Isolation of a non-classical mutant of the DNA recognition subunit of the Type I restriction endonuclease R.EcoR124I

Biological Chemistry

Volumn 379, Issue 4-5, 1998, Pages 585-589

  Weiserova, Marie ^a   Firman, Keith ^b  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^b UNIVERSITY OF PORTSMOUTH   (United Kingdom)

Author keywords

Misincorporation mutagenesis; PCR; Protein protein interactions; Restriction modification; Subunit assembly

Indexed keywords

ARGININE; DNA; RESTRICTION ENDONUCLEASE; TRYPTOPHAN;

AMINO ACID SUBSTITUTION; CONFERENCE PAPER; CONTROLLED STUDY; DNA METHYLATION; ENZYME SUBUNIT; GENE DELETION; MUTAGENESIS; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; DEOXYRIBONUCLEASES, TYPE I SITE-SPECIFIC; DNA; DNA RESTRICTION-MODIFICATION ENZYMES; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION;

EID: 0031959009     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (29)

1. Abadjieva, A., Patel, J., Webb, M., Zinkevich, V., and Firman, K. (1993). A deletion mutant of the type IC restriction endonuclease EcoR124I expressing a novel DNA specificity. Nucleic Acids Res. 21, 4435-4443.
2. Abadjieva, A., Patel, J., Zinkevich, V., and Firman, K. (1994). Deletions within the DNA recognition subunit of M. EcoR124I that identify a region involved in protein protein interactions between HsdS and HsdM. J. Mol. Biol. 241, 35-43.
3. Arcangioli, B., Ghazvini, M., and Ribes, V. (1994). Identification of the DNA-binding domains of the switch-activating-protein Sap1 from S. pombe by random point mutations screening in E. coli. Nucleic Acids Res. 22, 2930-2937.
4. Belogurov, A.A., and Delver, E.P. (1995). A motif conserved among the type I restriction-modification enzymes and antirestriction proteins: a possible basis for mechanism of action of plasmid-encoded antirestriction functions. Nucleic Acids Res. 23, 785-787.
5. Bickle, T.A., and Krüger, D.H. (1993). Biology of DNA restriction. Microbiol. Rev. 57, 434-450.
6. Cooper, L.P., and Dryden, D.T.F. (1994). The domains of a type I DNA methyltransferase. Interactions and role in recognition of DNA methylation. J. Mol. Biol. 236, 1011-1021.
7. Cowan, G.M., Daniel, A.S., Gann, A.A.F., Kelleher, J.E., and Murray, N.E. (1988). Defining domains in type-I restriction and modification enzymes. Gene 74, 239-241.
8. Cowan, G.M., Gann, A.A.F., and Murray, N.E. (1989). Conservation of complex DNA recognition domains between families of restriction enzymes. Cell 56, 103-109.
9. Dryden, D.T.F., Cooper, L.P., and Murray, N.E. (1993). Purification and characterisation of the methyltransferase from the type I restriction and modification system of Escherichia coli K12. J. Biol. Chem. 268, 13228-13236.
10. Fuller-Pace, F.V., Bullas, L.R., Delius, H., and Murray, N.E. (1984). Genetic recombination can generate altered restriction specificity. Proc. Natl. Acad. Sci. USA 81, 6095-6099.
11. Gann, A.F.F., Campbell, A.J.B., Collins, J.F., Coulson, A.F.W., and Murray, N.E. (1987). Reassortment of DNA recognition domains and the evolution of new specificities. Mol. Microbiol. 1, 13-22.
12. Gilbert, W., and Müller-Hill, B. (1970). The lactose repressor. In: The Lactose Operon. J.F. Beckwith and D. Zipser, eds. (New York, USA: Cold Spring Harbor Laboratory Press), pp. 93-109.
13. Glover, S.W. (1970). Functional analysis of host-specificity mutants in Escherichia coli. Genet. Res. (Cambs) 15, 237-250.
14. Hubacek, J., and Glover, S.W. (1970). Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli. J. Mol. Biol. 50, 111-127.
15. Hubacek, J., Zinkevich, V.E., and Weiserova, M. (1989). The location of a temperature-sensitive trans-dominant mutation and its effect on restriction and modification in Escherichia coli K12. J. Gen. Microbiol. 135, 3057-3065.
16. Janscak, P., Abadjieva, A., and Firman, K. (1996). The type I restriction endonuclease R.EcoR124I: Over-production and biochemical properties. J. Mol. Biol. 257, 977-991.
17. Kannan, P., Cowan, G.M., Daniel, A.S., Gann, A.A.F., and Murray, N.E. (1989). Conservation of organisation in the specificity polypeptides of two families of type I restriction enzymes. J. Mol. Biol. 209, 335-344.
18. Kneale, G.G. (1994). A symmetrical model for the domain structure of type I DNA methyltransferases. J. Mol. Biol. 243, 1-5.
19. MacWilliams, M.P., and Bickle, T.A. (1996). Generation of new DNA binding specificity by truncation of the type IC EcoDXXI hsdS gene. EMBO J. 15 (17), 4775-4783.
20. Meister, J., MacWilliams, M., Hübner, P., Jütte, H., Skrzypek, E., Piekarowicz, A., and Bickle, T.A. (1993). Macroevolution by transposition: drastic modification of DNA recognition by a type I restriction enzyme following Tn5 transposition. EMBO J. 12 (12), 4585-4591.
21. Murray, N.E., Gough, J.A., Suri, B., and Bickle, T.A. (1982). Structural homologies among type I restriction and modification systems. EMBO J. 1, 535-539.
22. Patel, J., Taylor, I., Dutta, C.F., Kneale, G.G., and Firman, K. (1992). High-level expression of the cloned genes encoding the subunits of and the intact DNA methyltransferase, M.EcoR124. Gene 112, 21-27.
23. Price, C., Bickle, T.A., Firman, K., and Glover, S.W. (1989). Unequal crossing over as a mechanism to generate changes in DNA sequence recognition by the type I restriction and modification enzymes. J. Mol. Biol. 205, 115-125.
24. Redaschi, N., and Bickle, T.A. (1996). DNA restriction and modification systems. In: Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edit. F.C. Neidhardt, R. Curtiss III, J.L. Ingraham, E.C.C. Lin, K.B. Low, B. Magasanik and W.S. RedReznikoff, eds. (Washington, DC: American Society for Microbiology), pp. 773-781.
25. Taylor, I., Patel, J., Firman, K., and Kneale, G.G. (1992). Purification and biochemical characterisation of the EcoR124 modification methylase. Nucleic Acids Res. 20, 179-186.
26. Titheradge, A.J.B., Ternent, D., and Murray, N.E. (1996). A third family of allelic hsd genes in Salmonella enterica: sequence comparisons with related proteins identify conserved regions implicated in restriction of DNA. Mol. Microbiol. 22, 437-447.
27. Wilson, G.G. (1991). Organisation of restriction-modification systems. Nucleic Acids Res. 19, 2539-2566.
28. Wilson, G.G., and Murray, N.E. (1991). Restriction and modification systems. Ann. Rev. Genet. 25, 585-627.
29. Zinkevich, V., Heslop, P., Glover, S.W., Weiserova, M., Hubacek, J., and Firman, K. (1992). A mutation in the specificity polypeptide of the type I restriction endonuclease R.EcoK that affects subunit assembly. J. Mol. Biol. 227, 597-601.