Characterization of Mycobacterium tuberculosis LexA: Recognition of a Cheo (Bacillus-type SOS) box

Microbiology

Volumn 143, Issue 3, 1997, Pages 929-936

  Movahedzadeh, Farahnaz ^a   Colston, M Joseph ^a   Davis, Elaine O ^a  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

DNA binding protein; DNA damage inducible genes; LexA; Mycobacterium tuberculosis; RecA

Indexed keywords

DNA BINDING PROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; CONSENSUS SEQUENCE; CONTROLLED STUDY; DNA REPAIR; DNA SEQUENCE; ESCHERICHIA COLI; GENETIC CONSERVATION; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TRANSCRIPTION INITIATION;

EID: 0030999727     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-3-929     Document Type: Article

References (36)

1. Brent, R. & Ptashne, M. (1981). Mechanism of action of the lexA gene product. Proc Natl Acad Sci USA 78, 4204-4208.
2. Cheo, D. L., Bayles, K. W. & Yasbin, R. E. (1991). Cloning and characterisation of DNA damage inducible promoter regions from Bacillus subtilis. J Bacteriol 173, 1696-1703.
3. Davis, E. O., Sedgwick, S. G. & Colston, M. J. (1991). Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product. J Bacteriol 173, 5653-5662.
4. Davis, E. O., Thangaraj, H. S., Brooks, P. C. & Colston, M. J. (1994). Evidence of selection for protein introns in the RecAs of pathogenic mycobacteria. EMBO J 13, 699-703.
5. Devereux, J., Haeberli, P. & Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12, 387-395.
6. + NMR spectroscopy. EMBO J 13, 3936-3944.
7. Garriga, X., Calero, S. & Barbe, J. (1992). Nucleotide sequence analysis and comparison of the lexA genes from Salmonella typhimurium, Erwinia carotovora, Pseudomonas aeruginosa and Pseudomonas putida. Mol Gen Genet 236, 125-134.
8. Gonzalez-y-Merchand, J. A., Colston, M. J. & Cox, R. A. (1996). The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis: comparison of promoter elements and of neighbouring upstream genes. Microbiology 142, 667-674.
9. Holmes, D. S. & Quigley, M. (1981). A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114, 193-197.
10. Horii, T., Ogawa, T., Nakatani, T., Hase, T., Matsubara, H. & Ogawa, H. (1981a). Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein. Cell 27, 515-522.
11. Horii, T., Ogawa, T. & Ogawa, H. (1981b). Nucleotide sequence of the lexA gene of E. coli. Cell 23, 689-697.
12. Knegtel, R. M. A., Fogh, R. H., Ottleben, G., Ruterjans, H., Dumoulin, P., Schnarr, M., Boelens, R. & Kaptein, R. (1995). A model for the LexA repressor DNA complex. Proteins 21, 226-236.
13. Kochi, A. (1991). The global tuberculosis situation and the new control strategy of the World Health Organisation. Tubercle 72, 1-6.
14. Little, J. W. (1984). Autodigestion of lexA and phage lambda repressors. Proc Natt Acad Sci USA 81, 1375-1379.
15. Little, J. W. (1991). Mechanism of specific LexA cleavage: autodigestion and the role of RecA coprotease. Biochimie 73, 411-422.
16. Little, J. W. & Mount, D. W. (1982). The SOS regulatory system of Escherichia coli. Cell 29, 11-22.
17. Little, J. W., Kim, B., Roland, K. L., Smith, M. H., Lin, L. L. & Slilaty, S. N. (1994). Cleavage of LexA repressor. Methods Enzymol 244, 266-284.
18. Markham, B. E., Little, J. W. & Mount, D. (1981). Nucleotide sequence of the lexA gene of Escherichia coli K-12. Nucleic Acids Res 9, 4149-4161.
19. Mead, D. A., Szczesna-Skorupa, E. & Kemper, B. (1987). Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering. Protein Eng 1, 67-74.
20. Miller, R. V. & Kokjohn, T. A. (1990). General microbiology of recA: environmental and evolutionary significance. Annu Rev Microbiol 44, 365-394.
21. Mustard, J. A., Thliveris, A. T. & Mount, D. W. (1992). Sequence of the Salmonella typhimurium LT2 lexA gene and its regulatory region. Nucleic Acids Res 20, 1813.
22. Philipp, W. J., Poulet, S., Eiglmeier, K., Pascopella, L., Balasubramanian, V., Heym, B., Bergh, S., Bloom, B. R., Jacobs, W. R. & Cole, S. T. (1996). An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae. Proc Natl Acad Sci USA 93, 3132-3137.
23. Raymond-Denise, A. & Guillen, N. (1991). Identification of dinR, a DNA damage-inducible regulator gene of Bacillus subtilis. J Bacteriol 173, 7084-7091.
24. Riera, J. & Barbe, J. (1993). Sequence of the Providencia rettgeri lexA gene and its control region. Nucleic Acids Res 21, 2256.
25. Riera, J. & Barbe, J. (1995). Cloning, sequence and regulation of expression of the lexA gene of Aeromonas hydrophila. Gene 154, 71-75.
26. Rosenberg, M. & Court, D. (1979). Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13, 319-353.
27. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
28. Sarkis, G. J., Jacobs, W. R. & Hatfull, G. F. (1995). L5 luciferase reporter mycobacteriophages: a sensitive tool for the detection and assay of live mycobacteria. Mol Microbiol 15, 1055-1067.
29. Slilaty, S. N. & Little, J. W. (1987). Lysine-156 and serine-119 are required for LexA repressor cleavage: a possible mechanism. Proc Nad Acad Sci USA 84, 3987-3991.
30. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185, 60-89.
31. Suzuki, M., Yagi, N. & Gerstein, M. (1995). DNA recognition and superstructure formation by helix-turn-helix proteins. Protein Eng 8, 329-338.
32. Thliveris, A. T. & Mount, D. W. (1992). Genetic identification of the DNA binding domain of Escherichia coli LexA protein. Proc Natl Acad Sci USA 89, 4500-4504.
33. Thliveris, A. T., Little, J. W. & Mount, D. W. (1991). Represssion of the E. coli recA gene requires at least two LexA protein monomers. Biochimie 73, 449-455.
34. Timm, J., Perilli, M. G., Duez, C., Trias, J., Orefici, G., Fattorini, L., Amicosante, G., Oratore, A., Joris, B., Frere, J. M., Pugsley, A. P. & Gicquel, B. (1994). Transcription and expression analysis, using lacZ. and phoA gene fusions, of Mycobacterium fortuitum β-lactamase genes cloned from a natural isolate and a high-level β-lactamase producer. Mol Microbiol 12, 491-504.
35. Walker, G. C. (1984). Mutagenesis and inducible responses to deoxyribonucleic acid damage in E. coli. Microbiol Rev 48, 60-93.
36. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.