnius prolixus. Br. J. Pharmacol. 101:1990;932-936.
10. Ribeiro J. M. C., Schneider M., Guimarases J. A. Purification and characterization of prolixin S (nitrophorin 2), the salivary anticoagulant of the blood-sucking bug Rhodnius prolixus. Biochem. J. 308:1995;243-249.
11. Ribeiro J. M. C., Hazzard J. M. H., Nussenzveig R. H., Champagne D. E., Walker F. A. Reversible binding of nitric oxide by a salivary heme protein from a bloodsucking insect. Science. 260:1993;539-541.
12. Sambrook, J., Fritsch, E. F., Maniatis, T. 1989. Molecular cloning: a laboratory manual. (2nd ed.). Cold Spring Harbor Laboratory, New York.
13. Sarkis J. J. F., Guimaraes J. A., Ribeiro J. M. C. Salivary apyrase of Rhodnius prolixus: kinetics and purification. Biochem. J. 233:1986;885-891.
14. Stark K. R., James A. A. Anticoagulants in vector arthropods. Parasitol. Today. 12:1996;430-437.
15. Sun J., Yamaguchi M., Yuda M., Miura K., Takeya H., Hirai M.et al. Purification, characterization and cDNA cloning of a novel anticoagulant of the intrinsic pathway, (Prolixin-S), from salivary glands of the blood sucking bug Rhodnius prolixus. Thromb. and Haemost. 75:1996;573-577.
16. Yuda M., Sun J., Yamaguchi M., Ando K., Chinzei Y. Identification and activities of anticoagulants in the salivary glands of the blood sucking kissing bug Rhodnius prolixus. Med. Entomol. Zool. 47:1996;263-272.
17. Yuda M., Higuchi K., Sun J., Kureishi Y., Ito M., Chinzei Y. Expression, reconstitution and characterization of prolixin-S as a vasodilator: A salivary gland nitric oxide-binding hemoprotein of Rhodnius prolixus. Eur. J. Biochem. 249:1997;337-342.