Functional analysis of the ver gene using antisense transgenic wheat

Physiologia Plantarum

Volumn 102, Issue 1, 1998, Pages 87-92

  Chong, Kang ^a   Bao, Shi Lai ^b   Xu, Tao ^a   Tan, Ke Hui ^a   Liang, Tie Bing ^a   Zeng, Jun Zhi ^a   Huang, Hua Liang ^a   Xu, Ji ^a   Xu, Zhi Hong ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

Antisense RNA; Gene function; Pollen tube pathway; Triticum aestivum; Vernalization related cDNA; Winter wheat

Indexed keywords

ANTISENSE RNA; DNA AMPLIFICATION; FLOWERING; NORTHERN BLOTTING; PLASMID; POLYMERASE CHAIN REACTION; TRANSGENIC PLANT;

TRITICUM AESTIVUM;

EID: 0031917099     PISSN: 00319317     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3054.1998.1020112.x     Document Type: Article

References (22)

1. Brock, R. D. & Davidson, J. L. 1994. 5-Azacytidine and gamma rays partially substitute for cold treatment in vernalizing winter wheat. - Environ. Exp. Bot. 31: 195-199.
2. Burn, J. E., Babnall, D. J., Metzger, J. D., Dennis, E. S. & Peacock, W. J. 1993. DNA methylation, vernalization, and the initiation of flowering. - Proc. Natl. Acad. Sci. USA 90: 287-291.
3. Chong, K., Wang, L. P., Tan, K. H., Huang, H. L. & Liang, H. G. 1994. Molecular cloning and characterization of vernalization-related (ver) genes in winter wheat. - Physiol. Plant. 92: 511-515.
4. -, Tan, K. H., Huang, H. L. & Liang, H. G. 1995. Molecular cloning of a cDNA related to vernalization in winter wheat. - Sci. China Ser. B 38: 799-806.
5. -, Tan, K. H., Huang, H. L. & Liang, H. G. 1997. Sequence analysis of vernalization-related cDNA clone from winter wheat. - Acta Phytophysiol. Sin. 23: 99-102.
6. de la Pena, A., Lorz, H. & Schell, J. 1987. Transgenic rye plants obtained by injecting DNA into young floral tillers. - Nature 325: 274-276.
7. Edwards, K., Johnstone, C. & Thompson, C. 1991. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. - Nucleic Acids Res. 19: 1349.
8. Gong, Z. Z., Shen, W. F., Zhou, G. Y., Huang, J. Q. & Qian, S. Y. 1988. Introducing exogenous DNA into plants after pollination - DNA traverse pathway of pollen tube to reach embryonic sac. - Sci. China Ser. B 31: 1080-1084.
9. Gyllensten, U. B. & Erlich, H. A. 1988. Generation of single-stranded DNA by the polymerase chain reaction and its amplification to direct sequencing of HLA DQA locus. -Proc. Natl. Acad. Sci. USA 85: 7652-7656.
10. Hess, D. 1980. Investigations on intra-and interspecific transfer of anthocyanin genes using pollen as vector. - Z. Pflanzenphysiol. 98: 326-332.
11. -, Dressler, K. & Nimmrichter, R. 1990. Transformation experiments by pipetting Agrobacterium into the spikelets of wheat. - Plant Sci. 72: 233-244.
12. Jefferson, R. A. 1987. Assaying chimeric genes in plants: The GUS gene fusion system. - Plant Mol. Biol. Rep. 5: 387-405.
13. Luo, Z. H. & Wu, R. 1989. A simple method for transformation of rice via the pollen-tube pathway. - Plant Mol. Biol. Rep. 7: 69-77.
14. Putterill, J., Robson, F., Lee, K., Simon, R. & Coupland, G. 1995. The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. - Cell 80: 847-857.
15. Ronemus, M. J., Galbiati, M., Ticknor, C., Chen, J. & Dellaporta, S. L. 1996. Demethylation-induced developmental pleiotropy in Arabidopsis. - Science 273: 654-657.
16. Sambrook, J., Fritsch, E. F. & Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. - Cold Spring Harbor Laboratory Press, New York, NY. pp. 9.31-9.57. ISBN 0-87969-309-6.
17. Sommer, H., Beitran, J. P., Huijser, P., Pape, H., Lonnig, W. E., Saedler, H. & Schwarz-Sommer, Z. 1990. Deficiens, a homeotic gene is in the control of flower morphogenesis in Antirrhinum majus: The protein shows homology to transcription factors. - EMBO J. 9: 605-613.
18. Van der Krol, A. R., Lenting, P. E., Veenstra, J., van der Meer, I. M., Koes, R. E., Gerats, A. G. M., Mol, J. N. M. & Stuitje, A. R. 1988a. An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. -Nature 333: 866-869.
19. -, Mol, J. N. M. & Stuitje, A. R. 1988b. Antisense genes in plants: An overview. - Gene 72: 45-50.
20. Yanofsky, M. F., Ma, H., Bowman, J. L., Drews, G. N., Feldmann, K. A. & Meyerowitz, E. M. 1990. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. - Nature 346: 35-39.
21. Zeng, J. Z., Wang, D. J. & Wu, Y. Q. 1994. Transgenic wheat plants obtained with pollen-tube pathway method. - Sci. China Ser. B 37: 319-325.
22. Zhou, G. Y., Wang, J., Zeng, Y. S., Huang, J. G., Qian, S. Y. & Liu, G. L. 1983. Introduction of exogenous DNA into cotton embryos. - Methods Enzymol. 101: 433-481.