Screening for important base identities in the hairpin ribozyme by in vitro selection for cleavage

BioTechniques

Volumn 24, Issue 2, 1998, Pages 278-284

  Siwkowski, Andrew ^a   Humphrey, Margaret ^a   DeYoung, Mary Beth ^b   Hampel, Arnold ^a  

^a NORTHERN ILLINOIS UNIVERSITY   (United States)

^b GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOZYME;

ARTICLE; BASE PAIRING; GENE THERAPY; MUTAGENESIS; POINT MUTATION; RNA SEQUENCE; RNA STRUCTURE; STRUCTURE ACTIVITY RELATION;

EID: 0031915892     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98242st05     Document Type: Article

References (13)

1. Anderson, P., J. Monforte, R. Tritz, S. Nesbitt, J. Hearst and A. Hampel. 1994. Mutagenesis of the hairpin ribozyme. Nucleic Acids Res. 22:1096-1100.
2. Berzal-Herranz, A., S. Joseph and J.M. Burke. 1992. In vitro selection of active hairpin ribozymes by sequential RNA-catalyzed cleavage and ligation reactions. Genes Dev. 6:129-134.
3. Hampel, A. and R. Tritz. 1989. RNA catalytic properties of the minimum (-)sTRSV sequence. Biochemistry 23:4929-4933.
4. Lehman, N. and G.F. Joyce. 1993. Evolution in vitro of an RNA enzyme with altered metal dependence. Nature 361:182-185.
5. Long, D.M. and O.C. Uhlenbeck. 1994. Kinetic characterization of intramolecular and intermolecular hammerhead RNAs with stem II deletions. Proc. Natl. Acad. Sci. USA 91:6977-6981.
6. Milligan, J.F., D.R. Groebe, G.W. Witherell and O.C. Uhlenbeck. 1987. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15:8783-8798.
7. Nakamaye, K.L. and F. Eckstein. 1994. AUA-cleaving hammerhead ribozymes: attempted selection for improved cleavage. Biochemistry 33:1271-1277.
8. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
9. Schmidt, S., L. Beigelman, A. Karpeisky, N. Usman, U. Sorensen and M. Gait. 1996. Base and sugar requirements for RNA cleavage of essential nucleoside residues in internal loop B of the hairpin ribozyme: implications for secondary structure. Nucleic Acids Res. 24:573-581.
10. Siwkowski, A., R. Shippy and A. Hampel. 1997. Analysis of hairpin ribozyme base mutations in loops 2 and 4 and their effects on cis-cleavage in vitro. Biochemistry 36:3930-3940.
11. Vaish, N., P. Heaton and F. Eckstein. 1997. Isolation of hammerhead ribozymes with altered core sequences by in vitro selection. Biochemistry 36:6495-6501.
12. Williams, K. and D. Bartel. 1996. In vitro selection of catalytic RNA, p. 367-382. In F. Eckstein and D. Lilley (Eds.), Nucleic Acids and Molecular Biology, Vol 10:Catalytic RNA. Springer-Verlag, Berlin.
13. Yuan, Y. and S. Altman. 1994. Selection of guide sequences that direct efficient cleavage of mRNA by human ribonuclease P. Science 263:1269-1273.