Cellular characteristics of epithelial cell lines from juvenile rat liver: Selective induction of glutamine synthetase by dexamethasone

Cell Biology and Toxicology

Volumn 14, Issue 1, 1998, Pages 55-67

  Gebhardt, R ^a,b   Schrode, W ^a   Eisenmann Tappe, I ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Bile duct epithelial cells; Epithelial cell lines; Glucocorticoids; Glutamine synthetase; Hepatocytes; Induction

Indexed keywords

CYTOKERATIN 19; DEXAMETHASONE; GLUTAMATE AMMONIA LIGASE; VIMENTIN;

AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; BILE DUCT; CELL CLONE; CELL LINE; CELL TYPE; COCULTURE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; EPITHELIUM CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; LIVER CELL; MALE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAT;

ANIMALS; CELLS, CULTURED; CULTURE MEDIA; DEXAMETHASONE; ENZYME INDUCTION; EPITHELIAL CELLS; GLUTAMATE-AMMONIA LIGASE; IMMUNOHISTOCHEMISTRY; LIVER; MALE; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0031890550     PISSN: 07422091     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007468521927     Document Type: Article

References (64)

1. Arad G, Kulka RG. Effects of glutamine, methionine sulfone and dexamethasone on rates of synthesis of glutamine synthetase in cultured hepatoma cells. Biochim Biophys Acta. 1978;544:153-62.
2. Aterman K. The stem cells of the liver - a selective review. J Cancer Res Clin Oncol. 1992;118:87-115.
3. Barnes PR, Youngberg D, Kitos PA. Factors affecting the production of glutamine in cultured mouse cells. J Cell Physiol. 1971;77:135-44.
4. Bigner SH, Friedman HS, Biegel JA et al. Specific chromosomal abnormalities characterize four established cell lines derived from malignant human gliomas. Acta Neuropathol. 1986;72:86-97.
5. Borek C. Neoplastic transformation in vitro of a clone of adult liver epithelial cells into differentiated hepatoma-like cells under conditions of nutritional stress. Proc Natl Acad Sci USA. 1972;69:956-9.
6. Burger H-J, Gebhardt R, Mayer C, Mecke D. Different capacities for amino acid transport in periportal and perivenous hepatocytes isolated by digitonin/collagenase perfusion. Hepatology. 1989;9:22-8.
7. Chessebeuf M, Padieu P. Rat liver epithelial cell cultures in a serum-free medium: primary cultures and derived cell lines expressing differentiated functions. In Vitro. 1984;20:780-95.
8. Chessebeuf M, Exilie MF, Pirard G, Chatelain P, Padieu P. Effect of serum concentration on the chromosome number of a rat liver epithelial cell line. Cell Biol Int Rep. 1981;5: 399-408.
9. Chessebeuf M, Fischbach M, Padieu P. Time course study of L - tyrosine aminotransferase induction in serum-supplemented and serum-free rat liver cell lines. Cell Biol Toxicol. 1984;1:31-40.
10. Corlu A, Kneip B, Lhadi C et al. A plasma membrane protein is involved in cell contact-mediated regulation of tissue-specific genes in adult rat hepatocytes. J Cell Biol. 1991;115:505-15.
11. Cotariu D, Barr-Nea L, Papo N, Zaidman JI. Induction of γ-glutamyl transferase by dexamethasone in cultured fetal rat hepatocytes. Enzyme. 1988;40:212-6.
12. Crook RB, Louis M, Deuel TF, Tomkins GM. Regulation of glutamine synthetase by dexamethasone in hepatoma tissue culture cells. J Biol Chem. 1978;253:6125-31.
13. Eisenmann-Tappe I, Wizigmann S, Gebhardt R. Glutamate uptake in primary cultures of biliary epithelial cells from normal rat liver. Cell Biol Toxicol. 1991;4:315-25.
14. Furukawa K, Shimada T, England P, Mochizuki Y, Williams GM. Enrichment and characterization of clonogenic epithelial cells from adult rat liver and initiation of epithelial cell strains. In Vitro Cell Dev Biol. 1987;23:339-48.
15. Gall JAM, Bhathal PS. The isolation of intrahepatic biliary epithelial cells from normal rat livers. Cell Biol Int Rep. 1985;9:315-22.
16. Gebhardt R. Disappearance of visible bile canaliculi caused by vinblastine in primary cultures of rat hepatocytes. Exp Cell Res. 1983;144:218-23.
17. Gebhardt R. Improved drug metabolizing capacity of hepatocytes co-cultured with epithelial cells and maintained in a Perifusion system. In: Reinhardt Ch, ed. Alternatives to animal testing. Weinheim: Verlag Chemie; 1994:141-6.
