Conditioned medium from cultured rat hepatocytes completely blocks induction of glutamine synthetase by dexamethasone in several rat liver epithelial cell lines

Cell Biology and Toxicology

Volumn 14, Issue 1, 1998, Pages 69-80

  Haupt, W ^a   Schrode, W ^a   Gebhardt, R ^a,b  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Dexamethasone; Epithelial cell lines; Glucocorticoids; Glutamine synthetase; Hepatocytes; Induction; Inhibition

Indexed keywords

DEXAMETHASONE; GLUTAMATE AMMONIA LIGASE;

ANIMAL CELL; ARTICLE; ASTROCYTE; CELL CULTURE; CELL LINE; COCULTURE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME INDUCTION; HEPATOMA CELL; IN VITRO STUDY; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; RAT; SWINE;

ANIMALS; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; DEXAMETHASONE; ENZYME INDUCTION; EPITHELIAL CELLS; FEMALE; GLUTAMATE-AMMONIA LIGASE; LIVER; MALE; RATS; RATS, SPRAGUE-DAWLEY; SWINE;

EID: 0031913628     PISSN: 07422091     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007420605997     Document Type: Article

References (44)

1. Beutler HO, Michal HG. In: Bergmeyer HU, ed. Methoden der enzymatischen Analyse. 3 Aufl. Bd. II. Weinheim: Verlag Chemie; 1974;1753-9.
2. Bhandari B, Wilson RH, Miller RE. Insulin and dexamethasone stimulate transcription of an amplified glutamine synthetase gene in Chinese hamster ovary cells. Mol Endocrinol. 1987;1:403-7.
3. Bigner SH, Friedman HS, Biegel JA et al. Specific chromosomal abnormalities characterise four established cell lines derived from malignant human gliomas. Acta Neuropathol. 1986;72:86-97.
4. Burger H-J, Gebhardt R, Mayer C, Mecke D. Hormonelle Induktion von Amino-säuretransportsystemen in kultivierten periportalen und perivenösen Rattenhepatozyten. Z Gastroenterol. 1987;25:66-7.
5. Burger H-J, Gebhardt R, Mayer C, Mecke D. Different capacities for amino acid transport in periportal and perivenous hepatocytes isolated by digitonin/collagenase perfusion. Hepatology. 1989;9:22-8.
6. Crook RB, Louie M, Deuel TF, Tomkins GM. Regulation of glutamine synthetase by dexamethasone in hepatoma tissue culture cells. J Biol Chem. 1978;253:6125-31.
7. Da Fonseca-Wollheim F. In: Bergmeyer HU, ed. Methoden der enzymatischen Analyse. 3 Aufl. Bd. II. Weinheim: Verlag Chemie; 1974;1850-3.
8. Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122:501-4.
9. Eagle H, Oyama VI, Levy M, Horton CL, Fleischmann R. The growth response of mammalian cells in tissue culture to L-glutamine and L-glutamic acid. J Biol Chem. 1956;218:607-16.
10. Feng B, Hilt DC, Max SR. Transcriptional regulation of glutamine synthetase gene expression by dexamethasone in L6 muscle cells. J Biol Chem. 1990;265:18702-6.
11. Gebhardt R, Jung W Biliary secretion of sodium fluorescein in primary cultures of adult rat hepatocytes and its stimulation by nicotinamide. J Cell Sci. 1982;56:233-44.
12. Gebhardt R, Mecke D. The role of growth hormone, dexamethasone and triiodothyronine in the regulation of glutamine synthetase in primary cultures of rat hepatocytes. Eur J Biochem. 1979;100:519-25.
13. Gebhardt R, Mecke D. Heterogeneous distribution of glutamine-synthetase among rat liver parenchymal cells in situ and in primary culture. EMBO J. 1983;2:567-70.
14. Gebhardt R, Mecke D. Cellular distribution and regulation of glutamine synthetase in liver. In: Hässinger D, Sies H, eds. Glutamine metabolism in mammalian tissues. Berlin: Springer-Verlag; 1984; 98-121.
15. Gebhardt R, Williams GM. Amino acid transport in established adult rat liver epithelial cell lines. Cell Biol Toxicol. 1986;2:9-20.
16. Gebhardt R, Gaunitz F, Mecke D. Heterogeneous (positional) expression of hepatic glutamine synthetase: features, regulation and implications for hepatocarcinogenesis. Adv Enzyme Regul. 1994;34:27-56.
17. Gebhardt R, Schrode W, Eisenmann-Tappe I. Cellular characteristics of epithelial cell lines from juvenile rat liver: selective induction of glutamine synthetase by dexamethasone. Cell Biol Toxicol. 1997. Cell Biol Toxicol. 1998;14:55-67.
18. Gebhardt R, Schrode W, Eisenmann-Tappe I. Cellular characteristics of epithelial cell lines from juvenile rat liver: selective induction of glutamine synthetase by dexamethasone. Cell Biol Toxicol. 1997. Cell Biol Toxicol. 1998;14:55-67.
