Characterization of the host genotype and fungal diversity in Scots pine ectomycorrhiza from natural humus microcosms using isozyme and PCR-RFLP analyses

New Phytologist

Volumn 135, Issue 2, 1997, Pages 313-323

  Timonen, Sari ^a   Tammi, Hanna ^a   Sen, Robin ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Ectomycorrhiza; Host identification; Isozyme; Microcosm; PCR ITS RFLP

Indexed keywords

EID: 0031440130     PISSN: 0028646X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1469-8137.1997.00645.x     Document Type: Article

References (53)

1. Agerer R. 1995. Anatomical characteristics of identified ectomycorrhizas: an attempt towards a natural classification. In: Varma A, Hock B, eds. Mycorrhiza: Structure, Function, Molecular Biology and Biotechnology. Berlin, Heidelberg: Springer-Verlag, 687-734.
2. Arnebrandt K, Söderström, B. 1992. Effects of different fertiliser treatments on ectomycorrhizal colonisation potential in two Scots pine forests in Sweden. Forest Ecology and Management 53: 77-89.
3. Arnolds E. 1991. Decline of ectomycorrhizal fungi in Europe. Agriculture, Ecosystems and Environment 35: 209-244.
4. Brandrud TE. 1995. The effects of experimental nitrogen addition on the ectomycorrhizal fungus flora in an oligotrophic spruce forest at Gårdsjön, Sweden. Forest Ecology and Management 71: 111-122.
5. Cajander AK. 1926. The theory of forest types. Acta Forestalia Fennica 29: 85-108.
6. Dahlberg A. 1991. Ectomycorrhiza in coniferous forests: structure and dynamics of populations and communities Doctoral thesis. Swedish University of Agricultural Sciences, SLU Info/Repro, Uppsala, Sweden.
7. Dahlberg A. 1995. Somatic incompatibility in ectomycorrhizas. In: Varma A, Hock B, eds. Mycorrhiza: Structure, Function, Molecular Biology and Biotechnology. Berlin, Heidelberg: Springer-Verlag, 115-136.
8. Dahlberg A, Stenlid J. 1994. Size, distribution and biomass of genets in populations of Suillus bovinus revealed by somatic incompatibility. New Phytologist 128: 225-234.
9. Dahlberg A, Stenlid J. 1995. Spatiotemporal patterns in ectomycorrhizal populations. Canadian Journal of Botany 73 (Suppl. 1): S1222-S1230.
10. Dahlberg A, Stenström E. 1991. Dynamic changes in nursery and indigenous ectomycorrhiza of Pinus sylvestris seedlings planted out in forest clearcuts. Plant and Soil 136: 73-86.
11. Deacon JW, Fleming LV. 1992. Interactions of ectomycorrhizal fungi. In: Allen MI ed. Mycorrhizal Functioning. New York: Chapman & Hall, 249-300.
12. Dodd JC, Rosendahl S, Giovannetti M, Broome A, Lanfranco L, Walker C. 1996. Inter- and intraspecific variation within the morphologically-similar arbuscular mycorrhizal fungi Glomus mosseae and Glomus coronatum. New Phytologist 133: 113-122.
13. Erland S. 1995. Abundance of Tylospora fibrillosa ectomycorrhizas in a South Swedish spruce forest measured by RFLP analysis of the PCR-amplified rDNA ITS region. Mycological Research 99: 1425-1428.
14. Erland S, Henrion B, Martin F, Glover LA, Alexander IJ. 1994. Identification of the ectomycorrhizal basidiomycete Tylospora fibrillosa Donk by RFLP analysis of the PCR-amplified ITS and IGS regions of ribosomal DNA. New Phytologist 126: 525-532.
15. Erland S, Söderström B. 1990 Effects of liming on ectomycorrhizal fungi infecting Pinus sylvestris L. I. Mycorrhizal infection in limed humus in the laboratory and isolation of fungi from mycorrhizal roots. New Phytologist 115: 675-682
16. Fellner R, Pesková V. 1995 Effects of industrial pollutants on ectomycorrhizal relationships in temperate forests. Canadian Journal of Botany 73 (Suppl. 1): 1S1310-S1315.
17. Finlay RD, Read DJ. 1986. The structure and function of the vegetative mycelium of ectomycorrhizal plants. II. The uptake and distribution of phosphorus by mycelial strands interconnecting host plants. New Phytologist 103: 157-165.
18. Gardes M, White TJ, Fortin JA, Bruns TD, Taylor JW. 1991. Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA. Canadian Journal of Botany 69: 80-190.
19. Gardes M, Bruns TD. 1993a. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113-118.
20. Gardes M, Bruns TD. 1993 b. Molecular tools for the identification of ectomycorrhizal fungi-taxon-specific oligonucleotide probes for suilloid fungi Molecular Ecology 2: 233-242.
21. Hamrick, JL. 1989. Isozymes and the analysis of genetic structure in plant populations. In: Soltis DE, Soltis PS, eds. Isozymes in Plant Biology. Portland, OR, USA: Dioscorides Press.
22. Harley JL, Smith SE. 1983. Mycorrhizal symbiosis. London: Academic Press.
23. Henrion B, Le Tacon F, Martin F. 1992. Rapid identification of genetic variation of ectomycorrhizal fungi by amplification of ribosomal RNA genes. New Phytologist 122: 289-298.
24. Henrion B, Chevalier G, Martin F. 1993. Typing truffles species by PCR amplification of the ribosomal DNA spacers. Mycological Research 98, 37-43.
