The disulfide bond in the Aeromonas hydrophila lipase/acyltransferase stabilizes the structure but is not required for secretion or activity

Journal of Bacteriology

Volumn 179, Issue 10, 1997, Pages 3116-3121

  Brumlik, Michael J ^a   Van Der Goot, F Gisou ^b   Wong, Kevin R ^c   Buckley, J Thomas ^a  

^a UNIVERSITY OF VICTORIA   (Canada)

^b UNIVERSITY OF GENEVA   (Switzerland)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; TRIACYLGLYCEROL LIPASE;

AEROMONAS HYDROPHILA; ARTICLE; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME STABILITY; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL;

AEROMONAS; AEROMONAS HYDROPHILA; AEROMONAS SALMONICIDA; MAMMALIA; VIBRIO;

EID: 0030922620     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.10.3116-3121.1997     Document Type: Article

References (42)

1. Bortoli-German, I., E. Brun, B. Py, M. Chippaux, and F. Barras. 1994. Periplasmic disulphide bond formation is essential for cellulase secretion by the plant pathogen Erwinia chrysanthemi. Mol. Microbiol. 11:545-553.
2. Brick, D. J., M. J. Brumlik, J. T. Buckley, J.-X. Cao, P. C. Davies, S. Misra, T. J. Tranbarger, and C. Upton. 1995. A new family of lipolytic plant enzymes with members in rice, arabidopsis and maize. FEBS Lett. 377:475-480.
3. Brumlik, M. J., and J. T. Buckley. 1996. Identification of the catalytic triad of the lipase/acyltransferase from Aeromonas hydrophila. J. Bacteriol. 178: 2060-2064.
4. Buckley, J. T. 1982. Substrate specificity of bacterial glycerophospholipid: cholesterol acyltransferase. Biochemistry 21:6699-6703.
5. Buckley, J. T. 1990. Purification of cloned proaerolysin released by a low protease mutant of Aeromonas salmonicida. Biochem. Cell Biol. 68:221-224.
6. Chihara-Siomi, M., K. Yoshikawa, N. Oshima-Hirayama, K. Yamamoto, Y. Sogabe, T. Nakatani, T. Nishioka, and J. Oda. 1992. Purification, molecular cloning, and expression of lipase from Pseudomonas aeruginosa. Arch. Biochem. Biophys. 296:505-513.
7. Darveau, R., S. MacIntyre, J. T. Buckley, and R. E. W. Hancock. 1983. Purification and reconstitution in lipid bilayer membranes of an outer membrane, pore-forming protein of Aeromonas salmonicida. J. Bacteriol. 156: 1006-1011.
8. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
9. Francone, O. L., and C. J. Fielding. 1991. Effects of site-directed mutagenesis at residues cysteine-31 and cysteine-184 on lecithin-cholesterol acyltransferase activity. Proc. Natl. Acad. Sci. USA 88:1716-1720.
10. Frenken, L. G. J., J. W. Bos, C. Visser, W. Müller, J. Tommassen, and C. T. Verrips. 1993. An accessory gene, lipB, required for the production of active Pseudomonas glumae lipase. Mol. Microbiol. 9:579-589.
11. Furste, J. P., W. Pansgreau, R. Frank, H. Blocker, P. Scholz, M. Bagdasarian, and E. Lanka. 1986. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48:119-131.
12. Hardie, K. R., A. Schulze, M. W. Parker, and J. T. Buckley. 1995. Vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation. Mol. Microbiol. 17:1035-1044.
13. Hilton, S., and J. T. Buckley. 1991. Action of a microbial lipase/acyltransferase on phospholipid monolayers. Biochemistry 30:6070-6074.
14. Hilton, S., and J. T. Buckley. 1991. Studies on the reaction mechanism of a microbial lipase/acyltransferase using chemical modification and site-directed mutagenesis. J. Biol. Chem. 266:997-1000.
15. Hilton, S., W. D. McCubbin, C. M. Kay, and J. T. Buckley. 1990. Purification and spectral study of a microbial fatty acyltransferase: activation by limited proteolysis. Biochemistry 29:9072-9078.
16. Hirst, T. R., and J. Holmgren. 1987. Conformation of proteins secreted across bacterial outer membranes: a study of enterotoxin translocation from Vibrio cholerae. Proc. Natl. Acad. Sci. USA 84:7418-7422.
