Non-uniform cellular packing of the stratum corneum and permeability barrier function of intact skin: A high-resolution confocal laser scanning microscopy study using highly deformable vesicles (Transfersomes)

British Journal of Dermatology

Volumn 138, Issue 4, 1998, Pages 583-592

  Schatzlein A ^a,b   Cevc, G ^a,c  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b IDEA   (Germany)

^c CRC Department of Medical Oncology   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

LIPID; TRANSFERSOME; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CELL ULTRASTRUCTURE; CONFOCAL LASER MICROSCOPY; HYDROPHILICITY; KERATINOCYTE; LIPID VESICLE; MEMBRANE PERMEABILITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; SKIN PERMEABILITY; STRATUM CORNEUM; TRANSDERMAL DRUG ADMINISTRATION;

ANIMALS; DRUG CARRIERS; EPIDERMIS; FLUORESCENT DYES; LIPIDS; MICE; MICE, INBRED STRAINS; MICE, NUDE; MICROSCOPY, CONFOCAL; PERMEABILITY; RHODAMINES; SKIN ABSORPTION;

EID: 0031898536     PISSN: 00070963     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2133.1998.02166.x     Document Type: Article

References (31)

1. Christophers E, Wolff HH, Laurence EB. The formation of epidermal cell columns. J Invest Dermatol 1974; 62: 555-9.
2. Swartzendruber DC, Wertz PD, Kitko DJ et al. Molecular models of intercellular lipid lamellae in mammalian stratum corneum. J Invest Dermatol 1989; 92: 251-7.
3. Wertz PW. Skin lipids in health and disease. In: Phospholipids: Characterization, Metabolism and Novel Biological Applications (Cevc G, Paltauf F. eds). Champaign: AOCS Press 1995: 139-58.
4. Swartzendruber DC, Wertz PW, Madison KC, Downing DT. Evidence that the corneocyte has a chemically bound lipid envelope. J Invest Dermatol 1987; 88: 709-13.
5. Wertz PW, Swartzendruber DC, Kitko DJ et al. The role of the corneocyte lipid envelopes in cohesion of the stratum corneum. J Invest Dermatol 1989; 93: 169-72.
6. Hadgraft J, Guy RH (eds) Transdermal Drug Delivery. Developmental Issues and Research Initiatives. New York: Marcel Dekker 1989.
7. Potts RO, Guy RH. Prediciting skin permeability. Pharm Res 1992; 9: 663-9.
8. Hofland HEJ, Bouwstra JA, Spies F et al. Interactions between non-ionic surfactant vesicles and human stratum corneum in vitro. J Liposome Res 1995; 5: 241-64.
9. Simonetti O, Hoogstraate AJ, Bialik W et al. Visualization of diffusion pathways across the stratum corneum of native and in-vitro reconstructed epidermis by confocal laser scanning microscopy. Arch Dermatol Res 1995; 287: 465-75.
10. Zellmer S, Pfeil W, Lasch J. Interaction of phosphatidylcholine liposomes with the human stratum corneum. Biochim Biophys Acta 1995; 1237: 176-82.
11. Veiro JA, Cummins PG. Imaging of skin epidermis from various origins using confocal laser scanning microscopy. Dermatology 1994; 189: 16-22.
12. Rajadhyaksha M, Grossman M, Esterowitz D et al. In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast. J Invest Dermatol 1995; 104: 946-52.
13. Cevc G, Blume G, Schätzlein A, Gebauer D. The skin: a pathway for systemic treatment with patches and lipid-based agent carriers. Adv Drug Delivery Rev 1996; 18: 349-78.
14. Cevc G, Schätzlein A, Blume G. Transdermal drug carriers: basic properties, optimization and transfer-efficiency in the case of epicutaneously applied peptides. J Contr Rel 1995; 36: 3-16.
15. Cevc G. Drug delivery across the skin. Exp Opinion Invest Drugs 1997; 6: 1887-1937.
16. Cevc G. Transfersomes, liposomes and other lipid suspensions on the skin: permeation enhancement, vesicle penetration and transdermal drug delivery. Crit Rev Ther Drug Carrier Systems 1996; 13: 257-388.
17. Cevc G. European Patent Number, 91 114 163 8-2114.
18. Cevc G, Blume G. Lipid vesicles penetrate into intact skin owing to transdermal osmotic gradients and hydration force. Biochim Biophys Acta 1992; 1104: 226-32.
19. Cevc G. Material transport across permeability barriers by means of lipid vesicles. In: Handbook of Biological Physics. Structure and Dynamics of Membranes (Lipowsky R, Sackmann E, eds), Vol. 1A. Amsterdam: Elsevier, 1995; 441-56.
20. Pawley JB. (ed.) Handbook of Biological Confocal Microscopy. New York: Plenum 1990.
21. Majlof L, Forsgren PO. Confocal microscopy, important considerations for accurate imaging. In: Cell Biological Applications of Confocal Microscopy (Matsumoto B, ed.). San Diego: Academic Press 1993; 79-95.
22. Warner RR, Myers MC, Taylor DT. Electron probe analysis of human skin: determination of the water concentration profile. J Invest Dermatol 1988; 90: 218-24.
23. Fartasch M, Bassukas ID, Diepgen TL. Structural relationship between epidermal lipid lamellae, lamellar bodies and desmosomes in human epidermis: an ultrastructural study. Br J Dermatol 1993: 128: 1-9.
24. Lasch J, Laub R, Wohlrab W. How deep do intact liposomes penetrate into human skin? J Contr Rel 1992: 18: 55-8.
25. Schätzlein A, Cevc G. Skin penetration by phospholipid vesicles, Transfersomes. as visualized by means of the confocal laser scanning microscopy. In: Phospholipids: Characterization. Metabolism and Novel Biological Applications (Cevc G, Paltauf F, eds). Champaign: AOCS Press 1995; 191-209.
26. Schreier H, Bouwstra J. Liposomes and niosomes as topical drug carriers: dermal and transdermal drug delivery. J Contr Rel 1994; 30: 1-15.
27. White SH, Mirejovsky D, King GI. Structure of lamellar lipid domains and corneocyte envelopes of murine stratum corneum. An X-ray diffraction study. Biochemistry 1988; 27: 3725-32.
28. Bouwstra JA, Goris GS, van der Spek JA, Bras W. Structural investigations of human stratum corneum by small angle X-ray scattering. J Invest Dermatol 1991; 97: 1005-12.
29. Gompper G, Kroll DM. Driven transport of fluid vesicles through narrow pores. Phys Rev 1995; E52: 4198-208.
30. Warner RR, Lilly NA. Correlation of water content with ultrastructure in the stratum corneum. In: Bioengeering of the Skin (Elsner P, Berardesca E, Maibach HI. eds). Boca Raton: CRC Press, 1994; 3-12.
31. Christophers E, Sterry W, Schubert C, Bräuer H (eds) Elementa Dermatologica. Frankfurt: Cassella-Riedel Pharma. 1987.