Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency

Journal of Bacteriology

Volumn 180, Issue 2, 1998, Pages 377-387

  Zhang, Gang ^a,b   Deng, Enxin ^a   Baugh, Larry ^a   Kushner, Sidney R ^a  

^a University of Georgia ^*  (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; HELICASE;

ARTICLE; DNA DENATURATION; DNA REPAIR; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ISOLATION; NONHUMAN; PRIORITY JOURNAL;

EID: 0031936257     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.2.377-387.1998     Document Type: Article

References (53)

1. Abdel-Monem, M., M.-C. Chanal, and H. Hoffman-Berling. 1977. DNA unwinding enzyme II of Escherichia coli. 1. Purification and characterization of the ATPase activity. Eur. J. Biochem. 79:33-38.
2. Abdel-Monem, M., H. Durwald, and H. Hoffman-Berling. 1977. DNA unwinding enzyme II of Escherichia coli. 2. Characterization of the DNA unwinding activity. Eur. J. Biochem. 79:39-45.
3. Ali, J. A., and T. M. Lohman. 1997. Kinetic measurement of the step size of DNA unwinding by Escherichia coli UvrD helicase. Science 275:377-380.
4. Arai, N., and A. Kornberg. 1981. Rep protein as a helicase in an active, isolatable replication fork of duplex φX174 DNA. J. Biol. Chem. 256:5294-5298.
5. Arthur, H. M., and R. G. Lloyd. 1980. Hyper-recombination in uvrD mutants of Escherichia coli K-12. Mol. Gen. Genet. 180:185-191.
6. Bennett, W. F., N. F. Paoni, B. A. Keyt, D. Botstein, A. J. Jones, L. Presta, F. M. Wurm, and M. J. Zoller. 1991. High resolution analysis of functional determinants on human tissue-type plasminogen activator. J. Biol. Chem. 266:5191-5201.
7. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
8. Brosh, R. M., Jr., and S. W. Matson. 1995. Mutations in motif II of Escherichia coli DNA helicase II render the enzyme nonfunctional in both mismatch repair and excision repair with differential effects on the unwinding reaction. J. Bacteriol. 177:5612-5621.
9. Brosh, R. M., Jr., and S. W. Matson. 1996. A partially functional DNA helicase II mutant defective in forming stable binary complexes with ATP and DNA. J. Biol. Chem. 271:25360-25368.
10. Brosh, R. M., Jr., and S. W. Matson. 1997. A point mutation in Escherichia coli DNA helicase II renders the enzyme nonfunctional in two DNA repair pathways. J. Biol. Chem. 272:572-579.
11. Clark, A. J., and A. D. Margulies. 1965. Isolation and characterization of recombination-deficient mutants of Escherichia coli K12. Proc. Natl. Acad. Sci. USA 53:451-459.
12. George, J. W., R. M. Brosh, and S. W. Matson. 1994. A dominant negative allele of the Escherichia coli uvrD gene encoding DNA helicase II. A biochemical and genetic characterization. J. Mol. Biol. 235:424-435.
13. Gilchrist, C. A., and D. T. Denhardt. 1987. Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD. Nucleic Acids Res. 15:465-475.
14. Gorbalenya, A. E., and E. V. Koonin. 1990. Superfamily of UvrA-related NTP-binding proteins: implications for rational classification of repair/recombination systems. J. Mol. Biol. 213:583-591.
15. Gorbalenya, A. E., E. V. Koonin, A. P. Donchenko, and V. M. Blinov. 1988. A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination. FEBS Lett. 235:16-24.
16. Hodgman, T. C. 1988. A new superfamily of replicative proteins. Nature 333:22-23.
17. Kuhn, B., M. Abeld-Monem, H. Krell, and H. Hoffman-Berling. 1979. Evidence for two mechanisms for DNA unwinding catalyzed by DNA helicases. J. Biol. Chem. 254:11343-11350.
18. Kushner, S. R. 1974. In vivo studies of temperature-sensitive recB and recC mutants. J. Bacteriol. 120:1213-1218.
19. Kushner, S. R., and V. F. Maples. 1988. Purification and properties of the UvrD helicase, p. 509-527. In E. C. Friedberg and P. C. Hanawalt (ed.), DNA repair: a laboratory manual, vol. 3. Marcel Dekker, Inc., New York, N.Y.
20. Lohman, T. M., and K. P. Bjornson. 1996. Mechanisms of helicase-catalyzed DNA unwinding. Annu. Rev. Biochem. 65:169-214.
21. Luria, S. E., and M. Delbruck. 1943. Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491-511.
22. Maples, V. F., and S. R. Kushner. 1982. DNA repair in Escherichia coli: identification of the uvrD gene product. Proc. Natl. Acad. Sci. USA 79:5616-5620.
23. Matson, S. W. 1986. Escherichia coli DNA helicase II (uvrD gene product) translocates unidirectionally in a 3′ to 5′ direction. J. Biol. Chem. 262:10169-10175.
24. Matson, S. W., D. W. Bean, and J. W. George. 1994. DNA helicases: enzymes with essential roles in all aspects of DNA metabolism. Bioessays 16:13-22.
