LNCap produces both putative zymogen and inactive, free form of prostate-specific antigen

Prostate

Volumn 35, Issue 2, 1998, Pages 135-143

  Corey, Eva ^a   Brown, Lisha G ^a   Corey, Michael J ^a   Buhler, Kent R ^a   Vessella, Robert L ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

Free PSA; LNCaP; Prostate adenoma; Prostate specific antigen; PSA ACT complex

Indexed keywords

ALPHA 1 ANTICHYMOTRYPSIN; ENZYME PRECURSOR; PROSTATE SPECIFIC ANTIGEN; TRYPSIN;

ANTIGEN EXPRESSION; ARTICLE; CANCER CELL CULTURE; COMPLEX FORMATION; ENZYME ACTIVATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROSTATE CANCER; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALPHA 1-ANTICHYMOTRYPSIN; BLOTTING, WESTERN; DNA PRIMERS; ENZYME PRECURSORS; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; PEPTIDES; POLYMERASE CHAIN REACTION; PROSTATE-SPECIFIC ANTIGEN; PROSTATIC NEOPLASMS; PROSTATIC SECRETORY PROTEINS; RNA, MESSENGER; RNA, NEOPLASM; RNA-DIRECTED DNA POLYMERASE; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 0031942975     PISSN: 02704137     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0045(19980501)35:2<135::AID-PROS7>3.0.CO;2-I     Document Type: Article

References (28)

