The requirement for DsbA in pullulanase secretion is independent of disulphide bond formation in the enzyme

Molecular Microbiology

Volumn 27, Issue 3, 1998, Pages 661-667

  Sauvonnet, Nathalie ^a   Pugsley, Anthony P ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PULLULANASE;

ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; DISULFIDE BOND; ELECTROPHORETIC MOBILITY; ENZYME RELEASE; GRAM NEGATIVE BACTERIUM; IMMUNOPRECIPITATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ARTIFICIAL GENE FUSION; AUTORADIOGRAPHY; CYSTEINE; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASE K; GLYCOSIDE HYDROLASES; GRAM-NEGATIVE BACTERIA; KLEBSIELLA; METHIONINE; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; PROTEIN DISULFIDE-ISOMERASE; SERINE; SULFUR RADIOISOTOPES;

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0031963667     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.00722.x     Document Type: Article

References (27)

1. Bortoli-German, I., Brun, E., Py, B., Chippaux, M., and Barras, F. (1994) Periplasmic disulphide bond formation is essential for cellulase secretion by the plant pathogen Erwinia chrysanthemi. Mol Microbiol 11: 545-553.
2. Braun, P., Tommassen, J., and Filloux, A. (1996) Role of the propeptide in folding and secretion of elastase of Pseudomonas aeruginosa. Mol Microbiol 19: 297-306.
3. Brumlik, M.J., van der Goot, F.G., Wong, K.R., and Buckley, T.J. (1997) The disulfide bond in the Aeromonas hydrophila lipase/acyltransferase stabilizes the structure but is not required for secretion or activity. J Bacteriol 179: 3116-3121.
4. Guilvout, I., Carniel, E., and Pugsley, A.P. (1995) Yersinia spp. HMWP2, a cytosolic protein with a cryptic internal signal sequence which can promote alkaline phosphatase export. J Bacteriol 177: 1780-1787.
5. Hardie, K.R., Schulze, A., Parker, M.W., and Buckley, J.T. (1995) Vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation. Mol Microbiol 17: 1035-1044.
6. Hirst, T.R., and Holmgren, J. (1987) Conformation of protein secreted across bacterial outer membanes: a study of enterotoxin translocation from Vibrio choleras. Proc Natl Acad Sci USA 84: 7418-7422.
7. Jacob-Dubuisson, F., Pinkner, J., Xu, Z., Striker, R., Padmanhaban, A., and Hultgren, S. (1994) PapD chaperone function in pilus biogenesis depends on oxidant and chaperone-like activities of DsbA. Proc Natl Acad Sci USA 91: 11552-11556.
8. Kornacker, M.G., and Pugsley, A.P. (1989) Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsiella pneumoniae UNF5023. Mol Microbiol 4: 73-85.
9. Kunkel, T.A., Roberts, J.D., and Zakour, R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol 154: 367-382.
10. Lu, H.-M., Motley, S.T., and Lory, S. (1997) Interactions of the components of the general secretion pathway: role of Pseudomonas aeruginosa type IV pilin subunits in complex formation and extracellular protein secretion. Mol Microbiol 25: 247-259.
11. McIver, K.S., Kessler, E., Olson, J.C., and Ohman, D.E. (1995) The elastase propeptide functions as an intramolecular chaperone required for elastase activity and secretion in Pseudomonas aeruginosa. Mol Microbiol 18: 877-889.
12. Michaelis, S., Chapon, C., d'Enfert, C., Pugsley, A.P., and Schwartz, M. (1985) Characterization and expression of the structural gene for pullulanase, a maltose-inducible secreted protein of Klebsiella pneumoniae. J Bacteriol 164: 633-638.
13. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
14. Poquet, I., Faucher, D., and Pugsley, A.P. (1993) Long-lived periplasmic intermediate in the general secretory pathway in Escherichia coli. EMBO J 12: 271-278.
15. Possot, O., and Pugsley, A. (1997) The conserved tetracysteine motif in the general secretory pathway component PuIE is required for efficient pullulanase secretion. Gene 192: 45-50.
16. Possot, O., Letellier, L., and Pugsley, A.P. (1997) Energy requirement for pullulanase secretion by the main terminal branch of the general secretory pathway. Mol Microbiol 24: 457-464.
17. Pugsley, A.P. (1992) Translocation of a folded protein across the outer membrane via the general secretory pathway in Escherichia coli. Proc Natl Acad Sci USA 89: 12058-12062.
18. Pugsley, A.P. (1993a) The complete general secretory pathway in Gram-negative bacteria. Microbiol Rev 57: 50-108.
19. Pugsley, A.P. (1993b) Processing and methylation of PulG, a pilin-like component of the general secretory pathway of Klebsiella oxytoca. Mol Microbol 9: 295-308.
20. Pugsley, A.P., Chapon, C., and Schwartz, M. (1986) Extracellular pullulanase of Klebsiella pneumoniae is a lipoprotein. J Bacteriol 166: 1083-1088.
21. Pugsley, A.P., Poquet, I., and Kornacker, M.G. (1991a) Two distinct steps in pullulanase secretion by Escherichia coli K12. Mol Microbiol 5: 865-873.
22. Pugsley, A.P., Kornacker, M.G., and Poquet, I. (1991b) The general secretion pathway is directly required for extracellular pullulanase secretion in Escherichia coli. Mol Microbiol 5: 343-352.
23. Sauvonnet, N., and Pugsley, A.P. (1996) Identification of two regions of Klebsiella oxytoca pullulanase that together are capable of promoting β-lactamase secretion by the general secretory pathway. Mol Microbiol 22: 1-7.
24. Sauvonnet, N., Poquet, I., and Pugsley, A.P. (1995) Extracellular secretion of pullulanase is unaffected by minor sequence changes but is usually prevented by adding reporter proteins to its N- or C-terminal end. J Bacteriol 177: 5238-5246.
25. Shevchik, V.E., Bortoli-German, I., Robert-Baudouy, J., Robinet, S., Barras, F., and Condemine, G. (1995) Differential effect of dsbA and dsbC mutations on extracellular enzyme secretion in Erwinia chrysanthemi. Mol Microbiol 16: 745-753.
26. Shevchik, V.E., Robert-Badouy, J., and Condemine, G. (1997) Specific interaction between OutD, an Erwinia chrysanthemi outer membrane protein of the general secretory pathway, and secreted proteins. EMBO J 16: 3007-3016.
27. Wandersman, C. (1996) Secretion across the bacterial outer membrane. In Escherichia coli and Salmonella. Cellular and Molecular Biology. Neidhardt, F.C., Curtiss, III, R., Ingraham, J.L., Lin, E.C.C., Low, K.B., Magasanik, B., et al. (eds). Washington DC: American Society for Microbiology Press, pp. 955-966.