18. 4. J Hepatol. 1987;4:381-9.
19. Gebhardt R, Jung W. Biliary secretion of sodium fluorescein in primary cultures of adult rat hepatocytes and its stimulation by nicotinamide. J Cell Sci. 1982;56:233-44.
20. Gebhardt R, Mecke D. Perifused monolayer cultures of rat hepatocytes as an improved in vitro system for studies on ureogenesis. Exp Cell Res. 1979a;124:349-59.
21. Gebhardt R, Mecke D. The role of growth hormone, dexamethasone and triiodothyronine in the regulation of glutamine synthetase in primary cultures of rat hepatocytes. Eur J Biochem. 1979b;100:519-25.
22. Gebhardt R, Mecke D. Heterogeneous distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture. EMBO J. 1983;2:567-70.
23. Gebhardt R, Mecke D. Cellular distribution and regulation of glutamine synthetase in liver. In: Häussinger D, Sies H, eds. Glutamine metabolism in mammalian tissues. Berlin: Springer-Verlag; 1984:98-121.
24. Gebhardt R, Schäfer-Degenhart I. Monoclonal antibodies directed against rat liver epithelial cell lines selectively recognize bile duct epithelium in livers of adult rats. Cell Biol Toxicol. 1988;4:379-92.
25. Gebhardt R, Williams GM. Amino acid transport in established adult rat liver epithelial cell lines. Cell Biol Toxicol. 1986;2:9-20.
26. Gebhardt R, Burger H-J, Heini H, Schreiber K-L, Mecke D. Alterations of hepatic enzyme levels and of the acinar distribution of glutamine synthetase in response to experimental liver injury in the rat. Hepatology. 1988;8:822-30.
27. Gebhardt R, Fitzke H, Fausel M, Eisenmann-Tappe I, Mecke D. Influence of hormones and drugs on glutathione-S-transferase levels in primary culture of adult rat hepatocytes. Cell Biol Toxicol. 1990;6:369-72.
28. Gebhardt R, Gaunitz F, Mecke D. Heterogeneous (positional) expression of glutamine synthetase: features, regulation and implications for hepatocarcinogenesis. Adv Enzyme Regul. 1994;34:27-56.
29. Gebhardt R, Williams GM. Glutamine synthetase and hepatocarcinogenesis. Carcinogenesis. 1995;16:1673-81.
30. Gebhardt R, Wegner H, Alber J. Perifusion of co-cultured hepatocytes: optimization of studies on drug metabolism and cytotoxicity in vitro. Cell Biol Toxicol. 1996;12:57-68.
31. Gerschenson LE, Davidson MB, Andersson M. Hormonal regulation of rat liver cells cultured in chemically defined medium. Dexamethasone and insulin effects on different forms of tyrosine 2-oxoglutarate transaminase. Eur J Biochem. 1974;41:139-48.
32. Grisham JW. Cell types in long-term propagable cultures in rat liver. Ann NY Acad Sci USA. 1980;349:128-37.
33. Guguen-Guillouzo C, Clement B, Baffet G et al. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes cocultured with another liver epithelial cell type. Exp Cell Res. 1983;143:47-54.
34. Guguen-Guillouzo C. Role of homotypic and heterotypic cell interactions in expression of specific functions by cultured hepatocytes. In: Guillouzo A, Guguen-Guillouzo C, eds. Research in isolated and cultured hepatocytes. Paris: John Libbey Eurotext/INSERM; 1986:159-284.
35. Hallermayer K, Harmening C, Hamprecht B. Cellular localization and regulation of glutamine synthetase in primary cultures of brain cells from newborn mice. J Neurochem. 1981;37:43-52.
36. Haupt W, Schrode W, Gebhardt R. Conditioned medium from cultured rat hepatocytes completely blocks induction of glutamine synthetase by dexamethasone in several epithelial cell lines. Cell Biol Toxicol. 1998;14:69-80.
37. Iype PT. Cultures from adult rat liver cells. I. Establishment of monolayer cell-cultures from normal liver. J Cell Physiol. 1971;78:281-8.
38. Karsten U, Neupert G, Thust R. Characterization of an established epithelial cell line (RL-19) from rat liver. Exp Pathol. 1976;12:88-99.
39. Keppler D, Decker K. Glykogen. Bestimmung mit Amyloglucosidase. In: Bergmeyer HU, ed. Methoden der Enzymatischen Analyse. 3d edn. vol 2. Weinheim: Verlag Chemie; 1974:1171-6.
40. Kulka RG, Cohen H. Regulation of glutamine synthetase activity of hepatoma tissue culture cells by glutamine and dexamethasone. J Biol Chem. 1973;248:6738-43.
