Purification of mammalian mitochondrial chaperonin 60 through in vitro reconstitution of active oligomers

Methods in Enzymology

Volumn 290, Issue , 1998, Pages 203-217

  Viitanen, Paul V ^a   Lorimer, George ^a   Bergmeier, Wolfgang ^a   Weiss, Celeste ^a   Kessel, Martin ^a   Goloubinoff, Pierre ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONIN;

AMINO ACID SEQUENCE; ARTICLE; ESCHERICHIA COLI; ION EXCHANGE CHROMATOGRAPHY; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN QUALITY;

ESCHERICHIA COLI; MAMMALIA;

EID: 0031953354     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(98)90020-9     Document Type: Article

References (37)

1. P. V. Viitanen, G. H. Lorimer, R. Seetharam, R. S. Gupta, J. Oppenheim, J. O. Thomas, and N. J. Cowan, J. Biol. Chem. 267, 695 (1992).
2. D. J. Picketts, C. S. K. Mayanil, and R. S. Gupta, J. Biol. Chem. 264, 12001 (1989).
3. S. Jindal, A. K. Dudani, B. Singh, C. B. Harley, and R. S. Gupta, Mol. Cell. Biol. 9, 2279 (1989).
4. R. S. Gupta and R. A. Austin, Eur. J. Cell Biol. 45, 170 (1987).
5. M. Y. Cheng, F.-U. Hartl, J. Martin, R. A. Pollock, F. Kalousek, W. Neupert, E. M. Hallberg, R. L. Hallberg, and A. L. Horwich, Nature (London) 337, 620 (1989).
6. S. Jindal, E. Burke, B. Bettencourt, S. Khandekar, R. A. Young, and D. S. Dwyer, Immunol. Lett. 39, 127 (1994).
7. L. A. Mizzen, C. Chang, J. I. Garrels, and W. J. Welch, J. Biol. Chem. 264, 20664 (1989).
8. S. J. Pilkington and J. E. Walker, DNA Sequencing 3, 291 (1993).
9. M. T. Ryan, N. J. Hoogenraad, and P. B. Høj, FEBS Lett. 337, 152 (1994).
10. R. Dickson, B. Larsen, P. V. Viitanen, M. B. Tormey, J. Geske, R. Strange, and L. T. Bemis, J. Biol. Chem. 269, 26858 (1994).
11. G. Legname, T. Fossati, G. Gromo, N. Monzini, F. Marcucci, and D. Modena, FEBS Lett. 361, 211 (1995).
12. T. H. Lubben, A. A. Gatenby, G. K. Donaldson, G. H. Lorimer, and P. V. Viitanen, Proc. Natl. Acad. Sci. U.S.A. 87, 7683 (1990).
13. D. J. Hartman, N. J. Hoogenraad, R. Condron, and P. B. Høj. Proc. Natl. Acad. Sci. U.S.A. 89, 3394 (1992).
14. M. T. Ryan, D. J. Naylor, N. J. Hoogenraad, and P. B. Høj, J. Biol. Chem. 270, 22037 (1995).
15. A. C. Cavanagh and H. Morton, Eur. J. Biochem. 222, 551 (1994).
16. P. V. Viitanen, K. Bacot, R. Dickson, and T. Webb, Methods Enzymol. 290, Chap. 19 (1998) (this volume).
17. P. V. Viitanen, M. Schmidt, J. Buchner, T. Suzuki, E. Vierling, R. Dickson, G. H. Lorimer, A. Gatenby, and J. Soll, J. Biol. Chem. 270, 18158 (1995).
18. N. M. Lissin, S. Yu Venyaminov, and A. S. Girshovich, Nature (London) 348, 339 (1990).
19. N. M. Lissin, FEBS Lett. 361, 55 (1995).
20. M. Morioka and H. Ishikawa, J. Biochem. 114, 468 (1993).
21. R. S. Boston, P. V. Viitanen, and E. Vierling, Plant Molecular Biology 32, 191 (1996).
22. S. Gutteridge, G. S. Reddy, and G. H. Lorimer, Biochem. J. 260, 711 (1989).
23. M. Schmidt, J. Buchner, M. J. Todd, G. H. Lorimer, and P. V. Viitanen, J. Biol. Chem. 269, 10304 (1994).
24. All pH values reported in text were measured at 25°.
25. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem. 193, 265 (1951).
26. U. K. Laemmli, Nature (London) 227, 680 (1970).
27. Although not to the same extent that is observed with the mammalian mt-cpn60, lower temperatures also destabilize other GroEL homologs (cf. Ref. 19).
28. All buffer exchange steps are carried out at 4° using PD-10 gel-filtration columns (Pharmacia, Piscataway, NJ).
29. The NaCl concentrations cited have been corrected for the 8-ml transit through the column.
30. Unless indicated, protein samples are concentrated at 4° using Centriprep-30 and Centricon-30 concentrators; both are available from Amicon (Danvers, MA).
31. P. V. Viitanen and W. Bergmeier, unpublished observations (1996).
32. M. Kessel and P. V. Viitanen, unpublished observations (1996).
33. N. Ishii, H. Taguchi, H. Sasabe, and M. Yoshida. FEBS Lett. 362, 121 (1995).
34. ATP hydrolysis is not required since "footballs" are also observed with AMP-PNP, but not ADP.
35. M. Schmidt, K. Rutkat, R. Rachel, G. Pfeifer, R. Jaenicke, P. Viitanen, G. Lorimer, and J. Buchner, Science 265, 656 (1994).
36. A. Azem, M. Kessel, and P. Goloubinoff, Science 265, 653 (1994).
37. M. J. Todd, P. V. Viitanen, and G. H. Lorimer, Science 265, 659 (1994).