Expression, reconstitution and characterization of prolixin-S as a vasodilator. A salivary gland nitric-oxide-binding hemoprotein of Rhodnius prolixus

European Journal of Biochemistry

Volumn 249, Issue 1, 1997, Pages 337-342

  Yuda, Masao ^a   Higuchi, Kuniko ^a   Sun, Jianxin ^a   Kureishi, Yasuko ^a   Ito, Masaaki ^a   Chinzei, Yasuo ^a  

^a MIE UNIVERSITY   (Japan)

Author keywords

Hemoprotein; Nitric oxide; Salivary gland

Indexed keywords

ANTICOAGULANT AGENT; HEMOPROTEIN; PROLIXIN S; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ABSORPTION SPECTROSCOPY; ANIMAL TISSUE; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; HEMIPTERA; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SALIVARY GLAND;

ANIMALIA; HEMIPTERA; INSECTA; RHODNIUS PROLIXUS; UNIDENTIFIED BACULOVIRUS;

EID: 0030879304     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00337.x     Document Type: Article

References (13)

1. Sun, J., Yamaguchi, M., Yuda, M., Miura, K., Takeya, H., Hirai, M., Matsuoka, H., Ando, K., Watanabe, T., Suzuki, K. & Chinzei, Y. (1996) Purification, characterization and cDNA cloning of a novel anticoagulant of the intrinsic pathway (prolixin-S), from salivary glands of the blood sucking bug, Rhodnius prolixus, Thromb. Haemostasis 75, 573-577.
2. Ribeiro, J. M. C. & Garcia, E. S. (1981) Platelet antiaggregating activity in the salivary secretion of the blood sucking bug Rhodnius prolixus, Experientia (Basel) 37, 384-386.
3. Ribeiro, J. M. C. (1982) The antiserotonin and antihistamine activities of salivary secretion of Rhodnius prolixus, J. Insect Physiol. 28, 69-75.
4. Ribeiro, J. M. C. (1993) Reversible binding of nitric oxide by a salivary heme protein from a blood sucking insect, Science 260, 539-541.
5. Yuda, M., Makoto, H., Miura, K., Ando, K. & Chinzei, Y. (1996) cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect, Rhodnius prolixus, Eur. J. Biochem. 242, 807-812.
6. Max, D. S. & Gale, E. S. (1987) A manual of methods for baculovirus vectors and insect cell culture procedures, pp. 29-30, Texas Agricultural Experiment Station, College Station, Texas.
7. Laemmli, U. K. (1970) Cleavage of structual proteins during assembly of the head of bacteriophage-T, Nature 227, 680-685.
8. Ascoli, F., Fanelli, M. R. R. & Antonini, E. (1981) Preparation and properties of apohemoglobin and reconstituted hemoglobins, Methods Enzymol. 76, 72-87.
9. Shibata, Y., Matsui, K., Kajiwara, T. & Hatanaka, A. (1995) Fatty acid hydroperoxide lyase is a heme protein, Biochem. Biophys. Res. Commun. 207, 438-443.
10. Champagne, D. E., Nussenzweig, R. H. & Ribeiro, J. M. C. (1995) Purification, partial characterization, and cloning of nitric oxide-carrying heme proteins (nitrophorins) from salivary glands of the blood-sucking insect Rhodnius prolixus, J. Biol. Chem. 270, 8691-8695.
11. Varadarajan, R., Szabo, A. & Boxer, S. G. (1985) Cloning, expression in Escherichia coli,and reconstitution of human myoglobin, Proc. Natl Acad. Sci. USA 82, 5681-5684.
12. Di Iorio, E. (1981) Preparation of derivatives of ferrous and ferric hemoglobin, Methods Enzymol. 76, 57-72.
13. Sharma, V. S., Traylor, T. G., Gardiner, R. & Mizukami, H. (1987) Reaction of nitric oxide with heme proteins and model compounds of hemoglobin, Biochemistry 26, 3837-3843.