1. Christensson A, Laurell C-B, Lilja H: Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors. Eur J Biochem 1990;194:755-763.
2. Leinonen J, Zhang WM, Stenman UH: Complex formation between PSA isoenzymes and protease inhibitors. J Urol 1996;155: 1099-1103.
3. Zhang WM, Lienonen J, Stenman UH: Prostate-specific antigen (PSA) complexes with and cleaves alpha-1-protease inhibitor in vitro. J Urol [Suppl] 1996;155:1259.
4. Christensson A, Lilja H: Complex formation between protein C inhibitor and prostate-specific antigen in vitro and in human semen. Eur J Biochem 1994;220:45-53.
5. Lilja H, Christensson A, Dahlen U, Matikainen M, Nilsson O, Pettersson K, Lovgren T: Prostate-specific antigen in serum occurs predominantly in complex with alpha-antichymotrypsin. Clin Chem 1991;37:1618-1625.
6. Stenman UH, Leinonen J, Alfthan H, Rannikko S, Tuhkanen K, Alfthan O: A complex between prostate-specific antigen and α-antichymotrypsin is the major form of prostate-specific antigen in serum of patients with prostatic cancer: Assay of the complex improves clinical sensitivity for cancer. Cancer Res 1991;5:222-226.
7. Christensson A, Bjork T, Nilsson O, Dahlen U, Matikainen M-T, Cockett ATK, Abrahamsson P-A, Lilja H: Serum prostate-specific antigen complexed to alpha-1-antichymotrypsin as an indicator of prostate cancer. J Urol 1993;150:100-105.
8. Bjork R, Bjartell A, Abrahamsson PA, Hulkko S, di Sant'Agnese A, Lilja H: Alpha-antichymotrypsin production in PSA-producing cells is common in prostate cancer but rare in benign prostatic hyperplasia. Urology 1994;43:427-434.
9. Bjartell A, Bjork T, Matikainen MT, Abrahamsson PA, di Sant'Agnese A, Lilja H: Production of alpha-1-antichymotrypsin by PSA-producing cells of human prostate epithelium. Urology 1993;42:502-510.
10. Killian CS, Corral DA, Kawinski E, Constantine RI: Mitogenic response of osteoblast cells to prostate-specific antigen suggests an activation of latent TGF-β and a proteolytic modulation of cell adhesion receptors. Biochem Biophys Res Commun 1993; 192:940-947.
11. Plymate SR, Rosen CJ, Paulsen CA, Ware JL, Chen J, Vessella RL, Birnbaum RS: Proteolysis of insulin-like growth factor-binding protein-3 in the male reproductive tract. J Clin Endocrinol Metab 1996;81:618-624.
12. Cramer SD, Chen Z, Peehl DM: Prostate specific antigen cleaves parathyroid hormone-related protein in the PTH-like domain: Inactivation of PTHrP-stimulated cAMP accumulation in mouse osteoblasts. J Urol 1996;156:526-531.
13. Horoszewicz JS, Leong SS, Kawinski E, Karr JP, Rosenthal H, Chu TM, Mirand EA, Murphy GP: LNCaP model of human prostatic carcinoma. Cancer Res 1983;43:1809-1818.
14. Kaighn ME, Shankar Narayan K, Ohnuki Y, Lechner JF, Jones LW: Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest Urol 1979;17:16-23.
15. Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF: Isolation of a human prostate carcinoma cell line (DU 145). Int J Cancer 1978;21:274-281.
16. Corey E, Wegner SK, Stray JE, Corey MJ, Arfman EW, Lange PL, Vessella RL: Characterization of ten new monoclonal antibodies against prostate-specific antigen by affinity, specificity and function in sandwich assays. Int J Cancer 1997;71:1019-1028.
17. Wians FHJ: The 'correct' PSA concentration. Clin Chem 1997; 42:1882-1858.
18. Corey E, Wegner SK, Corey MJ, Vessella RL: Prostate-specific antigen: Characterization of epitopes by synthetic peptide mapping and inhibition studies. Clin Chem 1997;43:575-584.
19. Bostwick DG, Oesterling JE, MacLennam G, Pacelli A, Abrahamsson PA, Lilja H: PSA and alpha-1-antichymotrypsin expression in prostatic intraepithelial neoplasia and adenocarcinoma. J Urol [Suppl] 1996;155:1258.
20. Webber MM, Bello D, Quader S: Immortalized and tumorigenic adult human prostatic and epithelial cell lines: Characteristics and applications. Part 2. Tumorigenic cell lines. Prostate 1997; 30:58-64.
21. Garde SV, Sheth ARPAT, Pienta KJ: A comparative study on expression of prostatic inhibin peptide, prostate acid phosphatase and prostate specific antigen in androgen independent human and rat prostate carcinoma cell lines. Cancer Lett 1993;70: 159-166.
22. Cohen RJ, Haffejee Z, Steele GS, Nayler SJ: Advanced prostate cancer with normal serum prostate-specific antigen values. Arch Pathol Lab Med 1997;118:1123-1126.
23. Huber PR, Schmid HP, Mattarelli G, Strittmatter B, van Steenbrugge G-J, Maurer A: Serum-free prostate-specific antigen: Iso-enzymes in benign hyperplasia and cancer of the prostate. Prostate 1995;27:212-219.
24. Wu JT, Zhang P, Astill ME, Wilson MJ, Lyons BW, Wu LL, Stephenson R: PSA immunoreactivity detected in LNCaP cell media, breast tumor cytosol, and female serum. J Clin Lab Anal 1995;9:243-251.
25. Watt KW, Lee PJ, M'Timkulu T: Human prostate-specific antigen: structural and functional similarity with serine proteases. Proc Natl Acad Sci USA 1986;83:3166-3170.
26. Takayama T, Fujikawa K, Davie E: Characterization of the precursor of prostate-specific antigen: Activation by trypsin and by human glandular kallikrein. J Biol Chem 1997;272:21582-21588.
27. Grauer LS, Charlesworth MC, Saedi MS, Finlay JA, Liu R, Kuus-Reichel K, Young CY-F, Tindall DJ: Identification of human glandular kallikrein hK2 from LNCaP cells. J Androl 1996;17: 353-359.
28. Nguyen C, Dickason R, Song W, Young C, Tindall DJ, Slawin KM: RT-PCR for hK2 mRNA: A new assay for detecting circulating prostate cells. J Urol [Suppl] 1997;157:436.