41. Levintow L. The glutamyltransferase activity of normal and neoplastic tissue. J Natl Cancer Inst. 1954;15:347-52.
42. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol regeant. J Biol Chem. 1951;193:265-75.
43. Malan-Shibley L, Iype PT. The influence of culture conditions on cell morphology and tyrosine aminotransferase levels in rat liver epithelial cell lines. Exp Cell Res. 1981;131:363-71.
44. Mayer D, Schäfer B. Biochemical and morphological characterization of glycogen-storing epithelial liver cell lines. Exp Cell Res. 1982;138:1-14.
45. Marceau N, Germain L, Goyette R, Noel M, Gourdeau H. Cell of origin of distinct cultured rat liver epithelial cells, as typed by epithelial keratin and surface component selective expression. Biochem Cell Biol. 1986;64:788-802.
46. McGinnis JF, De Vellis J. Glucocorticoid regulation in rat brain cell cultures. J Biol Chem. 1978;253:8483-92.
47. Mesnil M, Fraslin J-M, Piccoli C, Yamasaki H, Guguen-Guillouzo C. Cell contact but not junctional communication (dye coupling) with biliary epithelial cells is required for hepatocytes to maintain differentiated functions. Exp Cell Res. 1987;173:524-33.
48. Nakamura T, Yoshimoto K, Nakayama Y, Tomita Y, Ichihara A. Reciprocal modulation of growth and differentiated functions of mature rat hepatocytes in primary culture by cell-cell contact and cell membranes. Proc Natl Acad Sci USA. 1983;80:7229-33.
49. Neupert G, Langbein L, Karsten U Characterization of established epitheloid cell lines derived from rat liver: expression of cytokeratin filaments. Exp Pathol. 1987;31:161-7.
50. Nolan EM, Masters JN, Dunn A. Growth hormone regulation of hepatic glutamine synthetase mRNA levels in rats. Mol Cell Endocrinol. 1990;69:101-10.
51. Rogiers V, Vercruysse A. Rat hepatocyte cultures and co-cultures in biotransformation studies of xenobiotics. Toxicology. 1993;82:193-208.
52. San RHC, Aspia MF, Soiefer AI, Maslansky CJ, Rice JM, Williams GM. A survey of growth in soft agar and cell surface properties as markers for transformation in adult rat liver epithelial-like cell cultures. Cancer Res. 1979;39: 1026-34.
53. Schrenk D, Eisenmann-Tappe I, Gebhardt R et al. Drug metabolizing enzyme activities in rat liver epithelial cell lines, hepatocytes and bile duct cells. Biochem Pharmacol. 1991;41:1751-7.
54. Schrode W, Mecke D, Gebhardt R. Induction of glutamine synthetase in periportal hepatocytes by cocultivation with a liver epithelial cell line. Eur J Cell Biol. 1990;53:35-41.
55. Thompson EB, Tomkins GM, Curran JF. Induction of tyrosine ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line. Proc Natl Acad Sci USA. 1966;56:296-303.
56. Tsao MS, Liu C. Inhibition of growth of early passage normal rat liver epithelial cell lines by epidermal growth factor. Lab Invest. 1986;58:636-42.
57. Tsao MS, Smith JD, Nelson KG, Grisham JW. A diploid epithelial cell line from normal adult rat liver with phenotypic properties of "oval" cells. Exp Cell Res. 1984;154:38-52.
58. Volk A, Michalopoulos G, Weidner M, Gebhardt R. Different proliferative response of periportal and pericentral rat hepatocytes to hepatocyte growth factor. Biochem Biophys Res Commun. 1995;207:578-84.
59. Weinstein IB, Orenstein JM, Gebert R. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 1975;35:253-63.
60. Williams GM. Primary and long-term culture of adult rat liver epithelial cells. Methods Cell Biol. 1976;14:357-64.
61. Williams GM, Gunn JM. Long-term cell culture of adult rat liver epithelial cells. Exp Cell Res. 1974;89:139-42.
62. Williams GM, Weisburger EK, Weisburger JH. Isolation and long-term cell culture of epithelial-like cells from rat liver. Exp Cell Res. 1971;69:106-12.
63. Williams GM, Stromberg K, Kroes R. Cytochemical and ultrastructural alterations associated with confluent growth in cell cultures of epithelial-like cells from rat liver. Lab Invest. 1973;29:293-303.
64. Wong BS, Chenoweth ME, Dunn A. Possible growth hormone control of liver glutamine synthetase activity in rats. Endocrinology. 1980;106:268-74.