The detection of Glomus spp. (arbuscular mycorrhizal fungi) forming mycorrhizas in three plants, at different stages of seedling development, using mycorrhiza-specific isozymes

New Phytologist

Volumn 138, Issue 2, 1998, Pages 225-239

  Tisserant, B ^a   Brenac, V ^a   Requena, N ^b   Jeffries, P ^a   Dodd, J C ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

^b CSIC   (Spain)

Author keywords

Alkaline phosphatase; Anthyllis cytisoides L.; Desertified ecosystem; Mycorrhiza specific isozymes

Indexed keywords

ARBUSCULAR MYCORRHIZAE; FUNGUS; MYCORRHIZA; SEEDLING DEVELOPMENT;

SPAIN;

ALLIUM PORRUM; ANTHYLLIS CYTISOIDES; GLOMUS; GLOMUS MICROAGGREGATUM; THYMUS VULGARIS;

EID: 0031978977     PISSN: 0028646X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1469-8137.1998.00112.x     Document Type: Article

References (35)

1. Abbott LK. 1982. Comparative anatomy of vesicular-arbuscular mycorrhizas formed on subterranean clover. Australian Journal of Botany 30: 485-499.
2. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
3. Clapp JP, Young JPW, Merryweather JW, Fitter AH. 1995. Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community. New Phytologist 130: 259-265.
4. Dodd JC, Arias I, Koomen I, Hayman DS. 1990a. The management of vesicular-arbuscular mycorrhizal populations in acid-infertile soils of a savanna ecosystem I. The effect of pre-cropping and VAMF inoculation on plant growth and nutrition in the field. Plant and Soil 122: 229-240.
5. Dodd JC, Arias I, Koomen I, Hayman DS. 1990b. The management of vesicular-arbuscular mycorrhizal populations in acid-infertile soils of a savanna ecosystem II. The effects of inoculation and pre-crops on the native VAMF spore populations. Plant and Soil 122, 241-247.
6. Dodd JC, Rosendahl S, Giovannetti M, Broome A, Lanfranco L, Walker C. 1996. Inter- and intraspecific variation within the morphologically-similar arbuscular mycorrhizal fungi Glomus mosseae and Glomus coronatum. New Phytologist 133: 113-122.
7. Gazey C, Abbott LK, Robson AD. 1992. The rate of development of mycorrhizas affects the onset of sporulation and production of external hyphae by two species of Acaulospora. Mycological Research 96: 643-650.
8. Geeson NA, Thornes JB. (eds) 1996. Medalus II-Mediterranean Desertification and Land Use-Executive Summary Phase II. EU Environment and Climate Programme, 1-30.
9. Gianinazzi-Pearson V, Gianinazzi S. 1978. Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. II. Soluble alkaline phosphatase specific to mycorrhizal infection in onion roots. Physiological Plant Pathology 12: 45-53.
10. Giovannetti M, Gianinazzi-Pearson V. 1994. Biodiversity in arbuscular mycorrhizal fungi. Mycological Research 98: 703-715.
11. Hamel C, Fyles H, Smith DL. 1990. Measurement of development of endomycorrhizal mycelium using three different vital stains. New Phytologist 115: 297-302.
12. Hepper CM, Sen R, Maskall CS. 1986. Identification of vesicular-arbuscular mycorrhizal fungi in roots of leek (Allium porrum L.) and maize (Zea mays L.) on the basis of enzyme mobility during polyacrylamide gel electrophoresis. New Phytologist 102: 529-539.
13. Hepper CM, Azcón-Aguilar C, Rosendahl S, Sen R. 1988a. Competition between three species of Glomus used as spatially separated introduced and indigenous mycorrhizal inoculation for leek (Allium porrum L.). New Phytologist 110: 207-215.
14. Hepper CM, Sen R, Azcón-Aguilar C, Grace C. 1988b. Variation in certain isozymes amongst different geographical isolates of the vesicular-arbuscular mycorrhizal fungi Glomus darum, Glomus monosporum and Glomus mosseae. Soil Biology and Biochemistry 20: 51-59.
15. Kjøller R, Rosendahl S. 1996. Arbuscular mycorrhizal fungi in roots from a Danish pea field determined by polyacrylamide gel electrophoresis of specific fungal enzymes. Abstract of oral presentation at 1st International Conference on Mycorrhizae, San Francisco, CA, USA. July 31-August 3 1996.
16. Kough JL, Gianinazzi-Pearson V, Gianinazzi S. 1987. Depressed metabolic activity of vesicular-arbuscular fungi after fungicide application. New Phytologist 106: 707-715.
