Induction of catalytic activity in proteins by lyophilization in the presence of a transition state analogue

Biotechnology and Bioengineering

Volumn 57, Issue 2, 1998, Pages 211-215

  Slade, Christopher J ^a   Vulfson, Evgeny N ^a  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

Author keywords

lactoglobulin; Bovine serum albumin; Catalysis; Conformational memory; Enzyme; Molecular imprinting; Organic solvent; Protein; Transition state analogue

Indexed keywords

AMINES; CATALYSIS; CONFORMATIONS; ENZYME KINETICS; MOLECULAR DYNAMICS; ORGANIC SOLVENTS; SUBSTRATES;

BOVINE SERUM ALBUMIN; LACTOGLOBULIN; LYOPHILIZATION; MOLECULAR IMPRINTING; TRANSITION STATE ANALOG;

CATALYST ACTIVITY;

BETA LACTOGLOBULIN; BOVINE SERUM ALBUMIN; ORGANIC SOLVENT;

ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; FREEZE DRYING; PROTEIN CONFORMATION;

ALKYLATION; ANIMALS; BINDING SITES; BIOTECHNOLOGY; CATALYSIS; CATTLE; FREEZE DRYING; OXIDATION-REDUCTION; PROTEIN CONFORMATION; PROTEINS; SERUM ALBUMIN, BOVINE;

BOVINAE;

EID: 0031973767     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980120)57:2<211::AID-BIT9>3.0.CO;2-Q     Document Type: Article

References (20)

1. Aschaffenburg, R., Drewry, J. 1957. Improved method for the preparation of crystalline-β-lactoglobulin and α-lactalbumin from cow's milk. Biochem. J. 65: 273-277.
2. Beach, J. V., Shea, K. 1994. Designed catalysts: A synthetic network polymer that catalyzes the dehydrofluorination of 4-fluoro-4-(p-nitrophenyl) butan-2-one. J. Am. Chem. Soc. 116: 379-380.
3. Braco, L., Dabulis, K., Klibanov, A. M. 1990. Production of abiotic receptors by molecular imprinting of proteins. Proc. Natl. Acad. Sci. USA 87: 274-277.
4. Dabulis, K., Klibanov, A. M. 1992. Molecular imprinting of proteins and other macromolecules resulting in new adsorbents. Biotechnol. Bioeng. 39: 176-185.
5. Dabulis, K., Klibanov, A. M. 1993. Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants. Biotechnol. Bioeng. 41: 566-571.
6. Hollfelder, F., Kirby, J. A., Tawfik, D. S. 1996. Off-the-shelf proteins that rival tailor-made antibodies as catalysts. Nature 383: 60-63.
7. + as coenzyme in high concentrations of acetonitrile. Eur. J. Biochem. 227: 551-555.
8. Khmelnitsky, Yu, L., Welch, S. H., Clark, D. S., Dordick, J. S. 1994. Salts dramatically enhance activity of enzymes suspended in organic solvents. J. Am. Chem. Soc. 116: 2647-2648.
9. Klibanov, A. M. 1989. Enzymatic catalysis in anhydrous organic solvents. Trends Biochem. Sci. 14: 141-144.
10. Klibanov, A. M. 1995. Enzyme memory: What is remembered and why? Nature 374: 536.
11. Lerner, R. A., Benkovic, S. J., Schultz, P. G. 1991. At the crossroads of chemistry and immunology: Catalytic antibodies. Science 252: 659-667.
12. Mingarro, I., Abad, C., Branco, L. 1995. Interfacial activation-based molecular bioimprinting of lipolytic enzymes. Proc. Natl. Acad. Sci. USA 92: 3308-3312.
13. Muller, R., Andersson, L. A., Mosbach, K. 1993. Molecularly imprinted polymers facilitating β-elimination. Macromol. Chem. Rapid Commun. 14: 637-641.
14. O'Rich, J., Dordick, J. S. 1997. Controlling subtilisin activity and selectivity in organic media by imprinting with nucleophilic substrates. J. Am. Chem. Soc. 119: 3245-3252.
15. Shokat, K. M., Leumann, C. J., Sugasawara, R., Shultz, P. G. 1989. A new strategy for the generation of catalytic antibodies. Nature 338: 269-271.
16. Shokat, K. M., Uno, T., Shultz, P. G. 1994. Mechanistic studies of an antibody-catalyzed elimination reaction. J. Am. Chem. Soc. 116: 2261-2270.
17. Stahl, M., Mansson, M.-O., Mosbach, K. 1990. The synthesis of D-amino ester in an organic medium with α-chymotrypsin modified by bioimprinting procedure. Biotech. Lett. 12: 161-166.
18. Stahl, M., Jeppsson-Wistrand, U., Mansson, M.-O., Mosbach, K. 1991. Induced stereoselectivity of bio-imprinted chymotrypsin in anhydrous organic media. J. Am. Chem. Soc. 113: 9366-9368.
19. Thorn, S. N., Daniels, R. G., Auditor, M.-T. M., Hilvert, D. 1995. Large rate accelerations in antibody catalysis by strategic use of haptenic charge. Nature 373: 228-230.
20. Uno, T., Shultz, P. 1992. An antibody-catalyzed dehydration reaction. J. Am. Chem. Soc. 114: 6537-6574.