25. Matson, S. W., and K. A. Kaiser-Rogers. 1990. DNA helicases. Annu. Rev. Biochem. 59:289-329.
26. Matson, S. W., and C. C. Richardson. 1985. Nucleotide-dependent binding of the gene 4 protein of bacteriophage T7 to single-stranded DNA. J. Biol. Chem. 260:2281-2287.
27. Mendonca, V. M., K. Kaiser-Rogers, and S. W. Matson. 1993. Double heicase II (uvrD)-helicase IV (helD) deletion mutants are defective in the recombination pathways of Escherichia coli. J. Bacteriol. 175:4641-4651.
28. Miller, J. F. 1988. Bacterial electroporation. Bio-Rad Lab. Rep. 5.
29. Modrich, P. 1991. Mechanisms and biological effects of mismatch repair. Annu. Rev. Genet. 25:229-253.
30. Morel, P., J. A. Hejna, S. D. Ehrlich, and E. Cassuto. 1993. Antipairing and strand transferase activities of E. coli helicase II (UvrD). Nucleic Acids Res. 21:3205-3209.
31. Orren, D. K., C. P. Selby, J. E. Hearst, and A. Sancar. 1992. Postincision steps of nucleotide excision repair in Escherichia coli. Disassembly of the UvrBD-DNA complex by helicase II and DNA polymerase I. J. Biol. Chem. 267:780-788.
32. Peluso, R. W., and G. H. Rosenberg. 1987. Quantitative electrotransfer of proteins from sodium dodecyl sulfate-polyacrylamide gels onto positively charged nylon membranes. Anal. Biochem. 162:389-398.
33. Radman, M. 1988. Mismatch repair and genetic recombination, p. 169-192. In R. Kucherlapati and G. R. Smith (ed.), Genetic recombination. American Society for Microbiology, Washington, D.C.
34. Rayssingyier, C., D. S. Thaler, and M. Radman. 1989. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature 342:396-401.
35. Richet, E., and M. Kohiyama. 1976. Purification and characterization of a DNA-dependent ATPase from Escherichia coli. J. Biol. Chem. 251:808-812.
36. Richet, E., Y. Nishimura, Y. Hirota, and M. Kohiyama. 1983. Escherichia coli uvrD mutants with thermosensitive DNA-dependent adenosine triphosphatase I (helicase II). Mol. Gen. Genet. 192:378-385.
37. Kunyon, G. T., D. G. Bear, and T. M. Lohman. 1990. Escherichia coli helicase II (uvrD) protein initiates DNA unwinding at nicks and blunt ends. Proc. Natl. Acad. Sci. USA 87:6383-6387.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
39. Sancar, A. 1994. Mechanisms of DNA excision repair. Science 266:1954-1956.
40. Sibghat-Ullah, A., A. Sancar, and J. E. Hearst. 1990. The repair patch of E. coli (A)BC exinucleasc. Nucleic Acids Res. 18:5051-5053.
41. Siegel, E. C. 1981. Complementation studies with the repair-deficient uvrD3, uvrE156 and recL152 mutations in Escherichia coli. Mol. Gen. Genet. 184: 526-530.
42. Siegel, E. C. 1973. Ultraviolet-sensitive mutator strain of Escherichia coli K-12. J. Bacteriol. 113:145-160.
43. Siegel, E. C., and H. Race. 1981. Phenotypes of UV-sensitive uvrD3, recL152, and uvrD156 mutants of Escherichia coli. Mutat. Res. 83:49-59.
44. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 polymerase/ promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
45. Van Houten, B., H. Gamper, J. E. Hearst, and A. Sancar. 1988. Analysis of sequential steps of nucleotide excision repair in Escherichia coli using synthetic substrates containing single psoralen adducts. J. Biol. Chem. 263: 16553-16560.
46. Walker, J. M., M. Sarsate, M. J. Runswick, and N. J. Gay. 1982. Distantly related sequences in the A- and B-subunits of ATP synthase, myocin, kinases, and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1:945-951.
47. Wang, R.-F., and S. R. Kushner. 1991. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100:195-199.
48. Washburn, B. K., and S. R. Kushner. 1993. Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli. J. Bacteriol. 175:341-350.
49. Washburn, B. K., and S. R. Kushner. 1991. Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli. J. Bacteriol. 173:2569-2575.
50. Willetts, N. S., and A. J. Clark. 1969. Characteristics of some multiply recombination-deficient strains of Escherichia coli. J. Bacteriol. 100:231-239.
51. Zhang, G., L. R. Baugh, E. Deng, C. M. Hamilton, V. F. Maples, and S. R. Kushner. 1997. Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity. J. Bacteriol. 179:7544-7550.
52. Zieg, J., and S. R. Kushner. 1977. Analysis of genetic recombination between two partially deleted lactose operons of Escherichia coli K-12. J. Bacteriol. 131:123-132.
53. Zieg, J., V. F. Maples, and S. R. Kushner. 1978. Recombination levels of Escherichia coli K-12 mutants deficient in various replication, recombination, or repair genes. J. Bacteriol. 134:958-966.