17. Howard, S. P., and J. T. Buckley. 1985. Protein export by a gram-negative bacterium: production of aerolysin by Aeromonas hydrophila. J. Bacteriol. 161:1118-1124.
18. Ihara, F., I. Okamoto, T. Hihira, and Y. Yamada. 1992. Requirement in trans of the downstream limL gene for activation of lactonizing lipase from Pseudomonas sp. 109. J. Ferment. Bioeng. 73:337-342.
19. Jiang, B., and S. P. Howard. 1991. Mutagenesis and isolation of Aeromonas hydrophila genes which are required for extracellular secretion. J. Bacteriol. 173:1241-1249.
20. Jorgensen, S., K. W. Skov, and B. Diderichsen. 1991. Cloning, sequencing, and expression of a lipase from Pseudomonas cepacia: lipase production in heterologous hosts requires two Pseudomonas genes. J. Bacteriol. 173:559-567.
21. Kunkel, T. A. 1985. Rapid and specific site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
22. Liss, L. 1987. New M13 host: DH5αF′ competent cells. Focus 9:13.
23. Lo, J. Y., L. C. Smith, and L. Chan. 1995. Lipoprotein lipase: role of intramolecular disulphide bonds in enzyme catalysis. Biochem. Biophys. Res. Commun. 206:266-271.
24. Miller, J. H. 1972. Experiments in molecular genetics, p. 341. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Nerland, A. H. 1993. EMBL accession number X70686.
26. Neville, D. M. 1971. Molecular weight determination of protein dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J. Biol. Chem. 246:6328-6334.
27. Okamoto, K., T. Baba, Y. Yamanaka, N. Akashi, and Y. Fujii. 1995. Disulfide bond formation and secretion of Escherichia coli beat-stable enterotoxin II. J. Bacteriol. 177:4579-4586.
28. Peek, J. A., and R. K. Taylor. 1992. Characterization of a periplasmic thiol: disulphide interchange protein required for the functional maturation of secreted virulence factors of Vibrio cholerae. Proc. Natl. Acad. Sci. USA 89:6210-6214.
29. Pugsley, A. P. 1992. Translocation of a folded protein across the outer membrane in Escherichia coli. Proc. Natl. Acad. Sci. USA 89:12058-12062.
30. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
31. Qu, S. J., H. Z. Fan, F. Blancovaca, and H. J. Pownall. 1993. Roles of cysteines in human lecithin-cholesterol acyltransferase. Biochemistry 32:3089-3094.
32. 24) heat shock sigma factor of Escherichia coli. EMBO J. 14: 1043-1055.
33. Robertson, D. L., S. Hilton, K. R. Wong, A. Koepke, and J. T. Buckley. 1994. Influence of active site and tyrosine modification on the secretion and activity of the Aeromonas hydrophila lipase/acyltransferase. J. Biol. Chem. 269: 2146-2150.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
35. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
36. Singh, R., and G. M. Whitesides. 1995. Separations and other methods, p. 259-266. In J. W. Crabb (ed.), Techniques in protein chemistry VI. Academic Press, New York, N.Y.
37. Strom, M. S., D. Nunn, and S. Lory. 1991. Multiple roles of pilus biogenesis protein PilD: involvement of PilD in excretion of enzymes from Pseudomonas aeruginosa. J. Bacteriol. 173:1175-1180.
38. Upton, C., and J. T. Buckley. 1995. A new family of lipolytic enzymes? Trends Biochem. Sci. 233:178-179.
39. van der Goot, F. G., J. Ausio, K. R. Wong, F. Pattus, and J. T. Buckley. 1993. Dimerization stabilizes the pore-forming toxin aerolysin in solution. J. Biol. Chem. 268:18272-18279.
40. Wong, K. R., and J. T. Buckley. 1989. Proton motive force involved in protein transport across the outer membrane of Aeromonas salmonicida. Science 246:654-656.
41. Wong, K. R., and J. T. Buckley. Aeromonas spp. can secrete Escherichia coli alkaline phosphatase into the culture supernatant, and its release requires a functional general secretion pathway. Mol. Microbiol. 9:955-963.
42. Wong, K. R., M. J. Green, and J. T. Buckley. 1989. Extracellular secretion of cloned aerolysin and phospholipase by Aeromonas salmonicida. J. Bacteriol. 171:2523-2527.