19. Hallermayer K, Harmening C, Hamprecht B. Cellular localization and regulation of glutamine synthetase in primary cultures of brain cells from newborn mice. J Neurochem. 1981;37:43-52.
20. Kulka RG, Cohen H. Regulation of glutamine synthetase activity of hepatoma tissue culture cells by glutamine and dexamethasone. J Biol Chem. 1973;248:6738-43.
21. Levintow L. The glutamyltransferase activity of normal and neoplastic tissue. J Natl Cancer Inst. 1954;15:347-52.
22. Lindros KO, Penttilä KE. Digitonin-collagenase perfusion for efficient separation of periportal and perivenous hepatocytes. Biochem J. 1985;228:757-60.
23. Linser P, Moscona AA. Induction of glutamine synthetase in embryonic neural retina: localisation in müller fibres and dependence on cell interactions. Proc Natl Acad Sci USA. 1979;76:6476-80.
24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-75.
25. Lund P. In: Bergmeyer HU, ed. Methoden der enzymatischen Analyse. 3 Aufl. Bd. II. Weinheim: Verlag Chemie; 1974; 1767-9.
26. Max SR, Thomas JW, Banner C, Vitcovic L, Konagaya M, Konagaya J. Glucocorticoid-receptor mediated induction of glutamine synthetase in skeletal muscle cells in vitro. Endocrinology. 1987;120:1179-83.
27. McGinnis JF, de Vellis J. Glucocorticoid regulation in rat brain cell cultures. J Biol Chem. 1978;253:8483-92.
28. Miller RE, Hackenberger, Gershman H. Regulation of glutamine synthetase in cultured 3T3-L1 cells by insulin, hydrocortisone, and dibutyryl cyclic AMP. Proc Natl Acad Sci USA. 1978;75:1418-22.
29. Moscona M, Frenkel N, Moscona AA. Regulatory mechanisms in the induction of glutamine synthetase in the embryonic retina: immunochemical studies. Dev Biol. 1972;28:229-41.
30. Patel AJ, Hunt A, Tahourdin CSM. Regulation of in vivo glutamine synthetase activity by glucocorticoids in the developing rat brain. Dev Brain Res. 1983;10:83-91.
31. Pishak MR, Phillips AT. Glucocorticoid stimulation of glutamine synthetase production in cultured rat glioma cells. J Neurochem. 1980;34:866-72.
32. Quistorff B. Gluconeogenesis in periportal and perivenous hepatocytes of rat liver, isolated by a new high-yield digitonin-collagenase perfusion technique. Biochem J. 1985;229:221-6.
33. Rabinovitz M, Olson ME, Greenberg DM. Effect of glutamine analogues on amino acid incorporation into protein in some normal and neoplastic cells in vitro. Cancer Res. 1959; 19: 388-92.
34. Raina PN, Rosen F. Induction of glutamine synthetase by cortisol. Biochim Biophys Acta. 1968;165:470-5.
35. Saini K, Thomas P, Bhandari B. Hormonal regulation of stability of glutamine synthetase mRNA in cultured 3T3-L1 adipocytes. Biochem J. 1990;267:241-4.
36. Schrode W, Mecke D, Gebhardt R. Induction of glutamine synthetase in periportal hepatocytes by cocultivation with a liver epithelial cell line. Eur J Cell Biol. 1990;53:35-41.
37. Schuler M, Gebhardt R. Spontaneous induction of glutamine synthetase expression by pig hepatocytes cocultured with RL-ET-14 cells is completely inhibited by triiodothyronine. Eur J Cell Biol. 1994;63(supplement 40):66.
38. Tiemeier DC, Milman G. Regulation of glutamine synthetase in cultured Chinese hamster cells. J Biol Chem. 1972;247: 5722-7.
39. Vardimon L, Fox LL, Degenstein L, Moscona AA. Cell contacts are required for induction by Cortisol of glutamine synthetase gene transcription in the retina. Proc Natl Acad Sci USA. 1988;85:5981-5.
40. Wegner H, Scharek W, Bayer-Helms H, Gebhardt R. Different proliferative potential of rat and pig hepatocytes in pure primary culture and coculture. Eur J Cell Biol. 1992;58:411-7.
41. Wong BS, Dunn A. Possible growth hormone regulation of rat liver glutamine synthetase activity. Biochem Biophys Res Commun. 1977;79:876-84.
42. Wu C. Glutamine synthetase. III. Factors controlling its activity in the developing rat. Arch Biochem Biophys. 1964;106:394-401.
43. Wu C, Morris HP. Responsiveness of glutamine-metabolising enzymes in Morris hepatomas to metabolic modulations. Cancer Res. 1970;30(11):2675-84.
44. Zhang H, Young AP. Exogenous, but not endogenous, glucocorticoid receptor induces glutamine synthetase gene expression in early stage embryonic retina. J Biol Chem. 1993;268:2850-6.