Fundamentals of quality assessment of molecular amplification methods in clinical diagnostics

Clinical Chemistry

Volumn 44, Issue 1, 1998, Pages 12-26

  Neumaier, Michael ^a   Braun, Andreas ^b   Wagener, Christoph ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b SEQUENOM INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BIOTECHNOLOGY; CONTAMINATION; DNA DETERMINATION; DNA SYNTHESIS; EQUIPMENT; GENE AMPLIFICATION; GOOD LABORATORY PRACTICE; JOB ANALYSIS; LABORATORY DIAGNOSIS; MOLECULAR BIOLOGY; QUALITY CONTROL; REPRODUCIBILITY;

EID: 0031973351     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/44.1.12     Document Type: Article

References (21)

1. Yap EP, McGee JO. Short PCR product yields improved by lower denaturation temperatures. Nucleic Acids Res 1991;19:1713.
2. Mazars GR, Moyret C, Jeanteur P, Theillet CG. Direct sequencing by thermal asymmetric PCR. Nucleic Acids Res 1991:19:4783.
3. Prince AM, Andrus L. PCR. How to kill unwanted DNA. BioTechniques 1992;12:358-60.
4. Fairfax MR, Metcalf MA, Cone RW. Slow inactivation of dry PCR templates by UV light. PCR Methods Appl 1991;1:142-3.
5. Sarkar G, Sommer SS. Parameters affecting susceptibility of PCR contamination to UV inactivation. BioTechniques 1990;9:590-4.
6. Ou CY, Moore JL, Schochetman G. Use of UV irradiation to reduce false positivity in polymerase chain reaction. BioTechniques 1991;10:442-6.
7. Higuchi R. Rapid and efficient extraction for PCR from cells or blood. Amplifications 1989;2:1-3.
8. Chomczynski P, Sacchi N. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-9.
9. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.
10. Beutler E, Gelbart T, Kuhl W. Interference of heparin with the polymerase chain reaction. BioTechniques 1990;9:166.
11. Imai H, Yamada O, Morita S, Suehiro S, Kurimura T. Detection of HIV-1 RNA in heparinized plasma of HIV-1 seropositive individuals. J Virol Methods 1992;36:181-4.
12. Tsai M, Miyamoto M, Tam SY, Wang ZS, Galli SJ. Detection of mouse mast cell-associated protease mRNA: heparinase treatment greatly improves RT-PCR of tissues containing mast cell heparin. Am J Pathol 1995;146:335-43.
13. Jung R, Lübcke C, Wagener C, Neumaier M. Reversal of RT-PCR inhibition observed in heparinized clinical specimens. BioTechniques 1997;23:24-8.
14. Gillespie HH. Dissolve and capture: a strategy for analysing mRNA in blood. Nature 1994;367:390-1.
15. Greer CE, Peterson SL, Kiviat NB, Manos MM. PCR amplification from paraffin-embedded tissues: effects of fixative and fixation time. Am J Clin Pathol 1990;95:117-24.
16. Schmidt TM, Pace B, Pace NR. Detection of DNA contamination in Taq polymerase. BioTechniques 1991;11:176-7.
17. Longo MC, Berninger MS, Hartley JL. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reaction. Gene 1990;93:125-8.
18. Cimino GD, Metchette KC, Tessman JW, Hearst JE, Isaacs ST. Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction. Nucleic Acids Res 1991; 19:99-107.
19. Isaacs ST, Tessman JW, Metchette KC, Hearst JE, Cimino GD. Post-PCR sterilization: development and application to an HIV-1 diagnostic assay. Nucleic Acids Res 1991;19:109-16.
20. Braun A, Kofler A, Morawietz S, Cleve H. Sequence and organization of the human vitamin D-binding protein gene. Biochim Biophys Acta 1993;1216:385-94.
21. Keith DH, Singer-Sam J, Riggs AD. Active X chromosome DNA is unmethylated at eight CCGG sites clustered in a guanine-plus-cytosine-rich island at the 5′ end of the gene for phosphoglycerate kinase. Mol Cell Biol 1986;6:4122-5.