Characterization of Neospora caninum iscom antigens using monoclonal antibodies

Parasite Immunology

Volumn 20, Issue 2, 1998, Pages 73-80

  Bjorkman C ^a   Hemphill, A ^b  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Dense granules; Iscom; Monoclonal antibodies; Neospora caninum; Surface proteins

Indexed keywords

EPITOPE; ISCOM; MONOCLONAL ANTIBODY; PARASITE ANTIGEN; PROTOZOAL PROTEIN;

ARTICLE; COCCIDIA; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOBLOTTING; IMMUNOFLUORESCENCE TEST; IMMUNOGOLD STAINING; NEOSPORA CANINUM; NONHUMAN; PRIORITY JOURNAL; PROTOZOAL INFECTION; SERODIAGNOSIS; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, PROTOZOAN; EPITOPES; MICE; MICROSCOPY, ELECTRON; NEOSPORA;

BOS TAURUS; COCCIDIA; NEOSPORA CANINUM; PROTOZOA;

EID: 0031978641     PISSN: 01419838     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3024.1998.00127.x     Document Type: Article

References (26)

1. Anderson M.L., Blanchard P.C. & Barr B.C. et al. (1991) Neospora-like protozoan infection as a major cause of abortion in California dairy cattle. Journal of American Veterinary Medical Association 198, 241-244
2. Barta J.R. & Dubey J.P. (1992) Characterization of anti-Neospora caninum hyperimmune rabbit serum by Western blot analysis and immunoelectron microscopy. Parastology Research 78, 689-694
3. Baszler T.V., Knowles D.P. & Dubey J.P. et al. (1996) Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 34, 1423-1428
4. Bjerkås I., Jenkins M.C. & Dubey J.P. (1994) Identification and characterization of Neospora caninum tachyzoite antigens useful for diagnosis of neosporosis. Clinical and Diagnostic Laboratory Immunology 1, 214-221
5. Björkman C., Holmdahl O.J.M. & Uggla A. (1997) An indirect enzyme-linked immunoassay (ELISA) for demonstration of antibodies to Neospora caninum in serum and milk of cattle. Veterinary Parasitology 68, 251-226
6. Björkman C., Lundén A. & Holmdahl J. et al. (1994) Neospora caninum in dogs: detection of antibodies by ELISA using an iscom antigen. Parasite Immunology 16, 643-648
7. Conrad P.A., Sverlow K. & Anderson M. et al. (1993) Detection of serum antibody responses in cattle with natural or experimental Neospora infections. Journal of Veterinary Diagnosis and Investigations 5, 572-578
8. Dubey J.P., Carpenter J.L. & Speer C.A. et al. (1988) Newly recognized fatal protozoan disease of dogs. Journal of the American Veterinary Medical Association 192, 1269-1285
9. Dubey J.P. & Lindsay D.S. (1993) Neosporosis. Parasitology Today 9, 452-458
10. Dubey J.P. & Lindsay D.S. (1996) A review of Neospora caninum and neosporosis. Veterinary Parasitology 67, 1-59
11. Harlow E. & Lane D. (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, New York
12. Hemphill A. (1996) Subcellular localisation and functional characterization of Nc-p43, a major Neospora caninum tachyzoite surface protein. Infection and Immunity 64, 4279-4287
13. Hemphill A. & Croft S.L. (1997) Electron microscopy in parasitology. In Analytical Parasitology, M. T. Rogan, ed. pp. 227-268. Springer Verlag, Heidelberg
14. Hemphill A., Felleisen R. & Connolly B. et al. (1997a) Characterization of a cDNA clone encoding Nc-p43, a major Neospora caninum tachyzoite surface protein. Parasitology (in press)
15. Hemphill A., Fuchs N. & Sonda S. et al. (1997b) Identification and partial characterization of a 36 kDa surface protein on Neospora caninum tachyzoites. Parasitology 115, 371-380
16. Hemphill A. & Gottstein B. (1996) Identification of a major surface protein on Neospora caninum tachyzoites. Parasitology Research 82, 497-504
17. Holmdahl O.J.M. & Mattsson J.G. (1996) Rapid and sensitive identification of Neospora caninum by in vitro amplification of the internal transcribed spacer I, Parasilology 112, 177-182
18. Lally N.C., Jenkins M.C. & Dubey J.P. (1996) Evaluation of two Neospora caninum recombinant antigens for use in an enzyme-linked immunosorbent assay for the diagnosis of bovine neosporosis. Clinical and Diagnostic Laboratory Immunology 3, 275-279
19. Lindsay D.S. & Dubey J.P. (1989) Immunohistochemical diagnosis of Neospora caninum in tissue sections. American Journal of Veterinary Research 50, 1981-1983
20. Lindsay D.S., Dubey J.P. & Upton S.J. et al. (1990) Serological prevalence of Neospora caninum and Toxoplasma gondii in dogs from Kansas. Journal of the Helminthological Society 57, 86-88
21. Lövgren K., Uggla A. & Morein B. (1987) A new approach to the preparation of a Toxoplasma gondii membrane antigen for use in ELISA. Journal of Veterinary Medicine, B 34, 274-282
22. Lundén A., Lövgren-Bengtsson K. & Sjölander A. et al. (1996) Iscoms in parasitological research. Parasitology Today 12, 320-323
23. Müller N., Zimmerman V. & Hentrich B. et al. (1996) Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. Journal of Clinical Microbiology 34, 2850-2852
24. Paré J., Hietala S.K. & Thurmond M.C. (1995) An enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of Neospora sp. infection in cattle. Journal of Veterinary Diagnosis and Investigations 7, 352-359
25. Sabin A.B. (1941) Toxoplasmic encephalitis in children. Journal of the American Medical Association 116, 801-807
26. Thornton R.N., Thompson E.J. & Dubey J.P. (1991) Neospora abortion in New Zealand cattle. New Zealand Veterinary Journal 39, 129-133