25. Henrion B, Di Battista C, Bouchard D, Vairelles D, Thompson BD, Le Tacon F, Martin F. 1994. Monitoring the persistence of Laccaria bicolor as an ectomycorrhizal symbiont of nursery-grown Douglas fir by PCR of the rDNA intergenic spacer. Molecular Ecology 3: 571-580.
26. Hepper CM, Sen R, Azcon-Aguilar C, Grace C. 1988. Variation in certain isozymes amongst different geographical isolates of the vesicular-arbuscular mycorrhizal fungi Glomus clarum, Glomus monosporum and Glomus mosseae. Soil Biology and Biochemistry 20: 51-59.
27. Lehto T. 1994. Effects of soil pH and calcium on mycorrhizas of Picea abies. Plant and Soil 163: 69-75.
28. LoBuglio KF, Rogers SO, Wang CJK. 1991. Variation in ribosomal DNA among isolates of the mycorrhizal fungus Cenococcum geophilum. Canadian Journal of Botany 69: 2331-1343.
29. Martin F, Di Battista C, Delaruelle C, Le Tacon F. 1994. MycorPCR: a molecular database for ectomycorrhizal fungi. In: Abstracts of 4th European Symposium on Mycorrhizas. Granada: Gráficas Alhambra, 22.
30. Marx DH. 1969. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology 59: 153-163.
31. Mason PA, Last FT, Wilson J, Deacon JW, Fleming LV, Fox FM. 1987. Fruiting and successions of ectomycorrhizal fungi. In: Pegg G, Ayres PG, eds. Fungal Infection of Plants, Cambridge: Cambridge University Press, 253-268.
32. Micales JA, Bonde MR, Peterson GL. 1986. The use of isozyme analysis in fungal taxonomy and genetics. Mycotaxon 27: 405-449.
33. Miller SL, Allen EB. 1992. Mycorrhizae, nutrient translocation, and interactions between plants In: Allen MI ed. Mycorrhizal Functioning. New York: Chapman & Hall, 301-332.
34. Muona O, Harju A, Kärkkäinen K. 1988. Genetic comparison of natural and nursery grown seedlings of Pinus sylvestris using allozymes, Scandinavian Journal of Forest Research 3: 37-46.
35. Muona O, Yazdani R, Rudin D. 1987. Genetic change between life stages in Pinus sylvestris: allozyme variation in seeds and planted seedlings Silvae Genetica 36: 39-42.
36. Nylund J-E, Dahlberg A, Högberg N, Kåren, O, Grip K, Jonsson L. 1995. Methods for studying species of mycorrhizal fungal communities in ecological studies and environmental monitoring. In: Stocchi V ed. Biotechnology of Ectomycorrhizae. New York: Plenum Press, 229-239.
37. Ohenoja E. 1988. Behaviour of mycorrhizal fungi in fertilised forests. Karstenia 28: 27-30.
38. Read DJ. 1992. The mycorrhizal mycelium In: Allen MI ed. Mycorrhizal Functioning. New York: Chapman & Hall, 102-113.
39. Read DJ. 1984. The structure and function of the vegetative mycelium of mycorrhizal roots. In: Jennings DH, Rayner ADM, eds. The Ecology and Physiology of the Fungal Mycelium. Cambridge: Cambridge University Press, 215-241.
40. Rosendahl S, Sen R. 1992. Isozyme analysis of mycorrhizal fungi and their ectomycorrhiza. In: Norris JR, Read DJ, Varma AK, eds. Techniques for the Study of Ectomycorrhiza. Methods in Microbiology 24: 167-194.
41. Sambrook J, Fritsch FE, Maniatis T. 1989. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press.
42. Sanders, IR. Alt M, Groppe, K, Boller T, Wiemken A. 1995a. Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytologist 130: 419-427.
43. Sanders IR, Koide RT, Sumway DL. 1995b. Community-level interactions between plants and vesicular-arbuscular mycorrhizal fungi In: Varma A, Hock B, eds. Ectomycorrhiza: Structure, Function, Molecular Biology and Biotechnology. Berlin, Heidelberg: Springer-Verlag, 607-626.
44. Selosse M-A, Costa G, Di Battista C, Le Tacon F, Martin F. 1996. Meiotic segregation and recombination of the intergenic spacer of the ribosomal DNA in the ectomycorrhizal basidiomycete Laccaria bicolor. Current Genetics 30: 332-337.
45. Sen R. 1990a. Intraspecific variation in two species of Suillus from Scots pine (Pinus sylvestris L.) forests based on somatic incompatibility and isozyme analyses. New Phytologist 114: 607-616.
46. Sen R. 1990b. Isozymic identification of individual ectomycorrhizas synthesized between Scots pine (Pinus sylvestris L.) and isolates of two species of Suillus. New Phytologist 114: 617-626.
47. Szmidt AE, Muona O. 1989. Linkage relationships of allozyme loci in Pinus sylvestris L. Hereditas 111: 91-97.
48. Soltis PS, Soltis DE, Doyle JJ. 1992. Molecular systematics of plants. London: Chapman & Hall.
49. Summerbell RC. 1990. Benomyl-tolerant microfungi associated with mycorrhizae of black spruce. Canadian Journal of Botany 66: 553-557.
50. Taylor AFS, Alexander IF. 1990. Demography and population dynamics of ectomycorrhizas fertilised with nitrogen. Agriculture, Ecosystems and Environment 28: 493-496.
51. Timonen S, Finlay RD, Söderström B, Raudaskoski M. 1993. Identification of cytoskeletal components in pine ectomycorrhizas. New Phytologist 124: 83-92.
52. Wendel JF, Weeden NF. 1989. Visualisation and interpretation of plant enzymes. In: Soltis DE, Soltis PS, eds. Isozymes in Plant Biology. Portland, OR, USA: Dioscorides Press, 46-72.
53. White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfaud DH, Sninsky JJ, White TJ, eds. PCR Protocols. A Guide to Methods and Applications. San Diego: Academic Press, 315-322.