17. Mejía D, Cano C, Mayer J. 1996. Identificación electroforética de hongos formadores de micorrizas arbusculares: Evaluación de patrones isoenzimaticos. In: Eduardo Guerrero Forero, ed. Micorrizas-Recurso Biológico del Suelo. Fondo Fen, Bogota, Colombia, 145-180.
18. Phillips JM, Hayman, DS. 1970. Improved procedure for clearing roots and staining parasitic and vesicular-arbuscular fungi for rapid assessment of infection. Transactions of the British Mycological Society 55: 158-161.
19. Ravolanirina F, Gianinazzi-Pearson V, Gianinazzi S. 1992. Endomycorrhization VA des vitro plant de vigne: approche immunologique pour la detection des champignons impliques. In: Proceedings Interactions Plantes Microorganismes (anonymous editor). Dakar, Senegal: IFS and ORSTROM, 186-198.
20. Rivillas C, Dodd JC. 1996. The effects of arbuscular mycorrhizal fungi on two different coffee varieties from Colombia and their biochemical detection in roots. In: Mycorrhizas in Integrated Systems from Genes to Plant Development. Proceedings of 4th European Symposium on Mycorrhizas, Granada, Spain 11-14 July 1994. EC Brussels, 47-50.
21. Rosendahl S. 1992. Influence of three vesicular-arbuscular mycorrhizal fungi (Glomales) on the activity of metabolic enzymes of the host plant. Plant and Soil 144: 219-226.
22. Rosendahl S, Dodd JC, Walker C. 1994. Taxonomy and phylogeny of the Glomales. In: Gianinazzi S, Schüepp H, eds. Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystem. Basel, Switzerland : Birkhäuser Verlag, 1-12.
23. Rosendahl S, Sen R, Hepper CM, Azcón-Aguilar C. 1989. Quantification of three vesicular-arbuscular mycorrhizal fungi (Glomus spp.) in roots of leek (Allium porrum) on the basis of activity of diagnostic enzymes after polyacrylamide gel electrophoresis. Soil Biology and Biochemistry 4: 519-522.
24. Sanders IR, Alt M, Groppe K, Boiler T, Wiemken A. 1995. Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: application to studies on the genetic diversity of arbuscular mycorrhizal communities. New Phytologist 130: 419-427.
25. Sen R. 1990. Isozymic identification of individual ectomycorrhizas synthesized between Scots pine (Pinus sylvestris L.) and isolates of two species of Suillus. New Phytologist 114: 617-626.
26. Sen R, Hepper CM. 1986. Characterisation of vesicular-arbuscular mycorrhizal fungi (Glomus spp) by selective enzyme staining following polyacrylamide gel electrophoresis. Soil Biology and Biochemistry 18: 29-34.
27. Simon L, Lévesque RC, Lalonde M. 1992. Rapid quantification by PCR of endomycorrhizal fungi colonizing roots. PCR Methods and Applications 2: 76-80.
28. Thingstrup I, Rozycka M, Jeffries P, Rosendahl S, Dodd JC. 1995. Detection of the arbuscular mycorrhizal fungus Scutellospora heterogama within roots using polyclonal antisera. Mycological Research 99: 1225-1232.
29. Timonen S, Tammi H, Sen R. 1997. Characterization of the host genotype and fungal diversity in Scots Pine ectomycorrhiza from natural humus microcosms using isozyme and PCR-RFLP analyses. New Phytologist 135: 313-323.
30. Tisserant B, Gianinazzi-Pearson V, Gianinazzi S, Gollotte A. 1993. In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections. Mycological Research 97: 245-250.
31. Trouvelot A, Kough J, Gianinazzi-Pearson V. 1986. Mesure du taux de mycorhization VA d'un système radiculaire. Recherche de méthodes d'estimation ayant une signification fonctionnelle. In: Gianinazzi V, Gianinazzi S, eds. Physiological and Genetical Aspects of Mycorrhizae. Dijon, France: INRA Press, 217-221.
32. van Tuinen D, Dulieu H, Zézé A, Gianinazzi-Pearson V. 1994. Biodiversity and characterization of arbuscular mycorrhizal fungi at the molecular level. In: Gianinazzi S, Schüepp H, eds. Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystem. Basel, Switzerland: Birkhäuser Verlag, 13-24.
33. Walker C, Trappe JM. 1993. Names and epithets in the Glomales and Endogonales. Mycological Research 97: 339-344.
34. Wright SF, Morton JB. 1989. Detection of vesicular-arbuscular mycorrhizal fungus colonization of roots by using a dot-immunoblot assay Applied and Environmental Microbiology 55: 761-763.
35. Wyss P, Bonfante P. 1994. Amplification of genomic DNA of arbuscular-mycorrhizal (AM) fungi by PCR using short arbitrary primers. Mycological Research 97: 1351-1357.