The human trifunctional enzyme of de novo purine biosynthesis: Heterologous expression, purification, and preliminary characterization

Protein Expression and Purification

Volumn 12, Issue 1, 1998, Pages 17-24

  Poch, Mark T ^a   Qin, Wen ^a   Caperelli, Carol A ^a  

^a UNIVERSITY OF CINCINNATI MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

10 FORMYL 5,8 DIDEAZAFOLATE; AMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE; BIOSYNTHESIS; COMPLEMENTARY DNA; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; FOLIC ACID; GLYCINAMIDE RIBONUCLEOTIDE TRANS FORMYLASE; KINETICS; PHOSPHORIBOSYLAMINE GLYCINE LIGASE; PROTEIN EXPRESSION; PROTEIN PRODUCT; PROTEIN PURIFICATION; PURINE;

EID: 0032004880     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0799     Document Type: Article

References (33)

1. Schild D., Brake A. J., Kiefer M. C., Young D., Barr P. J. Cloning of three human multifunctionalde novo. Proc. Natl. Acad. Sci. USA. 87:1990;2916-2920.
2. Aimi J., Qiu H., Williams J., Zalkin H., Dixon J. E. De novoE. coli. Nucleic Acids Res. 18:1990;6665-6672.
3. Kan J. L. C., Jannatipour M., Taylor S. M., Moran R. G. Mouse cDNAs encoding a trifunctional protein ofde novo. Gene. 137:1993;195-202.
4. Smith J. M., Daum H. A. III. Identification and nucleotide sequence of a gene encoding 5′-phosphoribosylglycinamide transformylase inEscherichia coli. J. Biol. Chem. 262:1987;10565-10569.
5. Inglese J., Johnson D. L., Shiau A., Smith J. M., Benkovic S. J. Subcloning, characterization, and affinity labeling ofEscherichia coli. Biochemistry. 29:1990;1436-1443.
6. White J. H., Lusnak K., Fogel S. Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate. Nature (London). 315:1985;350-352.
7. Ebbole D. J., Zalkin H. Cloning and characterization of a 12-gene cluster fromBacillus subtilisde novo. J. Biol. Chem. 262:1987;8274-8287.
8. Beardsley G. P., Taylor E. C., Moroson B. A., Moran R. G. A new folate antimetabolite, 5,10-dideaza-5,6,7,8-tetrahydrofolate, is a potent inhibitor ofde novo. J. Biol. Chem. 264:1989;328-333.
9. Moran R. G., Baldwin S. W., Taylor E. C., Shih C. The 6S- and 6R-diastereomers of 5,10-dideaza-5,6,7,8-tetrahydrofolate are equiactive inhibitors ofde novo. J. Biol. Chem. 264:1989;21047-21051.
10. Kan C.-C., Gehring M. R., Nodes B. R., Janson C. A., Almassy R. J., Hostomska Z. Heterologous expression and purification of active human phosphoribosylglycinamide formyltransferase as a single domain. J. Protein Chem. 11:1992;467-473.
11. Caperelli C. A., Giroux E. L. The human glycinamide ribonucleotide transformylase domain: Purification, characterization, and kinetic mechanism. Arch. Biochem. Biophys. 341:1997;98-103.
12. Studier F. W., Moffatt B. A. Use of bacteriophage T7 RNA polymerase to direct high-level expression of cloned genes. J. Mol. Biol. 189:1986;113-130.
13. Chettur G., Benkovic S. J. A new chemical synthesis of 2-amino-(N-D-ribofuranosyl)acetamide-5′-phosphate. Carbohydr. Res. 56:1977;75-86.
14. Schendel F. J., Stubbe J. Substrate specificity of formylglycinamidine ribonucleotide synthetase. Biochemistry. 25:1986;2256-2264.
15. Ames B. N. Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol. 8:1966;115-118.
16. Caperelli C. A., Conigliaro J. Synthesis of 10-acetyl-5,8-dideazafolic acid: A potent inhibitor of glycinamide ribonucleotide transformylase. J. Med. Chem. 29:1986;2117-2119.
17. Smith G. K., Mueller W. T., Benkovic P. A., Benkovic S. J. On the cofactor specificity of glycinamide ribonucleotide transformylase and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase. Biochemistry. 20:1981;1241-1245.
18. Caperelli C. A. Mammalian glycinamide ribonucleotide transformylase: Purification and some properties. Biochemistry. 24:1985;1316-1320.
19. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London). 227:1970;680-685.
21. Caperelli C. A. Mammalian glycinamide ribonucleotide transformylase: Kinetic mechanism and associatedde novo. J. Biol. Chem. 264:1989;5053-5057.
22. Cleland W. W. Statistical analysis of enzyme kinetic data. Methods Enzymol. 63:1979;103-138.
23. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
24. Gouy M., Gautier C. Codon usage in bacteria: Correlation with gene expressivity. Nucleic Acids Res. 10:1982;7055-7074.
25. Young M., Sammons R. D., Mueller W. T., Benkovic S. J. An antibody probe to determine the native species of glycinamide ribonucleotide transformylase in chicken liver. Biochemistry. 23:1984;3979-3986.
26. Arnold F. H. Metal-affinity separations: A new dimension in protein processing. Bio/Technology. 12:1991;151-156.
27. Fromm H. J. Use of competitive inhibitors to study substrate binding order. Methods Enzymol. 63:1979;467-486.
28. Dalziel K. Initial steady-state velocities in the evaluation of enzyme-coenzyme-substrate reaction mechanisms. Acta Chem. Scand. 11:1957;1706-1723.
29. Stone R. L., Zalkin H., Dixon J. E. Expression, purification, and kinetic characterization of recombinant human adenylosuccinate lyase. J. Biol. Chem. 268:1993;19710-19716.
30. Steczko J., Donoho G. P., Dixon J. E., Sugimoto T., Axelrod B. Effect of ethanol and low-temperature culture on expression of soybean lipoxygenase L-1 inEscherichia coli. Protein Expression Purif. 2:1991;221-227.
31. Daubner S. C., Benkovic S. J. Characterization of mammalian phosphoribosylglycinamide formyltransferase from transformed cells. Cancer Res. 45:1985;4990-4997.
32. Baldwin S. W., Tse A., Gossett L. S., Taylor E. C., Rosowsky A., Shih C., Moran R. G. Structural features of 5,10-dideaza-5,6,7,8-tetrahydrofolate that determine inhibition of mammalian glycinamide ribonucleotide formyltransferase. Biochemistry. 30:1991;1997-2006.
33. Antle V. D., Liu D., McKellar B. R., Caperelli C. A., Hua M., Vince R. Substrate specificity of glycinamide ribonucleotide transformylase from chicken liver. J. Biol. Chem. 271:1996;6045-6049.